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1.发明公开Immobilized lactase, its preparation and use 失效Immobilisierte Laktase，ihre Herstellung und Verwendung。
公开(公告)号：EP0037667A2
公开(公告)日：1981-10-14
申请号：EP81301213.5
申请日：1981-03-20
发明人： Hirohara, Hideo , Yamamoto, Hidefumi , Kawano, Emiko , Nabeshima, Shigeyasu , Mitsuda, Satoshi , Nagase, Tsuneyuki
CPC分类号： A23C9/1206 , A23C2220/104 , C12N11/08
摘要： A stable and water-insoluble immobilized lactase having a high lactose-hydrolyzing activity is produced with industrial advantages by immobilizing a lactase derived from Aspergillus oryzae on a macroporous phenol-formaldehyde anion-exchange resin. The immobilized lactase can be used in the continuous enzymatic hydrolysis of lactose in milk, whey and whey permeate.
摘要翻译： 通过将来自米曲霉的乳糖酶固定在大孔酚醛阴离子交换树脂上，产生具有高乳糖水解活性的稳定的水不溶性固定化乳糖酶，具有工业优点。 固定化的乳糖酶可用于牛奶，乳清和乳清混合物中乳糖的连续酶水解。
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公开(公告)号：EP0037667B1
公开(公告)日：1984-06-27
申请号：EP81301213.5
申请日：1981-03-20
发明人： Hirohara, Hideo , Yamamoto, Hidefumi , Kawano, Emiko , Nabeshima, Shigeyasu , Mitsuda, Satoshi , Nagase, Tsuneyuki
CPC分类号： A23C9/1206 , A23C2220/104 , C12N11/08
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公开(公告)号：EP0026672B1
公开(公告)日：1985-06-05
申请号：EP80303452.9
申请日：1980-09-30
发明人： Hirohara, Hideo , Yamamoto, Hidefumi , Kawano, Emiko , Nabeshima, Shigeyasu , Mitsuda, Satoshi , Nagase, Tsuneyuki
CPC分类号： A23C9/1206 , A23C21/023 , A23C2220/104 , C12N9/2402 , C12N9/2471 , C12N9/86 , C12N11/08 , C12Y302/01023 , C12Y302/01108 , C12Y305/02006
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4.发明公开
公开(公告)号：EP0026672A3
公开(公告)日：1981-04-22
申请号：EP80303452
申请日：1980-09-30
发明人： Hirohara, Hideo , Yamamoto, Hidefumi , Kawano, Emiko , Nabeshima, Shigeyasu , Mitsuda, Satoshi , Nagase, Tsuneyuki
CPC分类号： A23C9/1206 , A23C21/023 , A23C2220/104 , C12N9/2402 , C12N9/2471 , C12N9/86 , C12N11/08 , C12Y302/01023 , C12Y302/01108 , C12Y305/02006
摘要： Previously, no immobilised lactase was both stable and highly active to lactose. This problem is now overcome with a stable and water-insoluble immobilized lactase prepared by immobilizing a lactase derived from Aspergillus oryzae on a macroporous amphoteric phenolformaldehyde type ion-exchange resin. The immobilized lactase can be employed in the continuous hydrolysis of lactose in milk and whey.
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5.发明公开Immobilized lactase and its use in hydrolysing lactose 失效Immobilisierte Laktase und deren Verwendung zur Hydrolyse von Laktose。
公开(公告)号：EP0026672A2
公开(公告)日：1981-04-08
申请号：EP80303452.9
申请日：1980-09-30
发明人： Hirohara, Hideo , Yamamoto, Hidefumi , Kawano, Emiko , Nabeshima, Shigeyasu , Mitsuda, Satoshi , Nagase, Tsuneyuki
CPC分类号： A23C9/1206 , A23C21/023 , A23C2220/104 , C12N9/2402 , C12N9/2471 , C12N9/86 , C12N11/08 , C12Y302/01023 , C12Y302/01108 , C12Y305/02006
摘要： Previously, no immobilised lactase was both stable and highly active to lactose. This problem is now overcome with a stable and water-insoluble immobilized lactase prepared by immobilizing a lactase derived from Aspergillus oryzae on a macroporous amphoteric phenolformaldehyde type ion-exchange resin. The immobilized lactase can be employed in the continuous hydrolysis of lactose in milk and whey.
摘要翻译： 以前，没有固定化的乳糖酶对乳糖稳定和高度活性。 现在通过将来自米曲霉的乳糖酶固定在大孔两性苯酚甲醛型离子交换树脂上制备的稳定且不溶于水的固定化乳糖酶来克服该问题。 固定化的乳糖酶可用于乳和乳清中乳糖的连续水解。
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公开(公告)号：EP0037667A3
公开(公告)日：1981-12-02
申请号：EP81301213
申请日：1981-03-20
发明人： Hirohara, Hideo , Yamamoto, Hidefumi , Kawano, Emiko , Nabeshima, Shigeyasu , Mitsuda, Satoshi , Nagase, Tsuneyuki
CPC分类号： A23C9/1206 , A23C2220/104 , C12N11/08
摘要： A stable and water-insoluble immobilized lactase having a high lactose-hydrolyzing activity is produced with industrial advantages by immobilizing a lactase derived from Aspergillus oryzae on a macroporous phenol-formaldehyde anion-exchange resin. The immobilized lactase can be used in the continuous enzymatic hydrolysis of lactose in milk, whey and whey permeate.
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