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    FUSION PROTEINS, RECOMBINANT BACTERIA, AND METHODS FOR USING RECOMBINANT BACTERIA
    5.
    发明公开
    FUSION PROTEINS, RECOMBINANT BACTERIA, AND METHODS FOR USING RECOMBINANT BACTERIA 审中-公开
    融合蛋白，重组细菌和使用重组细菌的方法

    公开(公告)号：EP3194425A1

    公开(公告)日：2017-07-26

    申请号：EP15772154.9

    申请日：2015-09-17

    申请人： Spogen Biotech Inc.

    发明人： THOMPSON, Brian ,  SIEGEL, Ashley

    IPC分类号： C07K14/32

    CPC分类号： C07K14/32 ,  A01N37/44 ,  A01N63/02 ,  A23K10/18 ,  A23K20/147 ,  A61K39/00 ,  A61L2/18 ,  C02F3/342 ,  C02F3/348 ,  C02F2101/10 ,  C02F2101/30 ,  C02F2103/002 ,  C02F2103/003 ,  C02F2103/007 ,  C02F2103/06 ,  C02F2103/10 ,  C02F2103/14 ,  C02F2103/16 ,  C02F2103/26 ,  C02F2103/28 ,  C07K2319/01 ,  C07K2319/035 ,  C07K2319/40 ,  C09K8/62 ,  C09K2208/24 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  E21B43/16

    摘要： Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

    摘要翻译： 提供了融合蛋白，其含有靶向序列，外孢壁蛋白或将融合蛋白靶向蜡状芽孢杆菌家族成员的外孢壁的外孢壁蛋白片段。 还提供了表达这种融合蛋白的重组蜡状芽孢杆菌家族成员。 还提供了基因失活的蜡状芽孢杆菌家族成员和过表达外孢菌蛋白的重组蜡状芽孢杆菌家族成员。 还提供了用重组蜡状芽孢杆菌家族成员包被的种子和使用重组蜡状芽孢杆菌家族成员（例如，用于刺激植物生长）的方法。 进一步提供了表达融合蛋白的重组蜡样芽孢杆菌家族成员的各种修饰。 还提供了包含孢子外壳蛋白和感兴趣的蛋白质或肽的融合蛋白，表达此类融合蛋白的重组细菌，用此类重组细菌包被的种子，以及使用这种重组细菌的方法（例如用于刺激植物生长）。

    COMPOSITIONS COMPRISING RECOMBINANT BACILLUS CELLS AND ANOTHER BIOLOGICAL CONTROL AGENT
    6.
    发明公开
    COMPOSITIONS COMPRISING RECOMBINANT BACILLUS CELLS AND ANOTHER BIOLOGICAL CONTROL AGENT 审中-公开
    包含重组BACILLUS细胞和另一种生物控制剂的组合物

    公开(公告)号：EP3193619A1

    公开(公告)日：2017-07-26

    申请号：EP15772140.8

    申请日：2015-09-17

    申请人： Bayer CropScience LP ,  Spogen Biotech Inc.

    发明人： CURTIS, Damian ,  THOMPSON, Brian

    IPC分类号： A01N63/00 ,  A01N63/04 ,  A01P21/00

    CPC分类号： C07K14/325 ,  A01N25/00 ,  A01N63/00 ,  A61K38/00 ,  C12N15/00 ,  C12N15/63 ,  C12N15/82 ,  C12N15/866 ,  A01N63/04

    摘要： The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one further biological control agent selected from particular microorganisms disclosed herein and/or a mutant of a specific strain of a microorganism disclosed herein having all identifying characteristics of the respective strain, and/or at least one metabolite produced by the respective strain, and/or at least one metabolite produced by the respective strain that exhibits activity against insects, mites, nematodes and/or phytopathogens in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and/or reducing overall damage of plants and plant parts.

    摘要翻译： 本发明涉及一种组合物，其包含：a）表达融合蛋白的产生重组芽孢杆菌细胞，所述融合蛋白包含：（i）至少一种植物生长刺激蛋白或肽和（ii）将融合蛋白定位于外生孢子 的芽孢杆菌细胞; 和b）选自本文公开的特定微生物的至少一种其他生物防治剂和/或本文公开的微生物特定菌株的突变体，其具有各自菌株的全部鉴定特征，和/或由各自产生的至少一种代谢产物 和/或由相应菌株产生的至少一种代谢物，其以协同有效量表现出对昆虫，螨，线虫和/或植物病原体的活性。 此外，本发明涉及该组合物的用途以及增强植物生长，促进植物健康，和/或减少植物和植物部分的总体损害的方法。

    FUSION PROTEINS AND METHODS FOR STIMULATING PLANT GROWTH, PROTECTING PLANTS, AND IMMOBILIZING BACILLUS SPORES ON PLANTS
    7.
    发明公开
    FUSION PROTEINS AND METHODS FOR STIMULATING PLANT GROWTH, PROTECTING PLANTS, AND IMMOBILIZING BACILLUS SPORES ON PLANTS 审中-公开
    融合蛋白的方法，以刺激植物生长，植物对植物的保护和固定BAZILLENSPOREN的

    公开(公告)号：EP2970507A1

    公开(公告)日：2016-01-20

    申请号：EP14763028.9

    申请日：2014-03-17

    申请人： Spogen Biotech Inc.

    发明人： THOMPSON, Brian ,  THOMPSON, Katie

    IPC分类号： C07K19/00 ,  C12N15/62 ,  C12N1/21

    CPC分类号： C07K14/32 ,  A01N37/46 ,  A01N37/48 ,  A01N63/00 ,  A01N63/02 ,  C07K2319/035 ,  C12N9/0095 ,  C12N9/16 ,  C12N9/20 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/48 ,  C12Y118/06001 ,  C12Y301/01003 ,  C12Y301/03008 ,  C12Y302/01004 ,  C12Y304/00

    摘要： The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins, formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth and for protecting plants from pathogens by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plant roots.

    摘要翻译： 本发明涉及含有一个靶向序列也目标融合蛋白的蜡状芽孢杆菌家族成员的芽孢外壁基因反弹融合蛋白。 因此，本发明涉及表达求融合蛋白，式灰含有表达融合蛋白质的重组蜡状芽孢杆菌家族成员重组蜡状芽孢杆菌的家庭成员。 因此描述了用于刺激植物生长和用于通过应用重组蜡状芽孢杆菌的家庭成员或式和灰到植物或植物生长介质保护从病原体的植物的方法。 因此，本发明涉及用于固定化表达对植物的根的融合蛋白的重组蜡状芽孢杆菌家族成员的孢子的方法。