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    PROTEIN SUSTAINING UNDIFFERENTIATED STEM CELLS AS SUCH
    2.
    发明公开
    PROTEIN SUSTAINING UNDIFFERENTIATED STEM CELLS AS SUCH 审中-公开
    不孕症STAMMZELLEN ALS SOLCHE AUFRECHTERHALTENDES蛋白

    公开(公告)号：EP1489168A1

    公开(公告)日：2004-12-22

    申请号：EP02703964.3

    申请日：2002-03-11

    申请人： ReproCELL Inc. ,  Ema, Hideo

    发明人： EMA, Hideo ,  NAKAUCHI, Hiromitsu ,  OSAWA, Mitsujiro

    IPC分类号： C12N15/09 ,  C07K14/475 ,  C12N5/10 ,  A61K38/17 ,  A61P43/00 ,  A61K35/14 ,  A61K35/12

    CPC分类号： C12N5/0647 ,  C07K14/47 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2501/415

    摘要： The sustaining stem cells for example, hematopoietic stem cells while holding in the undifferentiated state and maintaining pluripotency by providing a polypeptide having a WIF domain for example, WIF-1 to the stem cells has been successfully achieved. This polypeptide preferably also contains an EGF-like repeat. This result has been achieved by providing a polypeptide preferably having the WIF domain together with a stem cell survival factor such as SCF. Thus, it becomes possible to easily provide a large amount of stem cells for example, hematopoietic stem cells containing functional precursor cells. It has also become possible to more efficiently transfer a gene into the stem cells. Because it is homogeneous and almost free from impurities, the stem cell composition can remarkably relieve side effects for example, infection, which occur in conventional therapies using hematopoietic stem cells.

    摘要翻译： 维持性干细胞例如通过向干细胞提供具有WIF结构域（例如WIF-1）的多肽而保持未分化状态并保持多能性的造血干细胞已被成功地实现。 该多肽优选还含有EGF样重复。 通过提供优选将WIF结构域与干细胞存活因子如SCF一起提供的多肽来实现该结果。 因此，可以容易地提供大量的干细胞，例如含有功能性前体细胞的造血干细胞。 也可以更有效地将基因转移到干细胞中。 因为它是均质的并且几乎没有杂质，所以干细胞组合物可以显着地减轻使用造血干细胞的常规疗法中发生的副作用，例如感染。

    CELL MEASURING VESSEL, EXTRACELLULAR POTENTIAL MEASURING METHOD, AND CHEMICAL TESTING METHOD
    4.
    发明公开
    CELL MEASURING VESSEL, EXTRACELLULAR POTENTIAL MEASURING METHOD, AND CHEMICAL TESTING METHOD 有权
    ZELLMESSGEFÄSS，EXTRAZELLULÄRES潜在客户化解CHEMISCHES TESTVERFAHREN

    公开(公告)号：EP2213721A1

    公开(公告)日：2010-08-04

    申请号：EP08832478.5

    申请日：2008-09-17

    申请人： Biotec Co., Ltd. ,  Reprocell, Inc.

    发明人： ASAI, Yasuyuki ,  NAGAKURA, Makoto ,  FUKAMI, Takeaki ,  OOSUMI, Keisuke

    IPC分类号： C12M1/34 ,  C12Q1/02 ,  G01N27/28 ,  G01N27/416

    CPC分类号： B01L3/5085 ,  B01L2300/0645 ,  B01L2300/0829 ,  C12M41/36 ,  G01N33/4836 ,  G01N33/48728

    摘要： The cell observation using a conventional well plate takes much costs. Each well 3 being opened at a top plate 2a of the well plate 1 has a diameter reduced portion 32 which inner circumferential surface 32a is conically hollowed. At a lower portion of the diameter reduced portion 32 is formed an inserting hole 33. In a detecting portion 4, an outer circumferential surface of a round-bar reference electrode 41 is covered by an insulating portion 42. The detecting portion 4 has an upper end portion 4a and a lower end portion 4b which outer circumferential surface is exposed to an outside without being covered by an insulating portion 42. On an upper side above the lower end portion 4b is formed a diameter expanded portion 4c in which an outer circumferential surface of the insulating portion 42 is covered with a measuring electrode 43. The detecting portion 4 is fixed to a well 3 whose diameter expanded portion 4c is inserted into an inserting hole 33 and its upper end portion 4a is contained in the well 3. In the detecting portion 4, the upper end surface 43a of the measuring electrode 43 is communicated with an inner circumferential surface 32a of the diameter reduced portion 32 positioned in an edge surrounding portion of the inserting hole 33.

