公开(公告)号：EP0816380A4

公开(公告)日：1998-11-18

申请号：EP96902484

申请日：1996-02-20

申请人： SNOW BRAND MILK PROD CO LTD

发明人： GOTO MASAAKI ,  TSUDA EISUKE ,  MOCHIZUKI SHIN ICHI ,  YANO KAZUKI ,  KOBAYASHI FUMIE ,  SHIMA NOBUYUKI ,  YASUDA HISATAKA ,  NAKAGAWA NOBUAKI ,  MORINAGA TOMONORI ,  UEDA MASATSUGU ,  HIGASHIO KANJI

IPC分类号： C07K14/52 ,  A61K38/19 ,  A61P19/10 ,  C07K14/705 ,  C07K16/24 ,  C12N1/21 ,  C12N5/08 ,  C12N15/12 ,  C12N15/19 ,  C12P21/02 ,  C12P21/08 ,  C12N5/10 ,  C12N5/20 ,  C12N15/06 ,  G01N33/577

CPC分类号： C07K14/70578

摘要： A novel protein having the activity of suppressing the differentiation and/or maturation of osteoclasts and methods of the production of the same. This protein is produced from human fetal pulmonary fibroblasts and has a molecular weight of about 60 KD under reductive conditions or about 120 KD under nonreductive conditions. It can be isolated and purified from the culture medium of the above-mentioned cells. Alternatively, it can be produced by genetic engineering techniques. The invention also provides a cDNA for the genetic engineering production of the protein, an antibody showing an affinity specifically for the protein, and a method for assaying the protein with the use of this antibody.

摘要翻译： 抑制破骨细胞分化和/或成熟的蛋白质和蛋白质的生产方法。 蛋白质由人类胚胎肺成纤维细胞产生，在非还原条件下分子量分别为约60kD和约120kD，还原条件下SDS-聚丙烯酰胺凝胶电泳分子量分别为约60kD。 可以从所述成纤维细胞的培养基中分离和纯化蛋白质。 此外，蛋白质可以通过基因工程产生。 本发明包括通过基因工程产生蛋白质的cDNA，对蛋白质具有特异性亲和力的抗体或使用该抗体测定蛋白质浓度的方法。