公开(公告)号：EP3901268A1

公开(公告)日：2021-10-27

申请号：EP19900590.1

申请日：2019-12-19

申请人： Research Institute Of Innovative Technology For The Earth ,  Sumitomo Bakelite Co.Ltd.

发明人： INUI, Masayuki ,  HIRAGA, Kazumi ,  SUDA, Masako ,  HASHIZUME, Masayoshi ,  ISHIDA, Junya

IPC分类号： C12P7/22 ,  C12N1/21 ,  C12N15/60

摘要： Provided is a microorganism that is able to produce 2-phenylethanol at a high concentration, and a method of efficiently producing 2-phenylethanol by using a saccharide as a raw material.
Provided is a coryneform bacterium transformant in which a shikimate pathway is activated, and further, a gene that encodes an enzyme having phenylpyruvate decarboxylase activity is introduced in such a manner that the gene can be expressed.
Also provided is a 2-phenylethanol producing method that includes causing the coryneform bacterium transformant according to the present disclosure to react in water containing a saccharide.

公开(公告)号：EP3184626A1

公开(公告)日：2017-06-28

申请号：EP15834577.7

申请日：2015-08-20

申请人： Research Institute Of Innovative Technology For The Earth

发明人： INUI, Masayuki ,  SUDA, Masako ,  HIRAGA, Kazumi ,  KOGURE, Takahisa

IPC分类号： C12N1/21 ,  C12N15/09 ,  C12P7/42 ,  C12P13/22

摘要： A coryneform bacterium transformant engineered by the following (A) to (D): (A) enhancement of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase activity; (B) prevention, inhibition, or reduction of intracellular sugar uptake mediated by phosphotransferase system (PTS); (C) enhancement of intracellular sugar uptake activity mediated by a sugar transporter different from phosphotransferase system and enhancement of glucokinase activity; and (D) enhancement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity is capable of efficiently producing shikimic acid or the like from a sugar.

摘要翻译： （A） - （D）改造的棒状细菌转化体：（A）增强3-脱氧-D-阿拉伯 - 庚糖酸盐-7-磷酸（DAHP）合酶活性; （B）由磷酸转移酶系统（PTS）介导的细胞内糖摄取的预防，抑制或减少; （C）由不同于磷酸转移酶系统的糖转运蛋白介导的细胞内糖摄取活性的增强和葡糖激酶活性的增强; 和（D）甘油醛-3-磷酸脱氢酶（GAPDH）活性的增强能够有效地从糖生产莽草酸等。

公开(公告)号：EP3789481A1

公开(公告)日：2021-03-10

申请号：EP19796525.4

申请日：2019-02-18

申请人： Research Institute Of Innovative Technology For The Earth

发明人： INUI, Masayuki ,  HIRAGA, Kazumi ,  SUDA, Masako ,  KUBOTA, Takeshi

IPC分类号： C12N1/21 ,  C12N15/54 ,  C12N15/60 ,  C12P7/22 ,  C12N15/31

摘要： Provided is a transformant of a microorganism that has improved catechol productivity.
Provided in one aspect is a transformant of a coryneform bacterium that is obtained by introducing, into the coryneform bacterium as a host, a gene selected from the group consisting of
(1) a decarboxylase gene ubiD of Lactobacillus rhamnosus ;
(2) an ortholog of the gene (1) in at least one of the genus Lactobacillus, the genus Bacillus, the genus Enterobacter, the genus Escherichia, the genus Paenibacillus, the genus Citrobacter, or the genus Pantoea ; and
(3) a gene in which an enzyme that has an amino acid sequence identity of 70 % or more with an amino acid sequence of an enzyme encoded by the gene (1) or (2), and that has a decarboxylation activity, is encoded
Provided is a transformant of a coryneform bacterium wherein mutations are introduced into a catechol 1,2-dioxygenase gene catA, and a protocatechuic acid dehydrogenase gene pcaHG in the coryneform bacterium as a host; and functions of enzymes encoded by the two genes are degraded or lost.

公开(公告)号：EP3184626B1

公开(公告)日：2020-01-01

申请号：EP15834577.7

申请日：2015-08-20

申请人： Research Institute Of Innovative Technology For The Earth

发明人： INUI, Masayuki ,  SUDA, Masako ,  HIRAGA, Kazumi ,  KOGURE, Takahisa

IPC分类号： C12N9/12 ,  C12N15/77 ,  C12N15/87 ,  C12N15/09 ,  C12P7/42 ,  C12P13/22