公开(公告)号：EP4414028A2

公开(公告)日：2024-08-14

申请号：EP24171629.9

申请日：2016-05-19

申请人： Yale University

发明人： BRADDOCK, Demetrios ,  ALBRIGHT, Ronald

IPC分类号： A61P13/12

CPC分类号： A01K67/0271 ,  A01K2207/1520130101 ,  A01K2207/2020130101 ,  A01K2227/10520130101 ,  A01K2267/038720130101 ,  C07K14/745 ,  C07K2319/3020130101 ,  C07K2319/3120130101 ,  A61P9/10 ,  A61P13/12 ,  A61K38/46 ,  A61K38/465 ,  C12Y301/04001 ,  C12Y306/01009 ,  A61K38/04 ,  A61K38/1741 ,  C12N9/96 ,  C12N15/625 ,  A61K38/02

摘要： The present invention includes compositions and methods for treating diseases or disorders associated with pathological calcification or pathological ossification. In certain embodiments, the diseases or disorders are selected from the group consisting of Generalized Arterial Calcification of Infancy (GACI), Idiopathic Infantile Arterial Calcification (IIAC), Ossification of the Posterior Longitudinal Ligament (OPLL), hypophosphatemic rickets, osteoarthritis, calcification of atherosclerotic plaques, PXE, hereditary and non-hereditary forms of osteoarthritis, ankylosing spondylitis, hardening of the arteries occurring with aging, calciphylaxis resulting from end stage renal disease and progeria.

公开(公告)号：EP3000823A9

公开(公告)日：2018-11-14

申请号：EP14791458.4

申请日：2014-04-25

申请人： Nanjing University of Technology

发明人： HE, Bingfang ,  CHEN, Wenhua ,  MI, Lan ,  WU, Shanshan ,  ZHU, Yun ,  CHEN, Shanshan

IPC分类号： C07K5/103 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C12N15/70 ,  C12N1/21 ,  C12N9/24 ,  C12N9/42 ,  C12N15/11

CPC分类号： C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07K2319/02 ,  C12N9/2402 ,  C12N9/2454 ,  C12N15/625 ,  C12P21/02 ,  C12Y302/01 ,  C12Y302/01011

摘要： The present invention belongs to protein engineering and genetic engineering fields, relating to a strong secretory signal peptide enhancing small peptide motifs and the use thereof. The strong secretory signal peptide enhancing small peptide motifs of the present invention have the amino acid sequence of the following formula: M (αXβYγ/αYβXγ) n , wherein X represents an acidic amino acid; Y represents an alkaline amino acid; α is 0 to 2 neutral amino acid(s); β represents 0 to 2 neutral amino acid(s); γ represents 1 to 10 neutral amino acid(s); n is 1 to 3. With regard to the use of the strong secretory signal peptide enhancing small peptide motifs of the present invention, it is a method for constructing a vector enhancing the secretion ability of common signal peptides to improve the secretory expression of exogenous proteins.

公开(公告)号：EP3359671A2

公开(公告)日：2018-08-15

申请号：EP16854354.4

申请日：2016-10-06

申请人： DNA2.0, INC.

发明人： MINSHULL, Jeremy ,  WELCH, Mark ,  GOVINDRAJAN, Sridhar ,  LEE, Maggie ,  CAVES, Kate ,  NESS, Jon

IPC分类号： C12N15/74 ,  C12N15/67 ,  C12N15/11 ,  C12N15/85 ,  C12N15/87 ,  C12N15/90 ,  C12N15/867 ,  C12N1/19 ,  C12Q1/68 ,  A61K31/713

CPC分类号： C12N15/67 ,  C07K2319/09 ,  C12N9/12 ,  C12N9/1241 ,  C12N15/1082 ,  C12N15/52 ,  C12N15/625 ,  C12N15/63 ,  C12N15/81 ,  C12N15/815 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/90 ,  C12N2800/90 ,  C12N2830/007 ,  C12N2830/40 ,  C12N2830/42 ,  C12N2840/203 ,  C12R1/84 ,  C12R1/865 ,  C12Y207/00 ,  C12Y207/07

摘要： The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.

公开(公告)号：EP3344774A1

公开(公告)日：2018-07-11

申请号：EP16771006.0

申请日：2016-09-05

申请人： Keygene N.V.

