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    METHOD FOR SPECIFIC CLEAVAGE OF C(ALPHA)-C BOND AND SIDE CHAIN OF PROTEIN AND PEPTIDE, AND METHOD FOR DETERMINING AMINO ACID SEQUENCE
    4.
    发明公开
    METHOD FOR SPECIFIC CLEAVAGE OF C(ALPHA)-C BOND AND SIDE CHAIN OF PROTEIN AND PEPTIDE, AND METHOD FOR DETERMINING AMINO ACID SEQUENCE 审中-公开
    特异性裂解蛋白质和肽的C（α）-C键和侧链的方法以及测定氨基酸序列的方法

    公开(公告)号：EP3246706A1

    公开(公告)日：2017-11-22

    申请号：EP17170513.0

    申请日：2017-05-10

    申请人： Shimadzu Corporation ,  National University of Corporation Hiroshima University

    发明人： IZUMI, Shunsuke ,  TANAKA, Koichi ,  FUKUYAMA, Yuko

    IPC分类号： G01N33/68 ,  G01N27/64 ,  H01J49/16

    CPC分类号： C07K1/128 ,  C07C205/59 ,  C07C205/60 ,  G01N33/6842 ,  G01N33/6851 ,  H01J49/0059

    摘要： The present invention provides a method for specifically cleaving a Cα-C bond of a peptide backbone and/or a side chain of a protein and a peptide, and a method for determining amino acid sequences of protein and peptide. A method for specifically cleaving a Cα-C bond of a peptide backbone and/or a side chain bond of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of at least one hydroxynitrobenzoic acid selected from the group consisting of 3-hydroxy-2-nitrobenzoic acid, 4-hydroxy-3-nitrobenzoic acid, 5-hydroxy-2-nitrobenzoic acid, 3-hydroxy-5-nitrobenzoic acid, and 4-hydroxy-2-nitrobenzoic acid. A method for determining an amino acid sequence of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of the above specific hydroxynitrobenzoic acid to specifically cleave a Cα-C bond of a peptide backbone and/or a side chain bond, and analyzing generated fragment ions by mass spectrometry.

    摘要翻译： 本发明提供了特异性切割肽骨架和/或蛋白质和肽的侧链的Cα-C键的方法，以及测定蛋白质和肽的氨基酸序列的方法。 一种用于特异性切割蛋白质或肽的肽主链和/或侧链键的Cα-C键的方法，其包括在至少一种选自以下的羟基硝基苯甲酸存在下用激光照射蛋白质或肽： 由3-羟基-2-硝基苯甲酸，4-羟基-3-硝基苯甲酸，5-羟基-2-硝基苯甲酸，3-羟基-5-硝基苯甲酸和4-羟基-2-硝基苯甲酸组成的组。 一种确定蛋白质或肽的氨基酸序列的方法，包括在上述特定的羟基硝基苯甲酸存在下用激光照射蛋白质或肽以特异性切割肽骨架和/或肽的Cα-C键 侧链键，并通过质谱分析产生的碎片离子。

    HYDROPHILIC THIOL PROBE
    5.
    发明公开
    HYDROPHILIC THIOL PROBE 审中-公开
    亲水硫醇探针

    公开(公告)号：EP2755024A1

    公开(公告)日：2014-07-16

    申请号：EP12830678.4

    申请日：2012-08-17

    申请人： Shimadzu Corporation

    发明人： SHIMADA, Takashi ,  SATO, Taka-Aki ,  TANAKA, Koichi

    IPC分类号： G01N27/62

    CPC分类号： G01N33/6848 ,  Y10T436/145555 ,  Y10T436/147777 ,  Y10T436/173845 ,  Y10T436/19 ,  Y10T436/200833 ,  Y10T436/24

    摘要： The present invention provides a probe that further promotes ionization in proteomic analysis using mass spectrometry, and a high-sensitive mass spectrometry method for a protein using such a probe. Further, the present invention provides an ionization-enhancing probe that can be used even for a protein that has a high degree of hydrophobicity and quickly turns over, and a high-sensitive mass spectrometry method for a protein using such a probe.
    A thiol probe for a protein, which is represented by the following formula (I): [Chemical Formula 1]

    wherein R 1 represents a linker group, and R 2 represents a substituted ammonium group or a substituted amino group. A mass spectrometry method for a protein, comprising the steps of: obtaining a modified protein by reacting the thiol probe with a protein to be subjected to mass spectrometry; and subjecting the modified protein to mass spectrometry.

    摘要翻译： 本发明提供了使用质谱法进一步促进蛋白质组分析中的离子化的探针，以及使用这种探针的蛋白质的高灵敏度质谱法。 此外，本发明提供了即使对于疏水性高且快速翻转的蛋白质也可以使用的电离增强探针，以及使用这种探针的蛋白质的高灵敏度质谱分析方法。 用于蛋白质的硫醇探针，其由下式（I）表示：[化学式1]其中R 1表示连接基团，且R 2表示取代的铵基或取代的氨基。 一种蛋白质的质量分析方法，包括以下步骤：通过使巯基探针与待进行质谱分析的蛋白质反应获得修饰的蛋白质; 并将修饰的蛋白质进行质谱分析。