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公开(公告)号:EP3191584A4
公开(公告)日:2018-07-18
申请号:EP15839739
申请日:2015-09-10
申请人: THE STATE OF ISRAEL MINISTRY OF AGRICULTURE & RURAL DEVELOPMENT AGRICULTURAL RES ORGANIZATION ARO VO
发明人: ITKIN MAXIM , DAVIDOVICH-RIKANATI RACHEL , COHEN SHAHAR , PORTNOY VITALY , DORON-FAIGENBOIM ADI , PETREIKOV MARINA , SHEN SHMUEL , TADMOR YAAKOV , BURGER YOSEF , LEWINSOHN EFRAIM , KATZIR NURIT , SCHAFFER ARTHUR A , OREN ELAD
CPC分类号: C12P33/12 , A23L2/60 , A23L27/36 , A23V2002/00 , C07J17/005 , C12N9/0071 , C12N9/0083 , C12N9/14 , C12N9/88 , C12N9/90 , C12P19/56 , C12P33/20 , C12Y114/99007 , C12Y204/02017 , C12Y402/01 , C12Y504/99033
摘要: Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), expoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT). Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol.