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6 条记录 (耗时 0.012 秒)

    REDUCTION OF NON-SPECIFIC BINDING IN ASSAYS
    1.
    发明公开
    REDUCTION OF NON-SPECIFIC BINDING IN ASSAYS 有权
    减少测定中的非特异性结合

    公开(公告)号：EP1960783A1

    公开(公告)日：2008-08-27

    申请号：EP05826152.0

    申请日：2005-11-17

    申请人： Siemens Healthcare Diagnostics Inc.

    发明人： TENG, Zhu ,  MOORE, Jeffrey. L. ,  CRAIG, Alan, R. ,  HICKEY, Gary ,  SCHELP, Carsten ,  WEI, Tie, Quan

    IPC分类号： G01N33/544 ,  G01N33/548 ,  G01N33/549 ,  G01N33/53 ,  C07K1/10

    CPC分类号： C07K9/00 ,  A61K38/00 ,  G01N33/54393 ,  G01N33/548 ,  G01N33/549

    摘要： Methods and compositions are disclosed for reducing non-specific binding in a binding assay for the determination of an analyte in a sample where one of the reagents for conducting the binding assay comprises a solid support comprising a polysaccharide. The method comprises including in an assay medium for conducting the binding assay a soluble compound comprising a protein linked to a polysaccharide. Also disclosed are methods and compositions for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The methods include as reagents a solid support comprising a polysaccharide and a soluble compound comprising a protein linked to a polysaccharide.

    摘要翻译： 公开了用于减少用于确定样品中分析物的结合测定中的非特异性结合的方法和组合物，其中用于进行结合测定的一种试剂包含含有多糖的固体支持物。 该方法包括在用于进行结合测定的测定培养基中包含包含与多糖连接的蛋白质的可溶性化合物。 还公开了用于确定怀疑含有分析物的样品中分析物的存在和/或量的方法和组合物。 该方法包括作为试剂的包含多糖和包含与多糖连接的蛋白质的可溶性化合物的固体支持物。

    IMMUNOASSAYS EMPLOYING NON-PARTICULATE CHEMILUMINESCENT REAGENT
    2.
    发明授权
    IMMUNOASSAYS EMPLOYING NON-PARTICULATE CHEMILUMINESCENT REAGENT 有权
    使用非粒子化学发光试剂的免疫分析

    公开(公告)号：EP2406630B1

    公开(公告)日：2018-01-17

    申请号：EP10751173.5

    申请日：2010-02-25

    申请人： Siemens Healthcare Diagnostics Inc.

    发明人： SINGH, Pratap ,  ZHENG, Yi, Feng ,  GENG, Liping ,  JANZEN, Roland ,  SCHELP, Carsten

    IPC分类号： G01N33/50 ,  G01N33/58

    CPC分类号： G01N33/78 ,  G01N33/582 ,  G01N2333/59

    摘要： Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with chemiluminescent reagents for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The reagent is non-particulate and comprises a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. In embodiments of an assay, a combination is provided that comprises a sample suspected of containing the analyte, a chemiluminescent reagent as described above and a sensitizer reagent capable of generating singlet oxygen. The combination is subjected to conditions for binding of the analyte to the binding partner for the analyte. The sensitizer is activated and the amount of luminescence generated by the chemiluminescent composition is detected wherein the amount of luminescence is related to the amount of the analyte in the sample.

    STABILIZATION OF SIGNAL GENERATION IN PARTICLES USED IN ASSAYS
    5.
    发明公开
    STABILIZATION OF SIGNAL GENERATION IN PARTICLES USED IN ASSAYS 审中-公开
    信号的用于生产的试验中使用粒子镇定

    公开(公告)号：EP2491093A1

    公开(公告)日：2012-08-29

    申请号：EP10825530.8

    申请日：2010-10-19

    申请人： Siemens Healthcare Diagnostics Inc.

    发明人： CRAIG, Alan, R. ,  TENG, Zhu ,  SCHELP, Carsten ,  SNYDER, Jason ,  MORAN, Christine

    IPC分类号： C09K11/06

    CPC分类号： G01N21/76 ,  G01N21/6428 ,  G01N33/54393 ,  Y10S435/968 ,  Y10T436/10 ,  Y10T436/2525

    摘要： Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with a solid support such as, for example, a particle. The support comprises a chemiluminescent composition that comprises a metal chelate. The present inventors observed that, when such support such as, e.g., particles, were employed in assays for the determination of an analyte, stability of signal output by the chemiluminescent composition associated with the particle was unacceptably reduced as compared to particles comprising other chemiluminescent compositions. In accordance with embodiments of the present invention, the stability of signal output from such particles is enhanced by including in a medium comprising the particles a sufficient amount of one or more stabilizing agents, which may be a chelating agent and/or a metal chelate such as, for example, the metal chelate that is associated with the particle.

    IMMUNOASSAYS EMPLOYING NON-PARTICULATE CHEMILUMINESCENT REAGENT
    6.
    发明公开
    IMMUNOASSAYS EMPLOYING NON-PARTICULATE CHEMILUMINESCENT REAGENT 有权
    使用非粒子化学发光试剂的免疫分析

    公开(公告)号：EP2406630A1

    公开(公告)日：2012-01-18

    申请号：EP10751173.5

    申请日：2010-02-25

    申请人： Siemens Healthcare Diagnostics Inc.

    发明人： SINGH, Pratap ,  ZHENG, Yi, Feng ,  GENG, Liping ,  JANZEN, Roland ,  SCHELP, Carsten

    IPC分类号： G01N33/50

    CPC分类号： G01N33/78 ,  G01N33/582 ,  G01N2333/59

    摘要： Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with chemiluminescent reagents for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The reagent is non-particulate and comprises a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. In embodiments of an assay, a combination is provided that comprises a sample suspected of containing the analyte, a chemiluminescent reagent as described above and a sensitizer reagent capable of generating singlet oxygen. The combination is subjected to conditions for binding of the analyte to the binding partner for the analyte. The sensitizer is activated and the amount of luminescence generated by the chemiluminescent composition is detected wherein the amount of luminescence is related to the amount of the analyte in the sample.

    摘要翻译： 公开了用于进行测定的方法和试剂。 本发明方法和试剂的实施方式涉及用于确定怀疑含有分析物的样品中分析物的存在和/或量的化学发光试剂。 该试剂是非颗粒的并且包含分析物的结合配偶体和包含烯烃化合物和金属螯合物的化学发光组合物。 在测定的实施方案中，提供包含怀疑含有分析物的样品，如上所述的化学发光试剂和能够产生单线态氧的敏化剂的组合。 使组合受到分析物与分析物结合配偶体结合的条件。 敏化剂被激活并且检测由化学发光组合物产生的发光量，其中发光量与样品中分析物的量相关。