公开(公告)号：EP4379050A1

公开(公告)日：2024-06-05

申请号：EP22848384.8

申请日：2022-07-21

申请人： Abiochem Biotechnology (Group) Co., Ltd.

发明人： WU, Yan ,  TIAN, Zhenhua ,  WANG, Shu ,  ZHENG, Xiaofu ,  XING, Litong

IPC分类号： C12N9/10 ,  C12N15/54 ,  C12N15/63 ,  C12P19/00

CPC分类号： C12P19/56 ,  C12N9/10 ,  C12R2001/1920210501 ,  C12N15/70 ,  C12N15/74 ,  C12N15/63 ,  C12P19/00

摘要： Provided are a sucrose synthetase and the use thereof. Compared with SEQ ID NO: 11, an amino acid sequence of the sucrose synthetase contains differences in amino acid residues selected from one or more of the following residue positions: an amino acid at position 36 being V, an amino acid at position 43 being E, an amino acid at position 179 being A, an amino acid at position 395 being R, an amino acid at position 447 being P, an amino acid at position 455 being L, and an amino acid at position 654 being R; and the sucrose synthetase has an activity not lower than that of a sucrose synthetase having an amino acid sequence as shown in SEQ ID NO: 11. By means of catalyzing the synthesis of rebaudioside A, rebaudioside D or rebaudioside M by using a glucosyltransferase and the sucrose synthetase in a combined manner, a cascade reaction is achieved, thereby providing a variety of possibilities for the selection of raw materials.

公开(公告)号：EP2806754B1

公开(公告)日：2018-10-24

申请号：EP13700929.6

申请日：2013-01-23

申请人： DSM IP Assets B.V.

发明人： KUMAR, Manoj ,  VAN DEN BERG, Marco Alexander ,  BOER, Viktor Marius ,  LAWRENCE, Adam G. ,  LEHMANN, Martin ,  MEIJRINK, Bernard ,  SAGT, Cornelis Maria Jacobus ,  TRUEHART, Joshua ,  WU, Liang ,  ZWARTJENS, Priscilla

IPC分类号： C12N9/02 ,  C12N9/10 ,  C12N9/90 ,  A23L2/60 ,  C12P5/00 ,  C07H15/256 ,  C12P19/56 ,  C12N15/52 ,  A23K20/121 ,  A23K20/163 ,  A23K20/10 ,  A23L27/30 ,  C12N9/88

CPC分类号： C12P19/56 ,  A23K20/10 ,  A23K20/121 ,  A23K20/163 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C07H15/256 ,  C12N9/0073 ,  C12N9/1051 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P5/007 ,  C12Y114/13078 ,  C12Y204/01035 ,  C12Y402/03019 ,  C12Y505/01013

摘要： The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.

公开(公告)号：EP3387136A1

公开(公告)日：2018-10-17

申请号：EP16822120.8

申请日：2016-12-09

申请人： Evolva SA

发明人： DYEKJAER, Jane Dannow ,  HOUGHTON-LARSEN, Jens ,  DOUCHIN, Veronique

IPC分类号： C12N15/81 ,  C07K14/395 ,  C12N9/10 ,  C12N9/90 ,  C12N15/82 ,  C12P15/00 ,  A23L27/30

CPC分类号： C12N15/81 ,  A23L27/36 ,  C07K14/395 ,  C12N9/10 ,  C12N9/1048 ,  C12N9/90 ,  C12N15/52 ,  C12N15/82 ,  C12P15/00 ,  C12P19/56

摘要： The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors.

公开(公告)号：EP2862927B1

公开(公告)日：2018-10-17

申请号：EP13797812.8

申请日：2013-05-29

申请人： Suntory Holdings Limited

发明人： ONO, Eiichiro

IPC分类号： C12N15/09 ,  A01H5/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/10 ,  C12P19/18 ,  C12P19/56 ,  C12N15/82 ,  C12P15/00

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12P15/00 ,  C12P19/56 ,  C12Y204/01

摘要： [Problem to be Solved] The present invention aims to provide steviol glycosyltransferase and a method for producing a steviol glycoside using this enzyme. [Means to Solve the Problem] The present invention provides steviol glycosyltransferase and a method for producing a steviol glycoside using this enzyme. The present invention provides a transformant transformed with a gene for steviol glycosyltransferase and a method for preparing such a transformant.

公开(公告)号：EP3191584A4

公开(公告)日：2018-07-18

申请号：EP15839739

申请日：2015-09-10

申请人： THE STATE OF ISRAEL MINISTRY OF AGRICULTURE & RURAL DEVELOPMENT AGRICULTURAL RES ORGANIZATION ARO VO

发明人： ITKIN MAXIM ,  DAVIDOVICH-RIKANATI RACHEL ,  COHEN SHAHAR ,  PORTNOY VITALY ,  DORON-FAIGENBOIM ADI ,  PETREIKOV MARINA ,  SHEN SHMUEL ,  TADMOR YAAKOV ,  BURGER YOSEF ,  LEWINSOHN EFRAIM ,  KATZIR NURIT ,  SCHAFFER ARTHUR A ,  OREN ELAD

IPC分类号： C12N9/02 ,  C12N9/14 ,  C12N9/90 ,  C12N15/54 ,  C12N15/79 ,  C12P19/18 ,  C12P19/56

CPC分类号： C12P33/12 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C07J17/005 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/14 ,  C12N9/88 ,  C12N9/90 ,  C12P19/56 ,  C12P33/20 ,  C12Y114/99007 ,  C12Y204/02017 ,  C12Y402/01 ,  C12Y504/99033

摘要： Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), expoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT). Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol.

