公开(公告)号：EP1715033A1

公开(公告)日：2006-10-25

申请号：EP05710079.4

申请日：2005-02-10

申请人： ReproCELL Inc. ,  ASAHI TECHNO GLASS COSPORATION ,  TANABE SEIYAKU CO., LTD.

发明人： NAKATSUJI, Norio ,  SUEMORI, Hirofumi ,  ASAKA, Isao, Asahi Techno Glass Corporation ,  SUGAI, Harumi, Asahi Techno Glass Corporation ,  OKAMOTO, Reiko, Asahi Techno Glass Corporation

IPC分类号： C12N5/06 ,  A61K35/36 ,  A61K48/00 ,  A61L27/00

CPC分类号： C12N5/0652 ,  C12N5/0606 ,  C12N2500/44 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2502/13 ,  C12N2502/1323 ,  C12N2533/54

摘要： A culture medium for preparation of feeder cells for embryonic stem cells, which can efficiently establish feeder cells for use in culture of embryonic stem cells including human's from limited donor-derived materials and culture them in a condition of a reduced risk of infection, is provided. Further, a preparation method of feeder cells, which is relatively safe even when subjected to coculture with embryonic stem cells including human's, and the resulting feeder cells therefrom are provided. With the culture medium for preparation of feeder cells for embryonic stem cells comprising at least a serum albumin and insulin in a basal medium, a cell population comprising at least one kind of cells selected from fetal skin fibroblasts, fetal myofibroblasts, fetal lung fibroblasts, fetal epithelial cells, fetal endothelial cells, adult skin fibroblasts, adult lung fibroblasts, adult epithelial cells and endothelial cells which can become feeder cells for embryonic stem cells can be stably proliferated.

摘要翻译： 一种用于制备用于胚胎干细胞的饲养细胞的培养基，其可以有效地建立用于胚胎干细胞培养的饲养细胞，所述胚胎干细胞包括来自有限供体来源的材料的人类，并且在感染风险降低的条件下培养它们 。 此外，提供了饲养细胞的制备方法，即使在与包括人的胚胎干细胞进行共培养的情况下也是相对安全的，以及由此产生的饲养细胞。 使用包含至少一种选自胎儿皮肤成纤维细胞，胎儿肌成纤维细胞，胎儿肺成纤维细胞，胎儿成纤维细胞，胎儿成纤维细胞，胎儿成纤维细胞，胎儿成纤维细胞 可以稳定地增殖上皮细胞，胎儿内皮细胞，成年皮肤成纤维细胞，成年肺成纤维细胞，成年上皮细胞和成为胚胎干细胞的饲养细胞的内皮细胞。