公开(公告)号：EP1926832A1

公开(公告)日：2008-06-04

申请号：EP06796654.9

申请日：2006-08-16

申请人： SUNTORY LIMITED

发明人： KODAMA, Yukiko ,  HATANAKA, Kazushige ,  TANAKA, Koichi ,  NAKAGAWA, Hiroko ,  MORIYA, Shogo ,  OSANO, Kaoru

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6895 ,  C12Q1/6888

摘要： The present invention relates to an instrument, a method and a kit for detecting a microorganism contaminating a subject test sample, which enables one to quickly and accurately identify the microorganism with an easy operation. The instrument for detecting a microorganism according to the present invention relates to a microarray type instrument in which oligonucleotides prepared based on nucleotide sequences specific to the species and genus to which the subject microorganism belongs have been immobilized onto a surface of a substrate. Based on the presence or absence of hybridization of the probes prepared from the test sample with the oligonucleotides immobilized onto the surface of the substrate, the present invention makes it possible to detect and/or identify the microorganism in the test sample easily, quickly and accurately.

公开(公告)号：EP1788093A1

公开(公告)日：2007-05-23

申请号：EP05780216.7

申请日：2005-08-04

申请人： SUNTORY LIMITED ,  Nisshinbo Industries, Inc.

发明人： YAMANAKA, Mikiko ,  MORIYA, Shougo ,  OSANO, Kaoru ,  OKEGAWA, Takashi

IPC分类号： C12Q1/68 ,  C12N15/00 ,  C12M1/34 ,  C12Q1/04

CPC分类号： C12Q1/689

摘要： The present invention provides a bacteria detection device, bacteria detection method and bacteria detection kit enabling rapid and accurate determination of bacteria present in a test sample, and particularly spore-forming, aerobic bacteria, with a simple procedure. The bacteria detection device according to the present invention is a microarray type of device in which an oligonucleotide, which is based on a nucleotide sequence specific to a genus or species to which a target spore-forming, aerobic bacteria belongs, is immobilized on a substrate. Spore-forming, aerobic bacteria present in a test sample can be easily, rapidly and accurately detected and identified based on the presence or absence of successful hybridization between a probe prepared from the test sample and the oligonucleotide immobilized on the substrate.

摘要翻译： 本发明提供了一种细菌检测装置，细菌检测方法和细菌检测试剂盒，其能够以简单的方法快速且准确地测定存在于测试样品中的细菌，特别是孢子形成的需氧细菌。 本发明的细菌检测装置是将基于特定于目标孢子形成好氧细菌所属的属或种的核苷酸序列的寡核苷酸固定在基板上的微阵列型装置 。 基于从测试样品制备的探针和固定在底物上的寡核苷酸之间存在或不存在成功的杂交，可以容易，快速和准确地检测和鉴定存在于测试样品中的孢子形成，需氧细菌。

公开(公告)号：EP1726662A1

公开(公告)日：2006-11-29

申请号：EP05710702.1

申请日：2005-02-25

申请人： SUNTORY LIMITED ,  Nisshinbo Industries, Inc.

发明人： KODAMA, Yukiko ,  HATANAKA, Kazushige ,  TANAKA, Koichi ,  NAKAGAWA, Hiroko ,  MORIYA, Shougo ,  OSANO, Kaoru

IPC分类号： C12Q1/68 ,  C12N15/09 ,  C12M1/34

CPC分类号： C12Q1/689 ,  C12Q1/6837

摘要： It is intended to provide an instrument for detecting bacteria by which bacteria contaminating a test sample can be quickly and exactly determined by a convenient procedure, a method of detecting bacteria and a kit for detecting bacteria.
Namely, a microarray type instrument wherein an oligonucleotide based on a base sequence specific to the genus or species, to which the target bacteria belong, is immobilized on the surface of a substrate. The target bacteria in the test sample can be conveniently, quickly and exactly detected and identified depending on the occurance or non-occurance of the hybridization between a probe prepared from the test sample and the oligonucleotide immobilized on the surface of the substrate as described above.

摘要翻译： 它的目的是用于检测细菌，通过该细菌污染的测试样品可以是快速，准确地确定由一个方便的程序，检测细菌的方法，以及用于检测细菌的试剂盒提供开采仪器。 即，基于特定于属或种的碱基序列，与靶细菌属于哪个寡核苷酸的微阵列型器具worin，被固定在基板的表面上。 测试样品中靶细菌可以方便，快速，准确地检测和识别取决于一次出现或从测试样品制备的探针和固定在基板如上述那样的表面上的寡核苷酸之间的杂交的非一次出现。