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16 条记录 (耗时 0.01 秒)

    Process for production of protein
    1.
    发明公开
    Process for production of protein 失效
    一种用于生产蛋白质的过程

    公开(公告)号：EP0700995A3

    公开(公告)日：1996-10-16

    申请号：EP95306235.3

    申请日：1995-09-06

    申请人： SUNTORY LIMITED

    发明人： Yabuta, Masayuki ,  Ohsuye, Kazuhiro

    IPC分类号： C12N15/09 ,  C12N15/66

    CPC分类号： C12N9/52 ,  C07K2319/00 ,  C12N15/62

    摘要： A process for the production of a desired polypeptide comprising the steps of: (1) transforming host cells with an expression vector comprising a gene coding for a fusion protein comprising a desired polypeptide and a protective polypeptide; (2) culturing the transformed host cells so as to express said gene to produce a fusion protein; and (3) excising the desired polypeptide from the fusion protein with a protease intrinsic to the host cells. A large amount of a desired polypeptide can be produced at a low cost. Especially according to the present invention, a large amount of S. aureus V8 protease can be efficiently produced at low cost using a safe host as E. coli according to gene recombination procedures.

    Mutant staphylococcus aureus V8 proteases
    3.
    发明公开
    Mutant staphylococcus aureus V8 proteases 失效
    金黄色葡萄球菌V8蛋白酶突变体

    公开(公告)号：EP0745669A3

    公开(公告)日：1999-05-26

    申请号：EP96303939.1

    申请日：1996-05-31

    申请人： SUNTORY LIMITED

    发明人： Yabuta, Masayuki ,  Ohsuye, Kazuhiro

    IPC分类号： C12N9/00

    CPC分类号： C07K14/58 ,  C07K2319/00 ,  C12N9/52

    摘要： Mutant proteases are obtained with one or more mutation sites in the natural V8 protease protein, and with enzyme activities even in the presence of high urea concentrations. Inactivation of enzyme activity is minimized even in the presence of high concentrations of urea, to thus allow lower amounts of enzyme to be added to urea-containing reaction systems and shorten reaction times. As an additional advantage, the ability to cleave proteins in the presence of high urea concentrations makes it possible to obtain hitherto unobtainable peptide fragments.

    Method for controlling gene expression
    4.
    发明公开
    Method for controlling gene expression 失效
    控制基因表达的方法。

    公开(公告)号：EP0685560A3

    公开(公告)日：1998-01-07

    申请号：EP95303708.2

    申请日：1995-05-31

    申请人： SUNTORY LIMITED

    发明人： Yabuta, Masayuki ,  Miura, Seiko ,  Ohsuye, Kazuhiro

    IPC分类号： C12N15/72 ,  C07K14/245 ,  C12N15/31

    CPC分类号： C12N15/72 ,  C07K14/245

    摘要： In a lactose repressor protein, at least one amino acid at the position of 94, 241, 265 or 300 in the wild lactose repressor is replaced with an amino acid other than that of the wild lactose repressor, and the use thereof. The present mutant lactose repressor represses the expression of a desired gene at 30°C or lower temperature, and induces the expression of a desired gene at 37°C or higher temperature, and therefor can control the expression by change of a culture temperature without using an expensive inducer such as IPTG.

    Process for production of protein
    5.
    发明公开
    Process for production of protein 失效
    Verfahren zur Herstellung von Proteinen。

    公开(公告)号：EP0649900A2

    公开(公告)日：1995-04-26

    申请号：EP94306587.0

    申请日：1994-09-07

    申请人： SUNTORY LIMITED

    发明人： Furukawa, Kazuaki ,  Sugimura, Keijiro ,  Ohsuye, Kazuhiro

    IPC分类号： C12N1/38 ,  C12P21/02

    CPC分类号： C12N9/0071 ,  C07K14/57 ,  C12N1/38 ,  C12P21/02

    摘要： A process for production of a desired protein comprising the steps of:
       a culturing animal cells capable of producing the desired protein in a medium containing trichostatin compounds; and
       recovering the desired protein from the culture.

