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公开(公告)号：EP4273581A1
公开(公告)日：2023-11-08
申请号：EP22745001.2
申请日：2022-01-05
发明人： ZHANG, Xiaojia , LUO, Puwen , WU, Yifei
摘要： The present disclosure discloses methods and systems for laser distance measuring, focusing methods and systems, and devices for auto-focusing analysis. The method for laser distance measuring includes: emitting an incident ray α having an incident angle δ to a reflective surface of a laser reflecting mirror (31), the incident ray α being reflected by the laser reflecting mirror (31) to generate a first reflected ray β, and the first reflected ray β irradiating an object to be measured (1000); capturing a second reflected ray θ and generating a laser image on a laser imaging plane (3221), the second reflected ray θ being generated by the reflective surface of the laser reflecting mirror (31) reflecting a return ray γ generated after the first reflected ray β irradiates a surface of the object to be measured; and determining, based on the laser image, a measurement distance A according to a geometrical trigonometry. The height of a base of a triangle is equal to the measurement distance A. The base of the triangle is a connection line L of an emitting point of the incident ray α and a receiving point of the second reflected ray θand a vertex angle of the triangle is an intersection angle of the incident ray α and an extension line of the second reflected ray θ.
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公开(公告)号：EP3318865A1
公开(公告)日：2018-05-09
申请号：EP16817065.2
申请日：2016-05-20
发明人： LUO, Puwen , XIA, Haohan
IPC分类号： G01N21/64
CPC分类号： G01N21/6458 , G01N2021/6471 , G01N2021/6478 , G01N2201/061 , G01N2201/062 , G02B21/06 , G02B21/16 , G02B21/36
摘要： A fluorescent microscopic imaging method and apparatus, comprising: according to experiment requirements, illuminating at least one monochromatic fluorescence excitation light source (101) of the same colour from amongst a plurality of monochromatic fluorescence excitation light sources (101) as a target light source, the monochromatic fluorescence excitation light emitted by each monochromatic fluorescence excitation light source (101) being obliquely emitted onto a preset detection area of a sample plate to be detected; on the side of the sample plate to be detected furthest from the target light source, collecting the fluorescence of the particles in the preset detection area excited by the irradiation of the monochromatic fluorescence excitation light emitted by the target light source, and amplifying the preset detection area to a preset multiple; implementing light-filtering processing of the excited fluorescence of the particles in the preset detection area; and acquiring a fluorescent image of the preset detection area. A two-colour mirror is not required, and the fluorescence microscope apparatus therefore has a simple structure and low cost, preventing light energy loss. As the monochromatic fluorescence excitation light is obliquely emitted onto the sample plate to be detected, a small amount of monochromatic fluorescence excitation light is emitted into an objective lens after passing through the sample plate to be detected.
摘要翻译： 1。一种荧光显微镜成像方法及装置，其特征在于，包括：根据实验要求，从多个单色荧光激发光源（101）中照射至少一个相同颜色的单色荧光激发光源（101）作为目标光源， 每个单色荧光激发光源（101）发出的单色荧光激发光斜向发射到待检样板的预设检测区域上; 在距离目标光源最远的待检测样板侧，收集目标光源发射的单色荧光激发光照射所激发的预设检测区域内的颗粒荧光，并放大预设检测值 面积达到预设倍数; 对所述预设检测区域内的粒子激发荧光进行滤光处理; 并获取预设检测区域的荧光图像。 不需要双色镜，因此荧光显微镜装置结构简单，成本低，防止光能损失。 当单色荧光激发光倾斜地发射到待检样板上时，少量的单色荧光激发光穿过待检样板发射到物镜中。
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4.发明公开
公开(公告)号：EP4212853A1
公开(公告)日：2023-07-19
申请号：EP21885279.6
申请日：2021-10-28
发明人： LUO, Puwen , JIANG, Jing , CHEN, Kai , FAN, Weiya
摘要： The embodiments of the present disclosure provide a method, a system, and an application for detecting at least one of a cell-killing efficacy or an immune activity. The method comprises: obtaining a plurality of microscopic images of a fixed area of a co-culture sample, wherein the co-culture sample is a cell sample obtained by co-culturing target cells and effector cells, the fixed area of the co-culture sample includes a plurality of objects, wherein the plurality of objects are a cell group including cells with different properties, each of the plurality of objects having an image-identifiable feature; performing an image overlapping synthesis analysis or an image fusion analysis for the plurality of microscopic images to obtain the cell properties of the plurality of objects and make statistics to cell parameters associated with the cell properties; and evaluating at least one of the cell-killing efficacy or the immune activity of the effector cells based on the cell parameters.
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公开(公告)号：EP4063932A1
公开(公告)日：2022-09-28
申请号：EP20899104.2
申请日：2020-12-08
发明人： CHEN, Rui , LUO, Puwen , XIA, Haohan
摘要： The present disclosure discloses a microscopic device (100, 1400, 1900) and an image processing device (1500). The image processing device (1500) includes a receiving device (1501) configured to receive a digital image generated by the microscopic device (100, 1400, 1900), the digital image includes a scaling pattern (202, 302, 402, 502, 702, 802, 902, 1002); a storage device (1502) configured to store the digital image; and a processor (1503) configured to determine a magnification of the microscopic device (100, 1400, 1900) based on the scaling pattern (202, 302, 402, 502, 702, 802, 902, 1002).
