公开(公告)号：EP2468850A1

公开(公告)日：2012-06-27

申请号：EP10809959.9

申请日：2010-08-17

申请人： Takara Bio, Inc.

发明人： TANABE, Masashige ,  ISONO, Tomomi ,  ENOKI, Tatsuji

IPC分类号： C12N5/071 ,  C12N5/07

CPC分类号： A01N1/0221

摘要： A method for cryopreservation of cells characterized by the use of a solution containing, as essential components, a sodium salt, a potassium salt, a saccharide, a cryoprotectant, and a hydrogencarbonate salt and/or a carbonate salt, the solution further optionally containing a component selected from the group consisting of proteins, magnesium salts, and calcium salts. According to the present invention, a method for cryopreservation of cells capable of maintaining a high viability rate after thawing, and a cryopreservation solution used in the cryopreservation are provided. In addition, by using the method for cryopreservation, the cells can be preserved in a state of maintaining a high viability rate even after thawing, and also maintaining their physiological functions; further, a cell washing step after freezing and thawing is unnecessary, thereby making it very useful in the fundamental researches of cells and the application studies on the medical treatment.

摘要翻译： 一种用于冷冻保存细胞的方法，其特征在于使用含有钠盐，钾盐，糖类，冷冻保护剂和碳酸氢盐和/或碳酸盐作为必要组分的溶液，该溶液还任选地含有 选自蛋白质，镁盐和钙盐的组分。 根据本发明，提供了一种在解冻后能够保持高活力率的细胞的低温保存方法和用于冷冻保存的冷冻保存溶液。 另外，通过使用冷冻保存方法，即使在解冻后也可以将细胞保持在维持高生存率的状态，并且还保持其生理功能; 此外，不需要冷冻和解冻后的细胞洗涤步骤，从而使其在细胞的基础研究和医疗应用研究中非常有用。