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    METHOD FOR DETECTING TARGET BASE SEQUENCE USING COMPETITIVE PRIMER
    4.
    发明公开
    METHOD FOR DETECTING TARGET BASE SEQUENCE USING COMPETITIVE PRIMER 有权
    VERFAHREN ZUR ERKENNUNG VON ZIELBASISSEQUENZEN MITHILFE KONKURRIERENDER PRIMER

    公开(公告)号：EP2551356A4

    公开(公告)日：2013-09-11

    申请号：EP11759408

    申请日：2011-03-23

    申请人： TOPPAN PRINTING CO LTD

    发明人： MAKINO YOICHI ,  YAMANE AKIO ,  NAKAYAMA MASATO

    IPC分类号： C12Q1/68 ,  C12N15/09

    CPC分类号： C12Q1/6869 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2531/119 ,  C12Q2537/161 ,  C12Q2531/143 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2525/185 ,  C12Q2525/204 ,  C12Q2535/125 ,  C12Q2549/101 ,  C12Q2563/107 ,  C12Q2565/101

    摘要： Disclosed is a method of detecting a target base sequence having a polymorphic base, the method including: (a) a step of adding to a nucleic acid sample having a target nucleic acid that includes a base sequence including the target base sequence: at least one type of detection primer, at least one type of competitive primer, and at least one type of common primer; (b) a step of annealing the detection primer and the competitive primer to the target nucleic acid in a competitive manner, thereby synthesizing an extension product A; (c) a step of annealing the common primer to the extension product A obtained in the step (b) or in the following step (d), thereby synthesizing an extension product B; (d) a step of annealing the detection primer or the competitive primer to the extension product B obtained in the previous step (c), thereby synthesizing the extension product A; and (e) a step of detecting the extension product A or the extension product B.

    摘要翻译： 公开了一种检测具有多态性碱基的靶碱基序列的方法，该方法包括：（a）向具有靶核酸的核酸样品添加步骤，所述靶核酸包括含有靶碱基序列的碱基序列：至少一个 类型的检测引物，至少一种类型的竞争性引物和至少一种类型的通用引物; （b）以竞争的方式将检测用引物和竞争性引物与靶核酸退火而合成延伸产物A的工序; （c）将通用引物退火至在步骤（b）或在随后的步骤（d）中获得的延伸产物A的步骤，由此合成延伸产物B; （d）将检测用引物或竞争性引物退火至前一步骤（c）中获得的延伸产物B，从而合成延伸产物A的步骤; 和（e）检测延伸产品A或延伸产品B的步骤。