公开(公告)号：EP1021544A2

公开(公告)日：2000-07-26

申请号：EP98952184.4

申请日：1998-10-08

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： PLETNEV, Alexander ,  MEN, Ruhe ,  CHANOCK, Robert ,  LAI, Ching-Juh

IPC分类号： C12N15/40 ,  C07K14/18 ,  C12N7/04 ,  A61K39/295

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/5254 ,  C12N7/00 ,  C12N2770/24122 ,  C12N2770/24161 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/396

摘要： A live, attenuated chimeric virus vaccine against tick-borne encephalitis virus comprising the preM and E structural genes of the tick-borne encephalitis Langat virus and the non-structural genes of the mosquito-borne dengue virus. The live chimeric vaccine was administered intraperitoneally and exhibited complete attenuation in mice while at the same time providing protection against subsequent challenge with the virulent parental Langat virus.

公开(公告)号：EP1261701A2

公开(公告)日：2002-12-04

申请号：EP01910561.8

申请日：2001-02-09

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： PLETNEV, Alexander ,  CHANOCK, Robert

IPC分类号： C12N15/00

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K2039/5254 ,  C12N7/00 ,  C12N2770/24122 ,  C12N2770/24134 ,  C12N2770/24161 ,  C12N2770/24164

摘要： Provided are full-length cDNA clones of Langat tick-borne flavivirus. In particular, the strains TP21 and E5 were obtained as full-length clones in single plasmids. Production of virions from cloned full length cDNA resulted in variants wih reduced neurovirulence. Deletions in the 3' NCR also decreased neurovirulence. The attenuated variants of Langat tick-borne flavivirus may be used for vaccination purposes.