公开(公告)号：EP0604566A1

公开(公告)日：1994-07-06

申请号：EP92920708.0

申请日：1992-09-18

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by THE SECRETARY of the DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： LAI, Ching-Juh ,  BRAY, Michael ,  PLETNEV, Alexander, G. ,  MEN, Ruhe ,  PETHEL, Michele

IPC分类号： C12N15 ,  A61K31 ,  A61K39 ,  A61P31 ,  C07K14 ,  C12N1 ,  C12N7 ,  C12R1

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/522 ,  A61K2039/5256 ,  A61K2039/5258 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2770/24022 ,  C12N2770/24122 ,  C12N2770/24134 ,  C12N2770/24161 ,  C12N2770/24162 ,  C12N2770/24322 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/39 ,  Y02A50/396

摘要： The invention includes a chimeric virus for use in a vaccine preparation having a genome comprising nucleic acid sequences encoding at least one structural protein from one flavivirus and nucleic acid sequences encoding nonstructural protein from another flavivirus. The genome preferably includes mutations within the viral genome that reduce virus virulence and in a particularly preferred embodiment these vaccines are directed to flaviviruses such as dengue virus, tick-borne encephalitis virus and Japanese encephalitis virus.

公开(公告)号：EP1021544A2

公开(公告)日：2000-07-26

申请号：EP98952184.4

申请日：1998-10-08

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： PLETNEV, Alexander ,  MEN, Ruhe ,  CHANOCK, Robert ,  LAI, Ching-Juh

IPC分类号： C12N15/40 ,  C07K14/18 ,  C12N7/04 ,  A61K39/295

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/5254 ,  C12N7/00 ,  C12N2770/24122 ,  C12N2770/24161 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/396

摘要： A live, attenuated chimeric virus vaccine against tick-borne encephalitis virus comprising the preM and E structural genes of the tick-borne encephalitis Langat virus and the non-structural genes of the mosquito-borne dengue virus. The live chimeric vaccine was administered intraperitoneally and exhibited complete attenuation in mice while at the same time providing protection against subsequent challenge with the virulent parental Langat virus.

公开(公告)号：EP0604566B1

公开(公告)日：1999-11-17

申请号：EP92920708.2

申请日：1992-09-18

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by THE SECRETARY of the DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： LAI, Ching-Juh ,  BRAY, Michael ,  PLETNEV, Alexander, G. ,  MEN, Ruhe ,  CHANOCK, Robert, M.

IPC分类号： C12N15/40 ,  C12N7/01 ,  C12N1/20 ,  A61K39/12

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/522 ,  A61K2039/5256 ,  A61K2039/5258 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2770/24022 ,  C12N2770/24122 ,  C12N2770/24134 ,  C12N2770/24161 ,  C12N2770/24162 ,  C12N2770/24322 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/39 ,  Y02A50/396

摘要： The invention includes a chimeric virus for use in a vaccine preparation having a genome comprising nucleic acid sequences encoding at least one structural protein from one flavivirus and nucleic acid sequences encoding nonstructural protein from another flavivirus. The genome preferably includes mutations within the viral genome that reduce virus virulence and in a particularly preferred embodiment these vaccines are directed to flaviviruses such as dengue virus, tick-borne encephalitis virus and Japanese encephalitis virus.