公开(公告)号：EP0210279B1

公开(公告)日：1991-08-14

申请号：EP86900852.4

申请日：1986-01-24

申请人： SAGAMI CHEMICAL RESEARCH CENTER ,  CENTRAL GLASS COMPANY, LIMITED ,  HODOGAYA CHEMICAL COMPANY, LIMITED ,  NIPPON SODA CO., LTD. ,  NISSAN CHEMICAL INDUSTRIES LTD. ,  Tosoh Corporation

发明人： MIYAKE, Toshio ,  HIBINO, Yasuo ,  KOBAYASHI, Yoichi ,  WATABE, Ken ,  OMORI, Muneki ,  MIKI, Tetsuzo ,  YOKOYAMA, Midori ,  MATSUMOTO, Reiko ,  WATANABE, Kazuo ,  NUMAO, Naganori

IPC分类号： C12N9/72 ,  C12N15/58 ,  C12N1/21 ,  C12P21/02

CPC分类号： C12N9/6462 ,  C12N15/73 ,  C12Y304/21073

摘要： Stabilized human prourokinase-like polypeptides wherein the 135th lysine from the N-terminal of human prourokinase is substituted by an amino acid other than basic amino acids, the 157th phenylalanine is substituted by an acidic amino acid, or both of the amino acids are substituted as described above; DNA segment which codes said polypeptides; plasmid which contains said DNA segment; E. coli transformed by said plasmid; and a process for preparing said polypeptide by using said E. coli. The polypeptides are stable against proteases such as plasmin, thrombin, trypsin, etc., thus being easily purified and collected in a pure form. When applied to a living body, the polypeptides are expected to provide a persistent fibrinolytic activity.

公开(公告)号：EP0210279A1

公开(公告)日：1987-02-04

申请号：EP86900852.4

申请日：1986-01-24

申请人： SAGAMI CHEMICAL RESEARCH CENTER ,  CENTRAL GLASS COMPANY, LIMITED ,  HODOGAYA CHEMICAL COMPANY, LIMITED ,  NIPPON SODA CO., LTD. ,  NISSAN CHEMICAL INDUSTRIES LTD. ,  Tosoh Corporation

发明人： MIYAKE, Toshio ,  HIBINO, Yasuo ,  KOBAYASHI, Yoichi ,  WATABE, Ken ,  OMORI, Muneki ,  MIKI, Tetsuzo ,  YOKOYAMA, Midori ,  MATSUMOTO, Reiko ,  WATANABE, Kazuo ,  NUMAO, Naganori

IPC分类号： C12N9/72 ,  C12N15/58 ,  C12N1/21 ,  C12P21/02

CPC分类号： C12N9/6462 ,  C12N15/73 ,  C12Y304/21073

摘要： Stabilized human prourokinase-like polypeptides wherein the 135th lysine from the N-terminal of human prourokinase is substituted by an amino acid other than basic amino acids, the 157th phenylalanine is substituted by an acidic amino acid, or both of the amino acids are substituted as described above: DNA segment which codes said polypeptides; plasmid which contains said DNA sigment; E. coli transformed by said plasmid; and a process for preparing said polypeptide by using said E.coli. The polypeptides are stable against proteases such as plasmin, thrombin, trypsin. etc. thus being easily purified and collected in a pure form. When applied to a living body, the polypeptides are expected to provide a persistent fibrinolytic activity.

摘要翻译： 稳定的人类原尿激酶样多肽，其中人原尿激酶N末端的第135位赖氨酸被除碱性氨基酸以外的氨基酸取代，第157位苯丙氨酸被酸性氨基酸取代，或两者均被取代为 编码所述多肽的DNA片段; 含有所述DNA指征的质粒; 由所述质粒转化的大肠杆菌; 以及通过使用所述大肠杆菌制备所述多肽的方法。 多肽对蛋白酶如纤溶酶，凝血酶，胰蛋白酶是稳定的。 因此容易地以纯的形式纯化和收集。 当应用于活体时，期望多肽提供持久的纤维蛋白溶解活性。