    摘要翻译： 一种生产所需蛋白质的方法，包括以下步骤：培养能够在含有曲古抑菌素化合物的培养基中产生所需蛋白质的动物细胞; 并从培养物中回收所需的蛋白质。

    Methods for animal cell culture
    7.
    发明公开
    Methods for animal cell culture 失效
    Methoden zurZüchtungvon Tierzellen

    公开(公告)号：EP0764719A2

    公开(公告)日：1997-03-26

    申请号：EP96306828.3

    申请日：1996-09-19

    申请人： SUNTORY LIMITED

    发明人： Furukawa, Kazuaki ,  Ohsuye, Kazuhiro

    IPC分类号： C12N5/00 ,  C12P21/00 ,  C12N9/02 ,  C12N15/53

    CPC分类号： C12N9/0071 ,  C12N5/00 ,  C12N2523/00 ,  C12P21/02

    摘要： Methods for improving productivity in the production of useful substances by animal cells. A method for animal cell culture to produce a desired substance comprises the steps of (1) culturing animal cells at a temperature at which the animal cells can grow; and (2) culturing the animal cells at a lower temperature.

    摘要翻译： 提高动物细胞生产有用物质生产力的方法。 用于产生所需物质的动物细胞培养的方法包括以下步骤：（1）在动物细胞生长的温度下培养动物细胞; 和（2）在较低温度培养动物细胞。

    Process for producing peptides
    8.
    发明公开
    Process for producing peptides 失效
    生产肽的方法

    公开(公告)号：EP0528686A3

    公开(公告)日：1994-04-06

    申请号：EP92307577.4

    申请日：1992-08-12

    申请人： SUNTORY LIMITED

    发明人： Yabuta, Masayuki ,  Suzuki, Yuji ,  Ohsuye, Kazuhiro ,  Oshima, Takehiro ,  Onai, Seiko ,  Magota, Koji ,  Tanaka, Shoji

    IPC分类号： C12N15/62 ,  C12P21/02 ,  C12N15/16

    CPC分类号： C07K14/58 ,  C07K14/585 ,  C07K2319/00 ,  C07K2319/50 ,  C07K2319/75 ,  C12N9/2471 ,  C12N15/62 ,  C12Y302/01023

    摘要： A process for production of a peptide (target peptide) comprising:
    A) culturing host cells transformed with a plasmid able to express a gene coding for a fusion protein represented in the following formula:
            A-L-B
    wherein, B is a target peptide, A is a protective peptide comprised of 90-200 amino acid residues, and L is a linker peptide positioned between the C-terminal of said protective peptide and the N-terminal of said target peptide and selected so that said fusion protein is treated by an enzyme or chemical substance, the above-mentioned target peptide is separated, and said protective peptide and linker peptide are selected so that the isoelectric point of the entire fusion protein A-L-B is within a range of 4.9-6.9; B) obtaining an insoluble fraction comprising inclusion bodies by homogenization of the cultured cells of said transformant body; C) solubilizing of a fusion protein in said inclusion bodies by treatment of said insoluble fraction with solubilizing agent; and, D) cleaving of the peptide bond between the C-terminal of the linker amino acid residue and the N-terminal of the target peptide of said solubilized fusion protein to release said target peptide from the other peptides followed by purification of said target peptide. The present invention is able to express a target peptide in a large amount and accumulate said target peptide in host cells in the form of inclusion bodies.

    摘要翻译： 一种生产肽（目标肽）的方法，包括：A）培养用能够表达编码下式所示的融合蛋白的基因的质粒转化的宿主细胞：ALB其中，B是目标肽，A是 由90-200个氨基酸残基组成的保护肽，并且L是位于所述保护肽的C末端和所述目标肽的N末端之间的接头肽，并且被选择为使得所述融合蛋白被酶或化学品 物质时，分离上述目标肽，并选择所述保护肽和接头肽，使得整个融合蛋白ALB的等电点在4.9-6.9的范围内; B）通过均质化所述转化体的培养细胞获得包含包涵体的不溶性部分; C）通过用增溶剂处理所述不溶性部分来溶解所述包涵体中的融合蛋白; 和D）切割接头氨基酸残基的C末端和所述可溶化融合蛋白的目标肽的N末端之间的肽键以从其他肽释放所述目标肽，接着纯化所述目标肽 。 本发明能够大量表达目标肽并以包涵体的形式在宿主细胞中积累所述目标肽。