公开(公告)号：EP2022861A1

公开(公告)日：2009-02-11

申请号：EP07743388.6

申请日：2007-05-15

申请人： Sapporo Medical University ,  St. Marianna University School of Medicine

发明人： WATANABE, Yoshiyuki ,  TOYOTA, Minoru ,  IMAI, Kohzo ,  SHINOMURA, Yasuhisa ,  ITOH, Fumio ,  TOKINO, Takashi

IPC分类号： C12Q1/68 ,  C12M1/00 ,  C12M1/26 ,  C12M3/00 ,  G01N33/15 ,  C12N15/09

CPC分类号： C12Q1/6806

摘要： The invention relates to a sample containing a gastricmucosa lavage fluid collected from a subject who has received a gastric mucus removal treatment and a method for detecting a disease-related marker using the same. By using the sample of the invention, the disease-related marker can be detected conveniently, less invasively, highly sensitively and highly accurately.

摘要翻译： 本发明涉及一种含有从谁收到了胃粘液除去处理和用于检测使用其的疾病相关标记物的方法的受试者收集的gastricmucosa灌洗液样品。 通过使用本发明的样品，所述疾病相关标记物可以方便地，较少侵入性，高度灵敏地检测和高度精确地设定。

公开(公告)号：EP2295598A1

公开(公告)日：2011-03-16

申请号：EP09742626.6

申请日：2009-05-07

申请人： Sapporo Medical University

发明人： SUZUKI, Hiromu ,  TOYOTA, Minoru ,  IMAI, Kohzoh ,  SHINOMURA, Yasuhisa ,  TOKINO, Takashi

IPC分类号： C12Q1/68 ,  A61K31/7105 ,  A61K31/711 ,  A61K48/00 ,  A61P35/00 ,  C12N15/09 ,  C07K14/47

CPC分类号： C12Q1/6886 ,  A61K31/7105 ,  A61K31/711 ,  C07K14/47 ,  C12N15/113 ,  C12N2310/141 ,  C12N2320/10 ,  C12N2330/10 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/178

摘要： The object aims to comprehensively analyze miRNA that undergoes epigenetic silencing in cancer to identify miRNA associated with cancer, elucidate the role of the identified miRNA in cancer, and develop a novel method for detecting cancer and a novel therapeutic agent for cancer both of which relate to the miRNA. Disclosed is a method for detecting cancer in a subject, which comprises detecting methylated CpG in a CpG island located in a promoter region of a microRNA 34b gene and/or a microRNA 34c gene in a biological sample collected from the subject. Also disclosed is a therapeutic agent for cancer, which comprises a nucleic acid encoding a BTG4 gene or BTG4, or comprises a nucleic acid encoding a miR-34b gene and/or an miR-34c gene or miR-34b and/or miR-34c.

摘要翻译： 该目的旨在全面分析在癌症中经历表观遗传学沉默的miRNA，以鉴定与癌症相关的miRNA，阐明鉴定的miRNA在癌症中的作用，并开发出一种检测癌症的新方法和一种新的癌症治疗剂， miRNA。 公开了一种用于检测受试者中癌症的方法，其包括检测从受试者收集的生物样品中位于微小RNA 34b基因和/或微小RNA 34c基因的启动子区域中的CpG岛中的甲基化CpG。 还公开了一种癌症治疗剂，其包含编码BTG4基因或BTG4的核酸，或包含编码miR-34b基因和/或miR-34c基因或miR-34b和/或miR-34c的核酸 。

公开(公告)号：EP2022861B1

公开(公告)日：2013-11-06

申请号：EP07743388.6

申请日：2007-05-15

申请人： Sapporo Medical University ,  St. Marianna University School of Medicine ,  Hitachi Chemical Company, Ltd.

发明人： WATANABE, Yoshiyuki ,  TOYOTA, Minoru ,  IMAI, Kohzo ,  SHINOMURA, Yasuhisa ,  ITOH, Fumio ,  TOKINO, Takashi

IPC分类号： C12Q1/68 ,  C12M1/00 ,  C12M1/26 ,  C12M3/00 ,  G01N33/15 ,  C12N15/09

CPC分类号： C12Q1/6806

公开(公告)号：EP2472257A1

公开(公告)日：2012-07-04

申请号：EP10812045.2

申请日：2010-08-30

申请人： Sapporo Medical University

发明人： TOYOTA, Minoru ,  YAMAMOTO, Eiichiro ,  KAMIMAE, Seiko ,  SUZUKI, Hiromu ,  YAMANO, Hiroo

IPC分类号： G01N33/50 ,  A61B1/00 ,  C12M3/00 ,  C12Q1/02 ,  C12Q1/68

CPC分类号： G01N33/57419 ,  A61B1/041 ,  C12Q1/6886 ,  C12Q2600/154 ,  C12Q2600/178 ,  G01N33/57488

摘要： Provided is a method for non-invasively obtaining an index for diagnosing the infiltration or invasive depth of a large intestine tumor. The method is characterized in that a specimen by which the infiltrative large intestine tumor can be detected is obtained by spraying a cleaning solvent onto the target large intestine mucous layer to separate the mucous from said mucous layer, and recovering the separated mucous along with the cleaning solvent.

摘要翻译： 本发明提供了非侵入性地获得用于诊断大肠肿瘤的浸润深度的指标的方法。 该方法的特征在于，可以通过将清洁溶剂喷射到目标大肠粘膜层上来分离粘液从粘液层中分离粘液，同时清洗分离的粘液，从而获得可以检测渗透性大肠肿瘤的样本 溶剂。