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    TRANSFORMANTS CAPABLE OF PRODUCING ISOPROPANOL
    2.
    发明公开
    TRANSFORMANTS CAPABLE OF PRODUCING ISOPROPANOL 审中-公开
    ZUR HERSTELLUNG VON ISOPROPANOL GEEIGNETE TRANSFORMANTEN

    公开(公告)号：EP2182051A1

    公开(公告)日：2010-05-05

    申请号：EP08828643.0

    申请日：2008-08-28

    申请人： Research Institute Of Innovative Technology For The Earth ,  Honda Giken Kogyo Kabushiki Kaisha

    发明人： YUKAWA, Hideaki ,  INUI, Masayuki

    IPC分类号： C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  C12N15/09 ,  C12P7/04

    CPC分类号： C12N9/88 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/13 ,  C12P7/04

    摘要： A transformant capable of producing isopropanol which is constructed by transferring the following genes (a) to (d) into an aerobic bacterium or a facultative anaerobic bacterium:
    (a) a foreign gene which encodes an enzyme having acetyl-CoA acetyltransferase activity;
    (b) a foreign gene which encodes an enzyme having acetoacetyl CoA:acetate CoA-transferase activity;
    (c) a foreign gene which encodes an enzyme having acetoacetate decarboxylase activity; and
    (d) a foreign gene which encodes an enzyme having isopropanol dehydrogenase activity.

    摘要翻译： 能够生产异丙醇的转化体，其通过将以下基因（a）至（d）转移到需氧细菌或兼性厌氧细菌中而构建：（a）编码具有乙酰辅酶A乙酰转移酶活性的酶的外源基因; （b）编码具有乙酰乙酰CoA：乙酸CoA转移酶活性的酶的外源基因; （c）编码具有乙酰乙酸脱羧酶活性的酶的外源基因; 和（d）编码具有异丙醇脱氢酶活性的酶的外源基因。

    CORYNEFORM BACTERIUM TRANSFORMANT, AND PROCESS FOR PRODUCING ISOBUTANOL USING SAME
    4.
    发明公开
    CORYNEFORM BACTERIUM TRANSFORMANT, AND PROCESS FOR PRODUCING ISOBUTANOL USING SAME 审中-公开
    变革性CORYNEFORMER BAKTERIEN SOWIE VERFAHREN ZUR ISOBUTANOLHERSTELLUNG DAMIT

    公开(公告)号：EP2415860A1

    公开(公告)日：2012-02-08

    申请号：EP10758603.4

    申请日：2010-03-29

    申请人： Research Institute Of Innovative Technology For The Earth

    发明人： YUKAWA, Hideaki ,  INUI, Masayuki

    IPC分类号： C12N1/21 ,  C12N15/09 ,  C12P7/16

    CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/88 ,  Y02E50/10

    摘要： A Corynebacterium glutamicum transformant having the capability of producing isobutanol and the following genes (1) to (5):
    (1) a gene which encodes an enzyme having acetohydroxy acid synthase activity;
    (2) a gene which encodes an enzyme having acetohydroxy acid isomeroreductase activity;
    (3) a gene which encodes an enzyme having dihydroxy acid dehydratase activity;
    (4) a gene which encodes an enzyme having 2-keto acid decarboxylase activity; and
    (5) a gene which encodes an enzyme having alcohol dehydrogenase activity,

    at least one of the genes being endogenous, and at least one of the genes being exogenous, efficiently produces isobutanol.

    摘要翻译： 具有产生异丁醇能力的谷氨酸棒状杆菌转化体和以下基因（1）至（5）：（1）编码具有乙酰羟酸合成酶活性的酶的基因; （2）编码具有乙酰羟酸异构体还原酶活性的酶的基因; （3）编码具有二羟基酸脱水酶活性的酶的基因; （4）编码具有2-酮酸脱羧酶活性的酶的基因; 和（5）编码具有醇脱氢酶活性的酶的基因，至少一个基因是内源的，并且至少一个外源基因有效地产生异丁醇。

    GENETICALLY MODIFIED CORYNEFORM BACTERIA CAPABLE OF PRODUCING ISOPROPANOL
    6.
    发明公开
    GENETICALLY MODIFIED CORYNEFORM BACTERIA CAPABLE OF PRODUCING ISOPROPANOL 审中-公开
    巴基斯坦天然植物精油

    公开(公告)号：EP2270135A1

    公开(公告)日：2011-01-05

    申请号：EP09735982.2

    申请日：2009-04-15

    申请人： Research Institute Of Innovative Technology For The Earth

    发明人： YUKAWA, Hideaki ,  INUI, Masayuki

    IPC分类号： C12N1/21 ,  C12N15/09 ,  C12P7/04

    CPC分类号： C12N15/52 ,  C12P7/04 ,  C12R1/15

    摘要： A transformant capable of producing isopropanol which is constructed by transferring the following genes (a) to (d) into a coryneform bacterium:
    (a) an exogenous gene which encodes an enzyme having acetyl-CoA acetyltransferase activity;
    (b) an exogenous gene which encodes an enzyme having acetoacetyl CoA:acetate CoA-transferase activity;
    (c) an exogenous gene which encodes an enzyme having acetoacetate decarboxylase activity; and
    (d) an exogenous gene which encodes an enzyme having isopropanol dehydrogenase activity.

