公开(公告)号：EP4159862A1

公开(公告)日：2023-04-05

申请号：EP21812733.0

申请日：2021-05-25

申请人： Spiber Inc.

发明人： LIPS David ,  VERKLEIJ Gijs

IPC分类号： C12N15/62 ,  C12N5/10 ,  C12N15/76 ,  C40B40/06

摘要： The present invention relates to a method of preparing a gene cluster construct including a plurality of genes encoding a multi-modular biosynthetic enzyme, the method including (A) a step of preparing a plurality of DNA fragments which are capable of reconstructing the gene cluster construct and have structures that can be ligated to each other and (B) a step of ligating the plurality of DNA fragments prepared in the step (A) to each other by mixing the plurality of DNA fragments in a solution to obtain the gene cluster construct.

公开(公告)号：EP4159861A1

公开(公告)日：2023-04-05

申请号：EP21814439.2

申请日：2021-05-25

申请人： Spiber Inc.

发明人： LIPS David ,  VERKLEIJ Gijs

IPC分类号： C12N15/52 ,  C12N15/63 ,  C12N9/00

摘要： The present invention relates to a method for preparing a plasmid containing DNA that encodes a Type I polyketide synthase (PKS), the method including a step of introducing a DNA construct containing tandem repeats of DNA that encodes a PKS into a Bacillus subtilis competent cell.