公开(公告)号：EP1489168A1

公开(公告)日：2004-12-22

申请号：EP02703964.3

申请日：2002-03-11

申请人： ReproCELL Inc. ,  Ema, Hideo

发明人： EMA, Hideo ,  NAKAUCHI, Hiromitsu ,  OSAWA, Mitsujiro

IPC分类号： C12N15/09 ,  C07K14/475 ,  C12N5/10 ,  A61K38/17 ,  A61P43/00 ,  A61K35/14 ,  A61K35/12

CPC分类号： C12N5/0647 ,  C07K14/47 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2501/415

摘要： The sustaining stem cells for example, hematopoietic stem cells while holding in the undifferentiated state and maintaining pluripotency by providing a polypeptide having a WIF domain for example, WIF-1 to the stem cells has been successfully achieved. This polypeptide preferably also contains an EGF-like repeat. This result has been achieved by providing a polypeptide preferably having the WIF domain together with a stem cell survival factor such as SCF. Thus, it becomes possible to easily provide a large amount of stem cells for example, hematopoietic stem cells containing functional precursor cells. It has also become possible to more efficiently transfer a gene into the stem cells. Because it is homogeneous and almost free from impurities, the stem cell composition can remarkably relieve side effects for example, infection, which occur in conventional therapies using hematopoietic stem cells.

摘要翻译： 维持性干细胞例如通过向干细胞提供具有WIF结构域（例如WIF-1）的多肽而保持未分化状态并保持多能性的造血干细胞已被成功地实现。 该多肽优选还含有EGF样重复。 通过提供优选将WIF结构域与干细胞存活因子如SCF一起提供的多肽来实现该结果。 因此，可以容易地提供大量的干细胞，例如含有功能性前体细胞的造血干细胞。 也可以更有效地将基因转移到干细胞中。 因为它是均质的并且几乎没有杂质，所以干细胞组合物可以显着地减轻使用造血干细胞的常规疗法中发生的副作用，例如感染。

公开(公告)号：EP1560916A1

公开(公告)日：2005-08-10

申请号：EP03758840.7

申请日：2003-10-23

申请人： ReproCELL Inc.

发明人： NAKAUCHI, Hiromitsu ,  IWAMA, Atsushi

IPC分类号： C12N5/06 ,  C07K14/47

CPC分类号： C12N5/0647 ,  C07K14/4705 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/26

摘要： The present invention relates to a method and substance for maintaining the pluripotency and self-replication ability of a stem cell, such as a hematopoietic stem cell, while keeping it undifferentiated. Specifically, the present invention provides a composition for maintaining the expansion or pluripotency of a stem cell, comprising active STAT5, and a method using the same. STAT5 may be in the form of a protein or a nucleic acid. The composition may contain a cellular physiologically active substance (e.g., SCF, TPO, Flt-3L, etc.). The present invention also relates to a cell, a tissue and an organ prepared from the stem cell.