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公开(公告)号：EP3657154A1
公开(公告)日：2020-05-27
申请号：EP18835191.0
申请日：2018-07-11
发明人： YAN, Haibo , HUANG, Haiqing , LUO, Puwen
IPC分类号： G01N15/14
摘要： A method and system for determining the fluorescence intensity of a fluorescence image. The method comprises: carrying out bright-field imaging and fluorescence imaging on a target sample by using a microscopic fluorescence imaging system to obtain a bright field image and a fluorescence image; carrying out edge extraction and segmentation on a fluorescence image region of each detected target in the fluorescence image to obtain a fluorescence image region corresponding to each detected target in the fluorescence image; and calculating and obtaining a cumulative gray-scale value, a maximum gray-scale value and an average gray-scale value of the fluorescence image region of each detected target, and a diameter value of a bright field image region of each detected target, and carrying out flow cluster analysis based on the cumulative gray-scale value, the maximum gray-scale value, the average gray-scale value and the bright field diameter value. The method can be developed or input to flow cluster analysis software for analysis to obtain results corresponding to and similar to flow analysis.
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公开(公告)号：EP4344777A3
公开(公告)日：2024-05-29
申请号：EP23195842.2
申请日：2023-09-06
发明人： LUO, Puwen
IPC分类号： B01L3/00
CPC分类号： B01L3/5027 , B01L2200/1620130101 , B01L2300/081620130101 , B01L2300/08720130101 , B01L2400/040620130101 , B01L2400/04920130101 , B01L2400/068820130101 , B01L2200/062120130101 , B01L2400/063320130101 , B01L2400/069420130101 , G01N2015/100620130101
摘要： The embodiments of the present disclosure provide a sample analysis device and a sample analysis system. The sample analysis device includes a base and one or more sample analysis units provided on the base. Each sample analysis unit includes a liquid storage chamber, an optical imaging chamber, a drainage chamber, and a sealing element. The liquid storage chamber is arranged on the base. The optical imaging chamber is arranged on the base, and a side of the optical imaging chamber is connected to the liquid storage chamber. The drainage chamber is arranged on the base. The drainage chamber is connected to the other side of the optical imaging chamber, and the drainage chamber is provided with an opening connecting with atmosphere. The sealing element removably seals the opening.
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公开(公告)号：EP4344777A2
公开(公告)日：2024-04-03
申请号：EP23195842.2
申请日：2023-09-06
发明人： LUO, Puwen
IPC分类号： B01L3/00
摘要： The embodiments of the present disclosure provide a sample analysis device and a sample analysis system. The sample analysis device includes a base and one or more sample analysis units provided on the base. Each sample analysis unit includes a liquid storage chamber, an optical imaging chamber, a drainage chamber, and a sealing element. The liquid storage chamber is arranged on the base. The optical imaging chamber is arranged on the base, and a side of the optical imaging chamber is connected to the liquid storage chamber. The drainage chamber is arranged on the base. The drainage chamber is connected to the other side of the optical imaging chamber, and the drainage chamber is provided with an opening connecting with atmosphere. The sealing element removably seals the opening.
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公开(公告)号：EP3133433A1
公开(公告)日：2017-02-22
申请号：EP15875206.3
申请日：2015-12-29
发明人： LUO, Puwen , CHEN, Rui
CPC分类号： G02B21/26 , G02B21/00 , G02B21/0008 , G02B21/241 , G02B21/362 , G02B25/005
摘要： A follow-up fixed focus system. The system comprises a fixed focus device (300), and the second end of the fixed focus device (300) is in contact with a sample plate (500); when the sample plate (500) has a small protrusion, the second end of the fixed focus device (300) is pushed by the protrusion of the sample plate (500). When the sample plate (500) has a small depression, the second end of the fixed focus device (300) is in contact with the sample plate (500) all the time under the action of self gravity of an observation device (100), so that the imaging distance between an object lens (103) and the sample plate (500) is kept unchanged. The system is realized by only adding one fixed focus device (300), and the fixed focus device (300) is simple in structure, low in manufacturing cost, easy to achieve, good in stability and capable of solving the problem that focus deviation of sample imaging is caused along with movement of a movable platform.
摘要翻译： 后续的固定焦点系统。 该系统包括固定聚焦装置（300），并且固定聚焦装置（300）的第二端与样品板（500）接触; 当样品板（500）具有小突起时，定焦装置（300）的第二端被样品板（500）的突起推动。 当样品板（500）具有小的凹陷时，定影聚焦装置（300）的第二端在观察装置（100）的自重的作用下总是与样品板（500）接触， 使得物镜（103）和样品板（500）之间的成像距离保持不变。 该系统仅增加一个定焦装置（300）即可实现，而定焦装置（300）结构简单，制造成本低，易于实现，稳定性好，能够解决焦点偏移 样品成像随着可移动平台的移动而引起。
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