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1.发明授权
公开(公告)号：EP0880536B1
公开(公告)日：2003-04-23
申请号：EP97901080.8
申请日：1997-01-24
发明人： KUHNE, Wolfgang
CPC分类号： A61K48/005 , A61K48/0091 , C12N15/101 , C12N15/85
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公开(公告)号：EP0973883A2
公开(公告)日：2000-01-26
申请号：EP98901955.9
申请日：1998-01-09
发明人： KUHNE, Wolfgang , POPP, Friedrich
CPC分类号： B01D21/262 , B01D2221/10 , C12N15/1003
摘要： The invention concerns a method of purifying extrachromosomal DNA by passing an extrachromosomal DNA and fluid containing further cell components through a cross-flow centrifuge under given conditions, resulting in separation of the extrachromosomal DNA from the other cell components such that purified extrachromosomal DNA is obtained. The invention further concerns the use of the purified extrachromosomal DNA for cloning, transformation, transfection and microinjection into cells, for use in gene therapy processes, DNA vaccination and/or for polymerase chain reaction (PCR). The invention finally concerns the use of a cross-flow centrifuge for purifying extrachromosomal DNA.
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3.发明授权
公开(公告)号：EP0880536B2
公开(公告)日：2015-08-05
申请号：EP97901080.8
申请日：1997-01-24
发明人： KUHNE, Wolfgang
CPC分类号： A61K48/005 , A61K48/0091 , C12N15/101 , C12N15/85
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4.发明公开REINIGUNG ODER/UND KONZENTRIERUNG VON DNA DURCH CROSS-FLOW-FILTRATION, ABTRENNUNG VON ENDOTOXINEN AUS EINER NUCLEINSÄURE-PRÄPARATION 失效清洁和/或DNA的浓度交叉流过滤，从核酸制备的内毒素的分离
公开(公告)号：EP0961826A2
公开(公告)日：1999-12-08
申请号：EP98904051.0
申请日：1998-01-09
发明人： KUHNE, Wolfgang
CPC分类号： C12N15/1017
摘要： The invention concerns a method of purifying and/or concentrating nucleic acids in a solution, the solution containing nucleic acid being guided tangentially past one or a plurality of semipermable membranes, such that the nucleic acid molecules are retained by the membranes and substances having a lower molecular weight can pass through the membranes and/or be adsorbed thereat, so that a purified and/or concentrated nucleic acid solution is obtained. The same method is carried out to separate endotoxins from a nucleic acid preparation. The invention further concerns the use of a cross-flow filtration system for purifying and/or concentrating nucleic acids in a solution and for separating endotoxins from a nucleic acid preparation. The invention also concerns the use of the nucleic acids purified and/or concentrated by the cross-flow filtration system for cloning, transformation, transfection and microinjection into cells, for use in gene therapy processes, DNA vaccination and/or for polymerase chain reaction (PCR).
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