公开(公告)号：EP0154522A3

公开(公告)日：1988-11-02

申请号：EP85301390

申请日：1985-02-28

申请人： TECHNICON INSTRUMENTS CORPORATION (a New York corporation)

发明人： Ornstein, Leonard ,  Kim, Young Ran

IPC分类号： G01N33/50 ,  G01N33/96

CPC分类号： G01N33/48 ,  Y10T436/101666 ,  Y10T436/107497

摘要： A one-step method (and sphering agent/fixing agent useful therein) for treating mammalian red blood cells in a sample which can be effectively measured electrooptically for determination of red blood cell volumes, which comprises treating an anticoagulated whole blood sample with a single aqueous sphering agent/fixing agent reagent at a temperature in the range of 4°C to 37°C over a reaction period of up to 180 seconds employing a whole blood dilution factor of from 1:300 to 1:4,000 wherein said ona step, single reagent treatment provides isovoluments sphering of the erythrocytes in the semple independent of the concentration of said erythrocytes in the whole Diood sample and without inducing lysis of said erychwocytes in the resulting diluted semple. The preferred sptering agent is an alkali metal salt of an alkyl sulfate irr an amount 0.5 to 3.0 mg %, and the preferred fixing agent is an aldehyde in an amount from 0.05 to 0.3%.

公开(公告)号：EP0170345A3

公开(公告)日：1987-03-25

申请号：EP85302094

申请日：1985-03-26

申请人： TECHNICON INSTRUMENTS CORPORATION (a New York corporation) ,  THE MOUNT SINAI SCHOOL OF MEDICINE

发明人： Martin, Grace E. ,  Kanter, Robert J. ,  Kim, Young Ran ,  Ornstein, Leonard ,  Ansley, Hudson R.

IPC分类号： G01N33/48 ,  G01N15/14

CPC分类号： G01N33/56972

摘要： A flow cytometry method reproducibly detects and counts a lymphocyte population of interest in a leukocyte suspension or whole blood sample in which the red cells are subsequently lysed. The suspension (or sample) is combined with a reagent comprising a primary antibody, either native, carrying an attached enzyme or biotin or other label, and a fixative reagent, in either order. Where the enzyme is not attached, an enzyme is coupled specifically to the primary antibody. The fixed suspension is reacted with a color- producing enzyme-cytochemical reagent. The suspension, now including stained and unstained fixed cells, is passed through a flow cytometer and the cells are characterized and counted on the basis of their light-scattering and light-absorbing properties.

公开(公告)号：EP0073554B1

公开(公告)日：1985-03-13

申请号：EP82302524.2

申请日：1982-05-18

申请人： TECHNICON INSTRUMENTS CORPORATION (a New York corporation)

发明人： Ornstein, Leonard ,  Kim, Young Ran

IPC分类号： G01N33/50 ,  G01N33/48

CPC分类号： G01N33/96 ,  G01N2496/05 ,  Y10T436/101666 ,  Y10T436/106664

公开(公告)号：EP0073554A1

公开(公告)日：1983-03-09

申请号：EP82302524.2

申请日：1982-05-18

申请人： TECHNICON INSTRUMENTS CORPORATION (a New York corporation)

发明人： Ornstein, Leonard ,  Kim, Young Ran

IPC分类号： G01N33/50 ,  G01N33/48

CPC分类号： G01N33/96 ,  G01N2496/05 ,  Y10T436/101666 ,  Y10T436/106664

摘要： Whole blood is treated to sphere, and optionally also fix, red blood cells in order to be able better to determine their volume and (optionally) number, by treating the blood with (a) an isotonic solution of a sphering agent, and (b) an isotonic solution of a sphering agent and a protein, to provide a weight ratio of protein to sphering agent of from 20:1 to 70:1, and a final concentration of sphering agent of from 2 to 10 mg/100 ml. In a modification of this procedure, use is made of the protein endogenous to the blood and step (b) is replaced by (c) treating the mixture from step (a) with an isotomic solution of a fixing agent such as glutaraldehyde. Reagent compositions comprising a sphering agent and a protein, for use in the above method, are also described.

摘要翻译： 将全血治疗为球体，并任选地还可以固定红细胞，以便通过用（a）等离子体试剂的等渗溶液处理血液，并且（b ）球形剂和蛋白质的等渗溶液，以提供蛋白质与球形剂的重量比为20:1至70:1，最终浓度为2至10mg / 100ml的球形剂。 在该过程的修改中，使用血液内源性的蛋白质，步骤（b）被（c）用固定剂例如戊二醛的等分溶液处理步骤（a）的混合物代替。 还描述了用于上述方法的包含球形剂和蛋白质的试剂组合物。