发明人： VAN DIJK, Peter Johannes ,  RIGOLA, Diana ,  PRINS, Marinus Willem ,  VAN TUNEN, Adrianus Johannes

IPC分类号： C12N15/82 ,  C12Q1/68

CPC分类号： C12N15/8201 ,  C12N15/113 ,  C12N15/625 ,  C12N15/8202 ,  C12N15/8223 ,  C12N15/8287 ,  C12Q1/68 ,  C12Q1/6895 ,  C12Q2525/207 ,  C12Q2600/13

摘要： The invention provides nucleotide sequences and amino acid sequences of the Dipgene as well as (functional) homologues, fragments and variants thereof, which provides diplospory as a part of apomixis. Also diplospory plants and methods for making these are provided, as are methods of using these, and methods of making apomictic seed.

公开(公告)号：EP3199636A4

公开(公告)日：2018-04-11

申请号：EP15843689

申请日：2015-09-01

申请人： UNIV SANTIAGO COMPOSTELA

发明人： JOSE MANUEL MARTINEZ COSTAS ,  NATALIA BARREIRO PINEIRO ,  FRANCISCO JAVIER BENAVENTE MARTINEZ

IPC分类号： C12N15/46 ,  C07K14/14 ,  C07K19/00 ,  C12N5/10 ,  C12N15/62

CPC分类号： C12N15/625 ,  A61K39/00 ,  C07K14/005 ,  C07K14/14 ,  C07K19/00 ,  C07K2319/04 ,  C12N5/10 ,  C12N7/00 ,  C12N15/62 ,  C12N2720/12222 ,  C12N2760/20234 ,  G01N33/5076

摘要： The invention relates to a polynucleotide encoding a polypeptide based on the minimum region of the Orthoreovirus muNS protein that can form inclusions in the endoplasmic reticulum, and to said polypeptide. The invention also relates to a purification method and a method for detecting interaction between two polypeptides based on the capacity of some regions of the Orthoreovirus muNS protein to incorporate themselves into the inclusions, together with a peptide of interest.

公开(公告)号：EP3301106A1

公开(公告)日：2018-04-04

申请号：EP17200981.3

申请日：2014-04-25

申请人： Nanjing University of Technology

发明人： HE, Bingfang ,  CHEN, Wenhua ,  MI, Lan ,  WU, Shanshan ,  ZHU, Yun ,  CHEN, Shanshan

IPC分类号： C07K5/103 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C12N15/70 ,  C12N1/21 ,  C12N9/24 ,  C12N9/42 ,  C12N15/11

CPC分类号： C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07K2319/02 ,  C12N9/2402 ,  C12N9/2454 ,  C12N15/625 ,  C12P21/02 ,  C12Y302/01 ,  C12Y302/01011

摘要： The present invention belongs to protein engineering and genetic engineering fields, relating to a strong secretory signal peptide enhancing small peptide motifs and the use thereof. The strong secretory signal peptide enhancing small peptide motifs of the present invention have the amino acid sequence of the following formula: M (αXβYγ/αYβXγ) n , wherein X represents an acidic amino acid; Y represents an alkaline amino acid; α is 0 to 2 neutral amino acid(s); β represents 0 to 2 neutral amino acid(s); γ represents 1 to 10 neutral amino acid(s); n is 1 to 3. With regard to the use of the strong secretory signal peptide enhancing small peptide motifs of the present invention, it is a method for constructing a vector enhancing the secretion ability of common signal peptides to improve the secretory expression of exogenous proteins.

摘要翻译： 本发明属于蛋白质工程和基因工程领域，涉及强分泌信号肽增强小肽基序及其用途。 本发明的强分泌信号肽增强小肽基序具有下式的氨基酸序列：M（αXβYγ/αYβXγ）n，其中X代表酸性氨基酸; Y代表碱性氨基酸; α是0至2个中性氨基酸; β代表0至2个中性氨基酸; γ代表1至10个中性氨基酸; n是1至3.关于本发明的强分泌信号肽增强小肽基序的用途，它是构建增强常见信号肽分泌能力的载体的方法，以改善外源蛋白质的分泌表达 。

公开(公告)号：EP3215622A1

公开(公告)日：2017-09-13

申请号：EP15798540.9

申请日：2015-11-06

申请人： University Of Kent

发明人： ROBINSON, Colin

IPC分类号： C12N15/62 ,  C12N15/70 ,  C12N1/20 ,  C12P21/02

CPC分类号： C12N15/70 ,  C07K14/245 ,  C07K2319/034 ,  C12N1/20 ,  C12N9/16 ,  C12N15/62 ,  C12N15/625 ,  C12P21/02

摘要： The present invention resides in a method for the manufacture of a disulphide-requiring biopharmaceutical having an element of at least tertiary structure using wild type E. coli.

