公开(公告)号：EP1545535A4

公开(公告)日：2008-06-04

申请号：EP03749389

申请日：2003-09-05

申请人： SCIOS INC ,  UNIV CALIFORNIA

发明人： PROTTER ANDREW ASHER ,  SVENSSON CAMILLA ,  YAKSH TONY ,  CORDELL BARBARA ,  DUGAR SUNDEEP

IPC分类号： A61K31/381 ,  A61K31/4164 ,  A61K31/44 ,  A61K31/496 ,  A61K31/505 ,  A61K31/17 ,  A61K31/454

CPC分类号： A61K31/496 ,  A61K31/381 ,  A61K31/4164 ,  A61K31/44

公开(公告)号：EP1176871A4

公开(公告)日：2004-03-17

申请号：EP00932358

申请日：2000-05-11

申请人： SCIOS INC ,  LILLY CO ELI

发明人： ZHONG ZIYANG ,  CORDELL BARBARA ,  QUON DIANA HOM ,  LIU YU-WANG ,  XU QIANG ,  JOHNSTONE EDWARD MARION ,  HYSLOP PAUL ANDREW ,  SCHIMMOELLER FRAUKE ,  LITTLE SHEILA PARKS ,  QUEENER STEPHEN WYATT ,  YIN TINGGUI

IPC分类号： G01N33/50 ,  A61K38/00 ,  A61K45/00 ,  A61P25/28 ,  A61P43/00 ,  C07B61/00 ,  C12N9/64 ,  C12N9/99 ,  C12Q1/02 ,  C12Q1/37 ,  C12R1/91 ,  G01N33/15 ,  G01N33/53

CPC分类号： C12N9/6478 ,  A61K38/00 ,  C07K14/4711 ,  C12N9/6421 ,  C12Q1/37 ,  G01N33/6896 ,  G01N2500/00

摘要： The invention concerns a novel β-secretase, a method of partially purifying this novel β-secretase, and its use in assays to screen for potential drug candidates against Alzheimer's disease and other neurological diseases. The novel β-secretase has an estimated molecular weight of about 32-39 kDa or 22-26 kDa in HEK293 cell membrane extracts and human brain samples, respectively, as calculated from radiation inactivation analysis, and has a pH optimum at about pH 6.5-7.0.