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    METHOD FOR PREPARING PEPTIDE FRAGMENTS, KIT FOR PREPARING PEPTIDE FRAGMENTS TO BE USED THEREIN, AND ANALYSIS METHOD
    2.
    发明公开
    METHOD FOR PREPARING PEPTIDE FRAGMENTS, KIT FOR PREPARING PEPTIDE FRAGMENTS TO BE USED THEREIN, AND ANALYSIS METHOD 有权
    制备肽片段的方法，制备用于其中的肽片段的试剂盒以及分析方法

    公开(公告)号：EP3023777A1

    公开(公告)日：2016-05-25

    申请号：EP13893016.9

    申请日：2013-09-09

    申请人： Shimadzu Corporation

    发明人： SHIMADA, Takashi ,  FUKAO, Noriko ,  AOKI, Chikage ,  SATO, Taka-Aki

    IPC分类号： G01N27/62 ,  G01N1/28

    CPC分类号： G01N33/6857 ,  C07K1/107 ,  C07K1/12 ,  G01N1/405 ,  G01N33/6851 ,  G01N2333/976 ,  G01N2560/00

    摘要： A method for preparing peptide fragments by cleaving a protein using a protease. The method according to the present invention comprises a step for contacting, in a liquid, a porous body (20), in which the substrate protein (25) to be cleaved is immobilized in pores (29), with microparticles (10) which carry the protease (15) immobilized on the surface thereof. In the present invention, the average particle diameter of the microparticles (10) is larger than the average pore diameter of the porous body (20). According to the method of the present invention, the substrate protein (25) that is an antibody can be site-specifically cleaved. By analyzing the peptide fragments that are obtained by the above method by mass spectrometry, etc., the antibody protein can be detected or quantitatively analyzed.

    摘要翻译： 用蛋白酶切割蛋白质制备肽片段的方法。 根据本发明的方法包括以下步骤：在液体中使待分离的底物蛋白（25）固定在孔（29）中的多孔体（20）与携带微粒（10）的微粒 固定在其表面上的蛋白酶（15）。 在本发明中，微粒（10）的平均粒径大于多孔体（20）的平均细孔径。 根据本发明的方法，可以对作为抗体的底物蛋白质（25）进行位点特异性切割。 通过分析通过上述方法通过质谱法等获得的肽片段，可以检测或定量分析抗体蛋白质。

    HYDROPHILIC THIOL PROBE
    3.
    发明公开
    HYDROPHILIC THIOL PROBE 审中-公开
    亲水硫醇探针

    公开(公告)号：EP2755024A1

    公开(公告)日：2014-07-16

    申请号：EP12830678.4

    申请日：2012-08-17

    申请人： Shimadzu Corporation

    发明人： SHIMADA, Takashi ,  SATO, Taka-Aki ,  TANAKA, Koichi

    IPC分类号： G01N27/62

    CPC分类号： G01N33/6848 ,  Y10T436/145555 ,  Y10T436/147777 ,  Y10T436/173845 ,  Y10T436/19 ,  Y10T436/200833 ,  Y10T436/24

    摘要： The present invention provides a probe that further promotes ionization in proteomic analysis using mass spectrometry, and a high-sensitive mass spectrometry method for a protein using such a probe. Further, the present invention provides an ionization-enhancing probe that can be used even for a protein that has a high degree of hydrophobicity and quickly turns over, and a high-sensitive mass spectrometry method for a protein using such a probe.
    A thiol probe for a protein, which is represented by the following formula (I): [Chemical Formula 1]

    wherein R 1 represents a linker group, and R 2 represents a substituted ammonium group or a substituted amino group. A mass spectrometry method for a protein, comprising the steps of: obtaining a modified protein by reacting the thiol probe with a protein to be subjected to mass spectrometry; and subjecting the modified protein to mass spectrometry.

    摘要翻译： 本发明提供了使用质谱法进一步促进蛋白质组分析中的离子化的探针，以及使用这种探针的蛋白质的高灵敏度质谱法。 此外，本发明提供了即使对于疏水性高且快速翻转的蛋白质也可以使用的电离增强探针，以及使用这种探针的蛋白质的高灵敏度质谱分析方法。 用于蛋白质的硫醇探针，其由下式（I）表示：[化学式1]其中R 1表示连接基团，且R 2表示取代的铵基或取代的氨基。 一种蛋白质的质量分析方法，包括以下步骤：通过使巯基探针与待进行质谱分析的蛋白质反应获得修饰的蛋白质; 并将修饰的蛋白质进行质谱分析。