摘要翻译： 本发明在于使用野生型大肠杆菌制造具有至少三级结构的元件的需要二硫化物的生物药物的方法。

公开(公告)号：EP2078040B1

公开(公告)日：2017-06-28

申请号：EP07874106.3

申请日：2007-10-31

申请人： Capon, Daniel J.

发明人： Capon, Daniel J.

IPC分类号： A61K47/50 ,  A61K51/10 ,  C12N15/62

CPC分类号： C12N15/625 ,  A61K47/6835 ,  A61K47/6875 ,  A61K51/1084 ,  C07K2317/52 ,  C07K2319/30 ,  C12N15/62

公开(公告)号：EP2649178B1

公开(公告)日：2017-03-15

申请号：EP11791602.3

申请日：2011-12-09

申请人： Institut Pasteur

发明人： DESPRES, Philippe ,  PAULOUS, Sylvie ,  CRUBLET, Elodie

IPC分类号： C12N9/10 ,  C12N15/62

CPC分类号： C12N15/625 ,  C07K2319/00 ,  C07K2319/055 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/50 ,  C12N5/0601 ,  C12N5/0693 ,  C12N9/1007 ,  C12N9/96 ,  C12N15/85 ,  C12N2015/8518 ,  C12N2510/02 ,  C12P21/02 ,  C12Y201/01063 ,  Y02A50/51 ,  Y02A50/53 ,  Y02A50/60

摘要： The present invention relates to a novel enhancer of protein production in host cells. It discloses a vector for expressing recombinant proteins in these cells, comprising a nucleotide sequence encoding a) a secretion peptidic signal, b) a 6-methylguanine-DNA-methyltransferase enzyme (MGMT, EC 2.1.1.63), a mutant or a catalytic domain thereof, and c) a recombinant protein. Said MGMT enzyme is preferably the so-called SNAP protein.

摘要翻译： 本发明涉及一种在宿主细胞中产生蛋白质的新型增强子。 它公开了用于在这些细胞中表达重组蛋白质的载体，其包含编码a）分泌肽信号的核苷酸序列，b）6-甲基鸟嘌呤-DNA-甲基转移酶（MGMT，EC2.1.1.63），突变体或催化结构域 和c）重组蛋白。 所述MGMT酶优选为所谓的SNAP蛋白。

公开(公告)号：EP3000823A4

公开(公告)日：2017-02-22

申请号：EP14791458

申请日：2014-04-25

申请人： NANJING UNIV OF TECH

发明人： HE BINGFANG ,  CHEN WENHUA ,  MI LAN ,  WU SHANSHAN ,  ZHU YUN ,  CHEN SHANSHAN

IPC分类号： C07K5/103 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C12N1/21 ,  C12N9/24 ,  C12N9/42 ,  C12N15/11 ,  C12N15/70

CPC分类号： C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07K2319/02 ,  C12N9/2402 ,  C12N9/2454 ,  C12N15/625 ,  C12P21/02 ,  C12Y302/01 ,  C12Y302/01011

摘要： The present invention belongs to protein engineering and genetic engineering fields, relating to a strong secretory signal peptide enhancing small peptide motifs and the use thereof. The strong secretory signal peptide enhancing small peptide motifs of the present invention have the amino acid sequence of the following formula: M (±X²Y³/±Y²X³) n , wherein X represents an acidic amino acid; Y represents an alkaline amino acid; ± is 0 to 2 neutral amino acid(s); ² represents 0 to 2 neutral amino acid(s); ³ represents 1 to 10 neutral amino acid(s); n is 1 to 3. With regard to the use of the strong secretory signal peptide enhancing small peptide motifs of the present invention, it is a method for constructing a vector enhancing the secretion ability of common signal peptides to improve the secretory expression of exogenous proteins.

摘要翻译： 本发明属于蛋白质工程和基因工程领域，涉及强烈的分泌信号肽提高小肽基序及其用途。 强分泌信号肽提高了本发明的小肽基序具有的氨基酸序列具有下式：M（±X²Y³/±Y²X³）N酸性氨基酸的worin X darstellt; ÿdarstellt碱性氨基酸; ±为0至2的中性氨基酸（一个或多个）; ²darstellt 0-2中性氨基酸（一个或多个）; ³darstellt 1至10的中性氨基酸（一个或多个）; n为1至3.关于使用强分泌信号肽提高了本发明的小的肽基序，它是用于构建载体增强，以提高外源蛋白的分泌表达公共信号肽的分泌能力的方法 ，