公开(公告)号：EP0210279B1

公开(公告)日：1991-08-14

申请号：EP86900852.4

申请日：1986-01-24

申请人： SAGAMI CHEMICAL RESEARCH CENTER ,  CENTRAL GLASS COMPANY, LIMITED ,  HODOGAYA CHEMICAL COMPANY, LIMITED ,  NIPPON SODA CO., LTD. ,  NISSAN CHEMICAL INDUSTRIES LTD. ,  Tosoh Corporation

发明人： MIYAKE, Toshio ,  HIBINO, Yasuo ,  KOBAYASHI, Yoichi ,  WATABE, Ken ,  OMORI, Muneki ,  MIKI, Tetsuzo ,  YOKOYAMA, Midori ,  MATSUMOTO, Reiko ,  WATANABE, Kazuo ,  NUMAO, Naganori

IPC分类号： C12N9/72 ,  C12N15/58 ,  C12N1/21 ,  C12P21/02

CPC分类号： C12N9/6462 ,  C12N15/73 ,  C12Y304/21073

摘要： Stabilized human prourokinase-like polypeptides wherein the 135th lysine from the N-terminal of human prourokinase is substituted by an amino acid other than basic amino acids, the 157th phenylalanine is substituted by an acidic amino acid, or both of the amino acids are substituted as described above; DNA segment which codes said polypeptides; plasmid which contains said DNA segment; E. coli transformed by said plasmid; and a process for preparing said polypeptide by using said E. coli. The polypeptides are stable against proteases such as plasmin, thrombin, trypsin, etc., thus being easily purified and collected in a pure form. When applied to a living body, the polypeptides are expected to provide a persistent fibrinolytic activity.

公开(公告)号：EP0210279A1

公开(公告)日：1987-02-04

申请号：EP86900852.4

申请日：1986-01-24

申请人： SAGAMI CHEMICAL RESEARCH CENTER ,  CENTRAL GLASS COMPANY, LIMITED ,  HODOGAYA CHEMICAL COMPANY, LIMITED ,  NIPPON SODA CO., LTD. ,  NISSAN CHEMICAL INDUSTRIES LTD. ,  Tosoh Corporation

发明人： MIYAKE, Toshio ,  HIBINO, Yasuo ,  KOBAYASHI, Yoichi ,  WATABE, Ken ,  OMORI, Muneki ,  MIKI, Tetsuzo ,  YOKOYAMA, Midori ,  MATSUMOTO, Reiko ,  WATANABE, Kazuo ,  NUMAO, Naganori

IPC分类号： C12N9/72 ,  C12N15/58 ,  C12N1/21 ,  C12P21/02

CPC分类号： C12N9/6462 ,  C12N15/73 ,  C12Y304/21073

摘要： Stabilized human prourokinase-like polypeptides wherein the 135th lysine from the N-terminal of human prourokinase is substituted by an amino acid other than basic amino acids, the 157th phenylalanine is substituted by an acidic amino acid, or both of the amino acids are substituted as described above: DNA segment which codes said polypeptides; plasmid which contains said DNA sigment; E. coli transformed by said plasmid; and a process for preparing said polypeptide by using said E.coli. The polypeptides are stable against proteases such as plasmin, thrombin, trypsin. etc. thus being easily purified and collected in a pure form. When applied to a living body, the polypeptides are expected to provide a persistent fibrinolytic activity.

摘要翻译： 稳定的人类原尿激酶样多肽，其中人原尿激酶N末端的第135位赖氨酸被除碱性氨基酸以外的氨基酸取代，第157位苯丙氨酸被酸性氨基酸取代，或两者均被取代为 编码所述多肽的DNA片段; 含有所述DNA指征的质粒; 由所述质粒转化的大肠杆菌; 以及通过使用所述大肠杆菌制备所述多肽的方法。 多肽对蛋白酶如纤溶酶，凝血酶，胰蛋白酶是稳定的。 因此容易地以纯的形式纯化和收集。 当应用于活体时，期望多肽提供持久的纤维蛋白溶解活性。

公开(公告)号：EP0191869A1

公开(公告)日：1986-08-27

申请号：EP85904145.1

申请日：1985-08-16

申请人： HODOGAYA CHEMICAL COMPANY, LIMITED ,  Tosoh Corporation

发明人： OHMORI, Muneki ,  MIKI, Tetsuzo ,  NARUSHIMA, Hiroyuki ,  IKARI, Takaomi ,  SAITO, Akemi ,  IKUSHIMA, Noriko ,  MATSUMOTO, Reiko ,  WATANABE, Kazuo

IPC分类号： C12N15/00 ,  C12N1/20 ,  C07K7/36 ,  C12P21/02

CPC分类号： C12N9/0069 ,  A61K38/00 ,  C07K14/585 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/50 ,  C07K2319/75 ,  C12N15/62 ,  C12N15/70 ,  C12Y113/11002

摘要： This derivative comprises 33 amino acids wherein glycine is added to the C-terminal of fish calcitonin composed of 32 amino acids. This derivative itself has a biological activity and can be converted into natural fish calcitonin composed of 32 amino acids wherein protine in the C-terminal is amidated, by treating it in a conventional manner

摘要翻译： 该衍生物包含33个氨基酸，其中将甘氨酸加入由32个氨基酸组成的鱼降钙素的C末端。 该衍生物本身具有生物活性，可以转化成由32个氨基酸组成的天然鱼降钙素，其中C末端的脯氨酰胺化，通过以常规方式处理。

公开(公告)号：EP0969086A1

公开(公告)日：2000-01-05

申请号：EP97929493.1

申请日：1997-07-02

申请人： SAGAMI CHEMICAL RESEARCH CENTER ,  FUJIYAKUHIN COMPANY, LIMITED

发明人： YAZAWA, Kazunaga ,  WATANABE, Kazuo ,  ISHIKAWA, Chikako ,  TOMITA, Miho ,  MURAMATSU, Hiroshi

IPC分类号： C12N1/20 ,  C12P7/64

CPC分类号： C12P7/6427 ,  C12P7/6472 ,  C12R1/01

摘要： Microorganisms belonging to the genus Shewanella or Pseudoalteromonas which can produce docosahexaenoic acid (DHA) within a short culture period; a process for producing DHA which comprises culturing these microorganisms and isolating a lipid fraction from the culture; and a process for producing DHA labeled with a stable isotope with a high safety by using these microorganisms.

摘要翻译： 属于希瓦氏菌属或伪黄单胞菌属的微生物，其可以在短的培养期内产生二十二碳六烯酸（DHA）; 一种生产DHA的方法，包括培养这些微生物并从培养物中分离脂质部分; 以及通过使用这些微生物生产具有高安全性的稳定同位素标记的DHA的方法。