    MASS SPECTROMETRY OF BIOLOGICAL SAMPLE USING IMMUNOPRECIPITATION
    4.
    发明公开
    MASS SPECTROMETRY OF BIOLOGICAL SAMPLE USING IMMUNOPRECIPITATION 有权
    马萨诸塞州生物学家探索者

    公开(公告)号：EP2088426A1

    公开(公告)日：2009-08-12

    申请号：EP07829381.8

    申请日：2007-10-02

    申请人： Shimadzu Corporation

    发明人： SHIMADA, Takashi ,  TOYAMA, Atsuhiko ,  SATO, Taka-Aki

    IPC分类号： G01N27/62 ,  G01N33/48 ,  G01N33/483

    CPC分类号： G01N33/6848

    摘要： It is intended to provide an analysis method for a biological sample with clinical significance, which is a system being simple to such an extent that it can be applied to a clinical field, and capable of capping a molecule ionization suppression in mass spectrometry. Mass spectrometry of a biological sample using immunoprecipitation, comprising the steps of: preparing a sample containing an objective biological molecule; concentrating the biological molecule in the sample by immunoprecipitation using an antibody-bound carrier or a carrier to which a molecule capable of specifically binding to an antibody is bound; washing an immunoprecipitate of the biological molecule by using an aqueous solution containing a charge neutralizing agent as a Mashing liquid; and detecting the biological molecule by subjecting the washed immunoprecipitate to mass spectrometry.

    摘要翻译： 旨在为具有临床意义的生物样品提供分析方法，该方法简单到能够应用于临床领域并且能够在质谱中封端分子离子化抑制的系统。 使用免疫沉淀的生物样品的质谱法，包括以下步骤：制备含有客观生物分子的样品; 通过使用抗体结合的载体或能够与抗体特异性结合的分子结合的载体通过免疫沉淀将生物分子浓缩在样品中; 通过使用含有电荷中和剂的水溶液作为糊化液来洗涤生物分子的免疫沉淀物; 并通过对经洗涤的免疫沉淀物进行质谱检测来检测生物分子。

    METHOD FOR DETECTING GRANULAR SUBSTANCE BY IMMUNOCHROMATOGRAPHY METHOD, AND KIT FOR SAME
    7.
    发明公开
    METHOD FOR DETECTING GRANULAR SUBSTANCE BY IMMUNOCHROMATOGRAPHY METHOD, AND KIT FOR SAME 审中-公开

    公开(公告)号：EP4257979A1

    公开(公告)日：2023-10-11

    申请号：EP21903387.5

    申请日：2021-12-07

    申请人： Dai Nippon Toryo Co., Ltd. ,  Shimadzu Corporation

    发明人： MIYAZAWA ,Yuta ,  FUJIMOTO, Hirotaka ,  WATANABE, Makoto ,  SATO, Taka-Aki

    IPC分类号： G01N33/543

    摘要： An object of the present invention is to provide a method for detecting particulate substances with a high degree of sensitivity, while suppressing the generation of non-specific signals.
    According to the present invention, the generation of non-specific signals in the background can be suppressed by blocking a membrane of a test strip which is used for immunochromatography with a blocking composition comprising a polymer-based blocking agent having, in its side chain, a substituent represented by the following formula:

    (wherein R 1 , R 2 , and R 3 are each independently a hydrogen atom, a C 1-6 alkyl group, or a C 1-6 hydroxyalkyl group; and n is an integer of 1 to 4), and having a weight average molecular weight (Mw) of 1 × 10 3 to 1 × 10 7 .

    METHOD FOR IDENTIFYING PROTEINS
    9.
    发明公开
    METHOD FOR IDENTIFYING PROTEINS 审中-公开

    公开(公告)号：EP3739328A1

    公开(公告)日：2020-11-18

    申请号：EP18899287.9

    申请日：2018-01-09

    申请人： Shimadzu Corporation

    发明人： SONOMURA, Kazuhiro ,  SATO, Taka-Aki ,  MATSUDA, Fumihiko ,  WANG Yi-Ting ,  ISHIHAMA, Yasushi ,  TSAI Chia-Feng

    IPC分类号： G01N27/62

    摘要： Provided is a method for identifying proteins capable of increasing the number of identified proteins contained in a target sample derived from blood. A protein contained in the target sample derived from blood is fragmented, and a protein contained in an having less bias in a quantity ratio of proteins than the target sample is fragmented, and the fragmented proteins are mixed (Steps S101 to S103). In this manner, the mixed sample in which the bias in a quantity ratio of proteins is less than that of the target sample is generated. By performing MS/MS measurement using the generated mixed sample (Step S107), an MS/MS spectrum of a peak derived from a protein contained in a small amount in the target sample can be prevented from being missed. Accordingly, the number of identified proteins contained in the target sample derived from blood can be increased.