公开(公告)号：EP2167643A2

公开(公告)日：2010-03-31

申请号：EP08826211.8

申请日：2008-06-06

申请人： Senomyx, Inc.

发明人： MOYER, Bryan ,  ZLOTNIK, Albert ,  HEVEZI, Peter ,  SOTO, Hortensia ,  LU, Min ,  GAO, Na ,  SERVANT, Guy ,  BRUST, Paul ,  WILLIAMS, Mark ,  KALABAT, Dalia ,  WHITE, Evan, Carl ,  LAITA, Bianca ,  DAHAN, David ,  SAGANICH, Michael

IPC分类号： C12N5/00

CPC分类号： G01N33/566 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/158 ,  C40B30/04 ,  C40B40/10 ,  G01N33/5005 ,  G01N33/5044 ,  G01N33/6872 ,  G01N2333/726 ,  G01N2500/10

摘要： This invention relates to novel rationale and methods for identifying human and primate taste-specific genes, including genes involved in salty taste perception, especially human salty taste perception, but also genes involved in sweet, bitter, umami, and sour taste perception, and genes involved in other taste cell or taste receptor related activities such as digestive function and digestive related diseases, taste cell turnover, immunoregulation of the oral and digestive tract, and metabolic regulation such as in diabetes and obesity, the genes identified using these methods, and assays for identifying taste modulators (enhancers or blockers) and potential therapeutics using these genes. These compounds have potential application in modulating (enhancing or blocking) taste perception, especially salty taste perception and as potential therapeutics. In addition, this invention relates to novel methods for identifying taste-specific genes that can be used as markers for different taste cell types, including sweet, bitter, umami, sour, salty, and other taste cells in mammals as well as assays that measure the activity of the sweet, bitter, umami, or sour receptor in the presence of these genes to identify modulators of sweet, bitter, umami, and sour taste and to identify therapeutics especially for treating digestive or metabolic disorders, taste loss, and oral infections. Particularly, the genes identified herein and antibodies or oligos thereto can be used as markers to identify and/or purify specific taste cells e.g., from taste cell suspensions by use of FACS or magnetic bead cell selection or other known cell purification and isolation procedures.

摘要翻译： 用于鉴定人TRPML3调节剂，优选TRPML3增强剂的高通量哺乳动物和中等通量的基于卵母细胞的电生理学测定法公开了在测定法中调节TRPML3功能的化合物预期会影响人类的咸味。电生理学测定法，诸如双电极 电压钳技术，有助于鉴定特异性调节人TRPML3的化​​合物。该测定法提供了用于检测促进（增强）或抑制TRPML3功能并由此调节咸味的化合物的有力筛选。此外，这些化合物可用于调节钠 排泄，泌尿量和其他与钠水平有关的生物学功能

公开(公告)号：EP1937897A2

公开(公告)日：2008-07-02

申请号：EP06826403.5

申请日：2006-10-19

申请人： Senomyx, Inc.

发明人： SERVANT, Guy ,  WILLIAMS, Mark ,  PATRON, Andrew ,  KAMDAR, Poonit ,  CHEN, Qing ,  MOYER, Bryan ,  DAHAN, David ,  DITSCHUN, Tanya ,  RAY, Sumita ,  LIGANI, Amy

IPC分类号： E01C3/06

CPC分类号： E01C3/06 ,  G01N33/566

摘要： Robust cell based assays are provided that use cells that express wild-type or modified TRPM5 nucleic acid sequences in order to identify putative taste modulators, preferably sweet, bitter and umami taste modulators. The preferred assays use HEK-293 cells that express TRPM5, optionally at least one GPCR, preferably a taste specific GPCR, and a G protein that couples therewith. These assays detect TRPM5 modulators by use of membrane potential dyes that emit fluorescence on changes in TRPM5 activity based on changes in membrane potential and these changes in fluorescence are detectable using Fluorimetric Imaging Plate Readers (FLIPR).

公开(公告)号：EP1934606A2

公开(公告)日：2008-06-25

申请号：EP06816320.3

申请日：2006-10-04

申请人： Senomyx, Inc.

发明人： SERVANT, Guy ,  BRUST, Paul ,  MOYER, Bryan ,  LU, Min ,  ECHEVERRI, Fernando ,  DAHAN, David ,  ZOLLER, Mark ,  WILLIAMS, Mark ,  KIMMICH, Rachel ,  KAMDAR, Poonit ,  DITSCHUN, Tanya ,  PATRON, Andrew

IPC分类号： G01N33/53

CPC分类号： C07K14/705

摘要： Modified human TRPM8 nucleic acid sequences which are efficiently expressed in human cells and cell-based assays and test kits containing same are provided. These assays identify TRPM8 modulators using cells that express a modified human TRPM8 nucleic acid sequence according to the invention, wherein said sequence has been modified relative to a wild-type human TRPM8 nucleic acid sequence in order to optimize ion channel expression in desired cells. Assays using these modified TRPM8 sequences have been shown to identify compounds that modulate the human TRPM8 ion channel better or comparably to known coolants such as menthol and icilin.