公开(公告)号：EP1651660A4

公开(公告)日：2012-05-23

申请号：EP04780272

申请日：2004-08-06

申请人： SANGAMO BIOSCIENCES INC

发明人： PABO CARL O ,  URNOV FYODOR ,  HOLMES MICHAEL C ,  MILLER JEFFREY C ,  GUSCHIN DMITRY

IPC分类号： C12N9/22 ,  C07K14/47 ,  C12N20060101 ,  C12N15/01 ,  C12N15/10 ,  C12N15/62

CPC分类号： C12N15/10 ,  C07K14/4702 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/902

摘要： Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to M the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.

摘要翻译： 本文公开了用于基因组序列的靶向切割，基因组序列的靶向改变以及基因组区域和与该基因组区域同源的外源多核苷酸之间的靶向重组的方法和组合物。 组合物包括包含切割结构域（或切割半结构域）和工程化锌指结构域的融合蛋白和编码它们的多核苷酸。 用于靶向切割的方法包括将这种融合蛋白或编码其的多核苷酸引入细胞。 靶向重组的方法还包括将与基因组区域同源的外源多核苷酸引入包含所公开的融合蛋白的细胞中。