    摘要翻译： 使用常规孔板的细胞观察需要很多成本。 在井板1的顶板2a上打开的各孔3具有直径减小部32，该内周面32a是锥形中空的。 在直径减小部32的下部形成有插入孔33.在检测部4中，圆棒状基准电极41的外周面被绝缘部42覆盖。检测部4具有上部 端部4a和下端部4b，该外周面暴露于外部而不被绝缘部42覆盖。在下端部4b上方的上侧形成有直径扩大部4c，其中外周面 绝缘部分42被测量电极43覆盖。检测部分4固定到其直径扩大部分4c插入插入孔33并且其上端部分4a容纳在井3中的井3中。在 检测部分4，测量电极43的上端表面43a与位于边缘周边的直径减小部分32的内圆周表面32a相连通 g部分插入孔33。

    EXPANSION AGENTS FOR STEM CELLS
    5.
    发明公开
    EXPANSION AGENTS FOR STEM CELLS 审中-公开
    手段扩展干细胞

    公开(公告)号：EP1560916A1

    公开(公告)日：2005-08-10

    申请号：EP03758840.7

    申请日：2003-10-23

    申请人： ReproCELL Inc.

    发明人： NAKAUCHI, Hiromitsu ,  IWAMA, Atsushi

    IPC分类号： C12N5/06 ,  C07K14/47

    CPC分类号： C12N5/0647 ,  C07K14/4705 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/26

    摘要： The present invention relates to a method and substance for maintaining the pluripotency and self-replication ability of a stem cell, such as a hematopoietic stem cell, while keeping it undifferentiated. Specifically, the present invention provides a composition for maintaining the expansion or pluripotency of a stem cell, comprising active STAT5, and a method using the same. STAT5 may be in the form of a protein or a nucleic acid. The composition may contain a cellular physiologically active substance (e.g., SCF, TPO, Flt-3L, etc.). The present invention also relates to a cell, a tissue and an organ prepared from the stem cell.

    METHOD FOR REMOVING DESIRED CHROMOSOME AND TAILOR-MADE MEDICAL TREATMENT UTILIZING THE SAME
    7.
    发明公开
    METHOD FOR REMOVING DESIRED CHROMOSOME AND TAILOR-MADE MEDICAL TREATMENT UTILIZING THE SAME 审中-公开
    方法除去其IN USE所需的染色体和量身定制的治疗医疗

    公开(公告)号：EP1842909A1

    公开(公告)日：2007-10-10

    申请号：EP06702679.9

    申请日：2006-01-12

    申请人： President, Kyoto University ,  ReproCELL Inc.

    发明人： TADA, Takashi c/o Institute for Frontier Medical ,  NAKATSUJI, Norio c/o Institute for Frontier Medica ,  MATSUMURA, Hiroyuki c/o Institute for Frontier Med ,  TADA, Masako

    IPC分类号： C12N15/00 ,  A61K35/48 ,  A61L27/00 ,  C12N5/10 ,  C12N15/09

    CPC分类号： A61K35/48 ,  C12N5/0081 ,  C12N5/12 ,  C12N15/907 ,  C12N2510/00 ,  C12N2800/30 ,  C12N2840/203

    摘要： It is intended to provide a regenerable cell in which a desired chromosome has been deleted, a method for producing the cell, and a gene cassette and a kit to be used for the method. More particularly, it is intended to obtain an individual corresponding pluripotent stem cell easily and simply. More specifically, it is intended to efficiently establish a cell, tissue or organ that can be a donor for treating a disease without causing immune rejection response, without newly obtaining and establishing a stem cell such as ES cell from the individual, and without using an ovum as a material. It was achieved by a gene cassette in which not two recombinase recognition sites in a cis orientation are inserted, but one recombinase recognition site or two recombinase recognition sites in an opposite orientation is/are inserted and a marker gene is connected, and by application of the gene cassette to a cell fusion technique.