    摘要翻译： 能够产生异丙醇的转化体，其通过将以下基因（a）至（d）转移至棒状细菌构建：（a）编码具有乙酰辅酶A乙酰转移酶活性的酶的外源基因; （b）编码具有乙酰乙酰CoA：乙酸CoA转移酶活性的酶的外源基因; （c）编码具有乙酰乙酸脱羧酶活性的酶的外源基因; 和（d）编码具有异丙醇脱氢酶活性的酶的外源基因。

    DNA FRAGMENT HAVING PROMOTER FUNCTION
    7.
    发明公开
    DNA FRAGMENT HAVING PROMOTER FUNCTION 审中-公开
    DNA-FRAGMENT MIT PROMOTORFUNKTION

    公开(公告)号：EP1790721A1

    公开(公告)日：2007-05-30

    申请号：EP05776785.7

    申请日：2005-09-05

    申请人： RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH

    发明人： YUKAWA, Hideaki ,  INUI, Masayuki

    IPC分类号： C12N15/09 ,  C12N1/20

    CPC分类号： C07K14/34 ,  C12N15/67

    摘要： The present invention relates to a method of inducing expression of a promoter function of various genes in a Coryneform bacterium related to function exertion, in order to exert the function of a Coryneform bacterium highly and effectively under an anaerobic condition, for producing an organic compound useful under an anaerobic condition, more particularly, provides a method of enhancing and/or suppressing the promoter function related to various genes, for the purpose of highly and effectively expressing various protein genes necessary for production of an objective substance, and suppressing expression of an unnecessary protein gene.
    The DNA fragment of the present invention is useful as a primer which is introduced into a transformed Coryneform bacterium producing a useful substance such as lactic acid and succinic acid highly and at a high efficiency.

    摘要翻译： 本发明涉及在与功能运用相关的棒状细菌中诱导各种基因的启动子功能的表达的方法，以便在厌氧条件下高效且有效地发挥棒状细菌的功能，以产生有用的有机化合物 更具体地，提供了增强和/或抑制与各种基因相关的启动子功能的方法，目的是高度有效地表达生产目标物质所需的各种蛋白质基因，并抑制不必要的表达。 蛋白质基因。 本发明的DNA片段可用作引入转化棒杆菌型细菌的引物，其能够高效且高效地生产乳酸和琥珀酸等有用物质。

    TRANSFORMANT OF CORYNEFORM BACTERIUM AND PRODUCTION METHOD FOR USEFUL COMPOUND USING SAME
    8.
    发明公开
    TRANSFORMANT OF CORYNEFORM BACTERIUM AND PRODUCTION METHOD FOR USEFUL COMPOUND USING SAME 审中-公开

    公开(公告)号：EP3789481A1

    公开(公告)日：2021-03-10

    申请号：EP19796525.4

    申请日：2019-02-18

    申请人： Research Institute Of Innovative Technology For The Earth

    发明人： INUI, Masayuki ,  HIRAGA, Kazumi ,  SUDA, Masako ,  KUBOTA, Takeshi

    IPC分类号： C12N1/21 ,  C12N15/54 ,  C12N15/60 ,  C12P7/22 ,  C12N15/31

    摘要： Provided is a transformant of a microorganism that has improved catechol productivity.
    Provided in one aspect is a transformant of a coryneform bacterium that is obtained by introducing, into the coryneform bacterium as a host, a gene selected from the group consisting of
    (1) a decarboxylase gene ubiD of Lactobacillus rhamnosus ;
    (2) an ortholog of the gene (1) in at least one of the genus Lactobacillus, the genus Bacillus, the genus Enterobacter, the genus Escherichia, the genus Paenibacillus, the genus Citrobacter, or the genus Pantoea ; and
    (3) a gene in which an enzyme that has an amino acid sequence identity of 70 % or more with an amino acid sequence of an enzyme encoded by the gene (1) or (2), and that has a decarboxylation activity, is encoded
    Provided is a transformant of a coryneform bacterium wherein mutations are introduced into a catechol 1,2-dioxygenase gene catA, and a protocatechuic acid dehydrogenase gene pcaHG in the coryneform bacterium as a host; and functions of enzymes encoded by the two genes are degraded or lost.