公开(公告)号：EP0865486A4

公开(公告)日：2002-06-26

申请号：EP96937005

申请日：1996-10-24

申请人： SQUIBB BRISTOL MYERS CO

发明人： BURTON STEVEN J ,  PEARSON JAMES C ,  EDWARDSON PETER A D ,  MENZIES ALAN

IPC分类号： G01N33/53 ,  A61L2/00 ,  C07D495/04 ,  C07K14/75 ,  C07K17/06 ,  C12N9/00 ,  C12N11/06 ,  C12P21/00 ,  G01N33/531 ,  G01N33/543 ,  C12N11/00 ,  C12N11/02 ,  C12N11/16 ,  C12P21/06

CPC分类号： G01N33/54306 ,  A61L2/0035 ,  C07K14/75

摘要： Compositions and methods for biotinylated biomolecules, such as enzymes, are disclosed. The compositions include a biotinylated biomolecule, a biomolecule protectant, a buffer, a bulking agent selected from one or more water soluble, nonionic polymers and preferably a terminal sterilization protectant. The compositions can be utilized either as aqueous solutions or preferably in dried form, e.g., as a lyophilized powder cake. They have applicability in any case wherein avidin/biotin technology is used, and are particularly important as compositions containing a thrombin-like enzyme. The preparation of a fibrin monomer and fibrin monomer-based fibrin sealants are an example wherein avidin/biotin technology is used and methods of providing such monomers are also disclosed.

摘要翻译： 提供含有生物素化分子如酶的稳定组合物。 组合物包括生物素化的生物分子，生物分子保护剂，缓冲剂，选自一种或多种水溶性非离子聚合物的填充剂，优选末端灭菌保护剂。 组合物可以作为水溶液或优选以干燥形式使用，例如作为冻干的粉饼。 它们在使用抗生物素蛋白/生物素技术的任何情况下都适用，并且作为含有凝血酶样酶的组合物是特别重要的，例如用于制备纤维蛋白单体和基于纤维蛋白单体的纤维蛋白密封剂。

公开(公告)号：EP0902834A4

公开(公告)日：2001-09-26

申请号：EP96937780

申请日：1996-10-28

申请人： SQUIBB BRISTOL MYERS CO

发明人： BURTON STEVEN J ,  PEARSON JAMES C ,  EDWARDSON PETER A D

IPC分类号： C12N11/10 ,  A61L2/00 ,  A61L24/10 ,  C07K1/22 ,  C07K14/36 ,  C07K14/465 ,  C07K14/75 ,  C07K17/10 ,  C12N11/16 ,  C12P21/06 ,  C12N11/00 ,  C12N11/02 ,  G01N33/53

CPC分类号： C07K14/36 ,  A61L2/0035 ,  A61L24/106 ,  C07K1/22 ,  C07K14/465 ,  C07K14/75 ,  C07K17/10 ,  C12P21/06 ,  Y10S435/966 ,  Y10S435/967 ,  Y10S435/972 ,  Y10S530/81 ,  Y10S530/812 ,  Y10S530/813

摘要： Compositions and methods for avidin immobilized on an inert support material, e.g., agarose, are disclosed. The compositions have high activity levels of avidin and may further include a bulking agent, e.g., maltose, and a protectant to maintain the stability and integrity of the avidin agarose during lyophilization and terminal sterilization processes. These compositions have applicability in any instance wherein avidin, agarose and/or the avidin/biotin technology are useful. In particular, the present compositions are useful in an enzyme capture system to prepare fibrin monomer useful for fibrin sealants.