    摘要翻译： 它的目的是提供可再生的细胞，其中，期望的染色体已被删除，用于生产细胞的方法，以及基因盒和试剂盒可以用于该方法。 更具体地，它的目的在单独的相应的多能干细胞容易且简单地得到。 更具体地，其目的是为了有效地建立细胞，组织或器官确实可以用于治疗的疾病，而不会引起免疫排斥反应，而不用新获取并建立的干细胞的供体：如从各个ES细胞，并且不使用在 卵子作为材料。 它是由其中在顺方向不是两个重组酶识别位点插入的基因盒实现的，但是一个重组酶识别位点或在相反的方向两个重组酶识别位点被插入/和标记基因连接，并通过应用 所述基因盒的细胞融合技术。

    MEDIUM FOR PREPARING FEEDER CELLS FOR EMBRYONIC STEM CELLS AND FEEDER CELLS
    9.
    发明公开
    MEDIUM FOR PREPARING FEEDER CELLS FOR EMBRYONIC STEM CELLS AND FEEDER CELLS 审中-公开
    中号ZURPRÄPARATIONVON FEEDER-ZELLENFÜREMBRYONALE STAMMZELLEN SOWIE FEEDER-ZELLEN

    公开(公告)号：EP1715033A1

    公开(公告)日：2006-10-25

    申请号：EP05710079.4

    申请日：2005-02-10

    申请人： ReproCELL Inc. ,  ASAHI TECHNO GLASS COSPORATION ,  TANABE SEIYAKU CO., LTD.

    发明人： NAKATSUJI, Norio ,  SUEMORI, Hirofumi ,  ASAKA, Isao, Asahi Techno Glass Corporation ,  SUGAI, Harumi, Asahi Techno Glass Corporation ,  OKAMOTO, Reiko, Asahi Techno Glass Corporation

    IPC分类号： C12N5/06 ,  A61K35/36 ,  A61K48/00 ,  A61L27/00

    CPC分类号： C12N5/0652 ,  C12N5/0606 ,  C12N2500/44 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2502/13 ,  C12N2502/1323 ,  C12N2533/54

    摘要： A culture medium for preparation of feeder cells for embryonic stem cells, which can efficiently establish feeder cells for use in culture of embryonic stem cells including human's from limited donor-derived materials and culture them in a condition of a reduced risk of infection, is provided. Further, a preparation method of feeder cells, which is relatively safe even when subjected to coculture with embryonic stem cells including human's, and the resulting feeder cells therefrom are provided. With the culture medium for preparation of feeder cells for embryonic stem cells comprising at least a serum albumin and insulin in a basal medium, a cell population comprising at least one kind of cells selected from fetal skin fibroblasts, fetal myofibroblasts, fetal lung fibroblasts, fetal epithelial cells, fetal endothelial cells, adult skin fibroblasts, adult lung fibroblasts, adult epithelial cells and endothelial cells which can become feeder cells for embryonic stem cells can be stably proliferated.

    摘要翻译： 一种用于制备用于胚胎干细胞的饲养细胞的培养基，其可以有效地建立用于胚胎干细胞培养的饲养细胞，所述胚胎干细胞包括来自有限供体来源的材料的人类，并且在感染风险降低的条件下培养它们 。 此外，提供了饲养细胞的制备方法，即使在与包括人的胚胎干细胞进行共培养的情况下也是相对安全的，以及由此产生的饲养细胞。 使用包含至少一种选自胎儿皮肤成纤维细胞，胎儿肌成纤维细胞，胎儿肺成纤维细胞，胎儿成纤维细胞，胎儿成纤维细胞，胎儿成纤维细胞，胎儿成纤维细胞 可以稳定地增殖上皮细胞，胎儿内皮细胞，成年皮肤成纤维细胞，成年肺成纤维细胞，成年上皮细胞和成为胚胎干细胞的饲养细胞的内皮细胞。