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公开(公告)号：EP2245160A1
公开(公告)日：2010-11-03
申请号：EP09703905.1
申请日：2009-01-21
CPC分类号： C12N15/81 , C07K14/33 , C07K2319/02 , C07K2319/74 , C12N15/62 , C12N15/625
摘要： A yeast cell surface display system is described comprising a scaffoldin protein from Clostridium which includes at least one cohesin domain capable of binding to a dockerin domain, the scaffoldin protein displayable on the surface of the yeast cell, such as S. cerevisiae, and an anchoring protein capable of tethering the scaffoldin protein the yeast cell surface, such as cell wall protein 1 (Cwp 1 ). Cwp 1 is fused to the C-terminus of the scaffoldin protein and a secretion signal, such as Trichoderma reesei XYN2 is fused to the N-terminus of the chimeric scaffoldin protein. The scaffoldin protein is chimeric and comprises at least one C. cellulolyticum cohesin domain and at least one C. Thermocellum cohesin domain. The invention also includes a vector for expression of such a fusion protein.
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2.发明公开
公开(公告)号：EP3201335A1
公开(公告)日：2017-08-09
申请号：EP15779025.4
申请日：2015-10-02
IPC分类号： C12N9/26
CPC分类号： C12N9/2431 , A23L27/33 , A23L33/125 , A23V2002/00 , C12P19/16 , C12Y302/01026
摘要： The invention provides variants of the Aspergillus japonicus β-fructofuranosidase enzyme which have been modified so as to improve synthesis of inulin-type fructooligosaccharides (FOS) from sucrose. One or more substitutions may be made to the parent β-fructofuranosidase polypeptide at amino acid positions 121, 159, 302 and/or 471 of the mature peptide (SEQ ID NO: 3), corresponding to crystal positions 140, 178, 321 and 490. A method of synthesizing FOS using the variants is also claimed.
摘要翻译： 本发明提供了已被修饰以改善由蔗糖合成菊糖型低聚果糖（FOS）的日本曲霉β-呋喃果糖苷酶的变体。 可以在成熟肽（SEQ ID NO：3）的氨基酸位置（121,159,302和/或471）处对亲本β-呋喃果糖苷酶多肽进行一个或多个取代，其对应于结晶位置（140,178， 321和490）。 要求保护使用这些变体合成FOS的方法。
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公开(公告)号：EP3201335B1
公开(公告)日：2019-09-04
申请号：EP15779025.4
申请日：2015-10-02
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