公开(公告)号：EP3334819A1

公开(公告)日：2018-06-20

申请号：EP16756990.4

申请日：2016-08-15

申请人： DSM IP Assets B.V.

发明人： BOER, Viktor, Marius ,  ZWARTJENS, Priscilla ,  VAN DEN BERG, Eric

IPC分类号： C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12P19/56 ,  C07K14/39

CPC分类号： C12P19/56 ,  C07H15/24 ,  C07K14/39 ,  C12N9/1051 ,  C12Y204/01017

摘要： A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 35 or SEQ ID NO: 38 or an amino acid sequence having at least about 50% sequence identity to either thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 35 or SEQ ID NO: 38 or an amino acid sequence having at least about 50% sequence identity to either thereto.

公开(公告)号：EP3303553A1

公开(公告)日：2018-04-11

申请号：EP16804137.4

申请日：2016-05-27

申请人： Cargill, Incorporated ,  Evolva

发明人： ANDERSON, James C. ,  ASENSIO, Manuel Quiros ,  CARLSEN, Simon ,  CARLSON, Ting Liu ,  DOUCHIN, Veronique ,  FOSMER, Arlene M. ,  SMITS, Hans Peter

IPC分类号： C12N1/19 ,  C12P19/00

CPC分类号： C12P19/56 ,  A23L27/36 ,  A23V2002/00 ,  C07H15/256

摘要： Disclosed are methods for producing steviol glycosides, such as rebaudioside D and rebaudioside M, using engineered yeast. In some embodiments, the methods include fermenting with a yeast at a high pH such as of about 5.8 or greater. In some embodiments, the methods can be carried out by first growing the yeast at a lower first pH, and then adjusting the pH to a higher pH.

公开(公告)号：EP3004127A4

公开(公告)日：2018-01-03

申请号：EP14804243

申请日：2014-05-28

申请人： PURECIRCLE SDN BHD ,  THE COCA-COLA COMPANY

发明人： MARKOSYAN AVETIK ,  PRAKASH INDRA ,  BUNDERS CYNTHIA ,  SONI PANKAJ ,  CYRILLE JARRIN ,  BADIE AURÉLIEN ,  TER HALLE ROBERT

IPC分类号： C12P19/56 ,  A23L2/60 ,  A23L27/30 ,  C07H1/06 ,  C07H15/256 ,  C12N9/10

CPC分类号： C12P19/56 ,  A23L2/60 ,  A23L27/33 ,  A23V2002/00 ,  A24B15/10 ,  A24B15/302 ,  C07H15/256 ,  Y02P20/582 ,  A23V2250/258

摘要： Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and M are described. The methods include utilizing recombinant microorganisms for converting various staring compositions to target steviol glycosides. In addition, novel steviol glycosides reb D2 and reb M2 are disclosed, as are methods of preparing the same. The highly purified rebaudiosides are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.

公开(公告)号：EP3204506A1

公开(公告)日：2017-08-16

申请号：EP15778289.7

申请日：2015-10-08

申请人： DSM IP Assets B.V.

发明人： BERG, VAN DEN, Marco Alexander ,  MADERN, Marcella Katharina ,  WINTER, Remko Tsjibbe

IPC分类号： C12P19/18 ,  C12N15/67

CPC分类号： C12P19/56 ,  A23L27/33 ,  A23L27/36 ,  A23V2002/00 ,  C12N15/52 ,  C12P19/18 ,  C12Y106/02004 ,  C12Y114/13078 ,  C12Y114/13079 ,  C12Y204/00 ,  C12Y402/03019 ,  C12Y505/01013

摘要： a. contacting said composition comprising steviol glycosides with a recombinant microorganism, a cell free extract derived from such a microorganism or an enzyme preparation derived from either thereof; and b. thereby to increase the degree of glycosylation of the composition comprising steviol glycosides, wherein the recombinant microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity;a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; a polypeptide having kaurenoic acid 13-hydroxylase activity; and one or more polypeptides having UDP-glucosyltransferase activity whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least one steviol glycoside. The present invention also relates to a composition comprising steviol glycosides obtainable by such a method.

摘要翻译： 本发明涉及用于提高包含甜菊醇糖苷的组合物的糖基化程度的方法，该方法包括：a。 使所述包含甜菊醇糖苷的组合物与重组微生物，源自该微生物的无细胞提取物或源自其任一的酶制剂接触; 和b。 从而增加包含甜菊醇糖苷的组合物的糖基化程度，其中所述重组微生物包含一种或多种编码以下核苷酸序列的核苷酸序列：具有对焦酰基焦磷酸合酶活性的多肽;具有内 - 胡安烯合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 具有kaurenoic酸13-羟化酶活性的多肽; 和一种或多种具有UDP-葡糖基转移酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物产生至少一种甜菊醇糖苷的能力。 本发明还涉及包含通过这种方法可获得的甜菊醇糖苷的组合物。

公开(公告)号：EP3201211A1

公开(公告)日：2017-08-09

申请号：EP15845748.1

申请日：2015-10-02

申请人： Conagen Inc.

发明人： MAO, Guohong ,  YU, Xiaodan

IPC分类号： C07H15/24

CPC分类号： C07H15/24 ,  A23L2/60 ,  A23L27/36 ,  A23L33/10 ,  A23V2002/00 ,  C07H1/00 ,  C07H15/256 ,  C07K2319/00 ,  C12N9/1051 ,  C12N9/1062 ,  C12P19/18 ,  C12P19/56 ,  C12Y204/01 ,  C12Y204/01013 ,  C12Y204/01017

摘要： Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).