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    CHEMICALLY MODIFIED MUTANT ENZYMES AND METHODS FOR PRODUCING THEM AND SCREENING THEM
    1.
    发明授权
    CHEMICALLY MODIFIED MUTANT ENZYMES AND METHODS FOR PRODUCING THEM AND SCREENING THEM 有权
    为筛选酶方法的化学修饰的酶和生产

    公开(公告)号：EP1064019B1

    公开(公告)日：2010-11-03

    申请号：EP99903240.2

    申请日：1999-01-21

    申请人： THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO

    发明人： JONES, J., Bryan ,  PLETTNER, Erika

    IPC分类号： C12N15/00 ,  C12N9/14 ,  C12N9/56 ,  C12N9/16 ,  C12N9/48 ,  C11D3/00 ,  D06M16/00 ,  A61K38/48 ,  A61K38/47 ,  A61K38/46 ,  A61K38/43

    CPC分类号： C12Y304/21062 ,  C11D3/386 ,  C12N9/54

    摘要： The present invention relates to method for screening chemically modified mutant enzymes for amidase and/or esterase activity. This method includes providing a chemically modified mutant enzyme with one or more amino acid residues from an enzyme being replaced by cysteine residues, where at least some of the cysteine residues are modified by replacing thiol hydrogen in the cysteine residues with a thiol side chain, contacting the chemically modified mutant enzyme with a substrate for an amidase and/or a substrate for an esterase and determining whether the chemically modified mutant enzyme exhibits amidase and/or esterase activity. The presentinvention also relates to chemically modified mutant enzymes and a method for producing them where one or more amino acid residues from an enzyme are replaced by cysteine residues, and the cysteine residues are modified by replacing at least some of the thiol hydrogen in the cysteine residue with a thiol side chain to form the chemically modified mutant enzyme. The thiol side chain is selected from the group consisting of -SCH2(p-CH3-C6H4), -SCH2(p-OCH3-C6H4), -SCH2(p-CF3-C6H4), and -SCH2(2,4-diNO2-C6H3).

    CHEMICALLY MODIFIED PROTEINS WITH A CARBOHYDRATE MOIETY
    3.
    发明授权
    CHEMICALLY MODIFIED PROTEINS WITH A CARBOHYDRATE MOIETY 有权
    与碳水化合物集团化学修饰的蛋白质

    公开(公告)号：EP1093459B1

    公开(公告)日：2008-09-10

    申请号：EP99937203.0

    申请日：1999-07-02

    申请人： THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO

    发明人： JONES, J., Bryan ,  DAVIS, Benjamin, G.

    IPC分类号： C07K1/107 ,  C07K14/00 ,  C12N9/00 ,  C12N9/54 ,  C12N9/96 ,  C12N15/00 ,  C12N15/52 ,  C12N15/57 ,  C12Q1/25 ,  C12Q1/37

    CPC分类号： B82Y5/00 ,  C12N9/54

    摘要： The present invention relates to a chemically modified mutant protein including a cysteine residue substituted for a residue other than cysteine in a precursor protein, the substituted cysteine residue being subsequently modified by reacting the cysteine residue with a glycosylated thiosulfonate. Also, a method of producing the chemically modified mutant protein is provided. The present invention also relates to a glycosylated methanethiosulfonate. Another aspect of the present invention is a method of modifying the functional characteristics of a protein including providing a protein and reacting the protein with a glycosylated methanethiosulfonate reagent under conditions effective to produce a glycoprotein with altered functional characteristics as compared to the protein. In addition, the present invention relates to methods of determining the structure-function relationships of chemically modified mutant proteins.