公开(公告)号：EP0832096A4

公开(公告)日：1998-08-05

申请号：EP95919029

申请日：1995-05-04

申请人： SCRIPPS RESEARCH INST

发明人： KENT STEPHEN B H ,  MUIR TOM W ,  DAWSON PHILIP E

IPC分类号： C07K1/02 ,  C07K1/04 ,  C07K1/08 ,  C07K1/13 ,  C07K17/06 ,  C08G69/10 ,  C08L89/00

CPC分类号： C07K1/04 ,  C07K1/026

摘要： Proteins of moderate size having native peptide backbones are produced by a method of native chemical ligation. Native chemical ligation employs a chemoselective reaction of two unprotected peptide segments to produce a transient thioester-linked intermediate. The transient thioester-linked intermediate then spontaneously undergoes a rearrangement to provide the full length ligation product having a native peptide bond at the ligation site. Full length ligation products are chemically identical to proteins produced by cell free synthesis. Full length ligation products may be refolded and/or oxidized, as allowed, to form native disulfide-containing protein molecules. The technique of native chemical ligation is employable for chemically synthesizing full length proteins.

公开(公告)号：EP0635025A4

公开(公告)日：1997-02-26

申请号：EP93909180

申请日：1993-03-26

申请人： SCRIPPS RESEARCH INST

发明人： KENT STEPHEN B H ,  SCHNOLZER-RACKWITZ MARTINA

IPC分类号： C07K1/04 ,  C07K1/06 ,  C07K1/10 ,  C07K1/107 ,  C07K1/113 ,  C07K7/02 ,  C07K14/16 ,  C12N9/50 ,  C07K3/04

CPC分类号： C07K14/005 ,  C07K1/04 ,  C07K1/1075 ,  C07K1/1077 ,  C07K7/02 ,  C12N9/506 ,  C12N2740/16222 ,  Y02P20/582

摘要： Process for the preparation of modified proteins comprising the coupling of a first peptide segment having a haloacyl group at the N-terminus thereof with a second peptide segment having a carbonylthiol group at the C-terminus thereof are disclosed. Novel modified proteins produced by the process are also disclosed.

公开(公告)号：EP0804215A4

公开(公告)日：1998-07-01

申请号：EP94925866

申请日：1994-08-11

申请人： SCRIPPS RESEARCH INST

发明人： DAWSON PHILIP E ,  KENT STEPHEN B H

IPC分类号： C07K1/107 ,  C07K7/02 ,  C07K7/06 ,  C07K7/08 ,  C07K19/00 ,  A61K38/00

CPC分类号： C07K7/02 ,  C07K1/1077

摘要： A chemoselective ligation approach to the preparation of complex peptides is employed in a simple, convenient synthesis of template-assembled synthetic protein (TASP) molecules. Reaction of readily prepared synthetic pro-helical peptide- alpha COSH molecules, in unprotected form, with a synthetic (BrAc)4template peptide molecule, also in unprotected form, proceeds rapidly in aqueous solution to give a uniform product in high yield. The resulting TASP molecule may be simply purified to homogeneity. Structural homogeneity may be demonstrated by ionspray mass spectrometry. The chemoselective ligation of unprotected peptides represents a general approach to the synthesis of TASP molecules.