公开(公告)号：EP2295538A1

公开(公告)日：2011-03-16

申请号：EP09794433.4

申请日：2009-07-07

申请人： Takara Bio, Inc.

发明人： ENOKI, Tatsuji ,  IWAMOTO, Fumiko ,  NISHIE, Toshikazu ,  MARUI, Takahiro ,  TAKASHIMA, Fuyuko ,  KATO, Ikunoshin

IPC分类号： C12N5/07 ,  C12N5/10 ,  C12N15/09

CPC分类号： C12N5/0696 ,  C12N2501/405 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/608 ,  C12N2501/727 ,  C12N2510/00 ,  C12N2533/52 ,  C12N2740/15043 ,  C12N2740/15071

摘要： The present invention relates to a method for production of a cell population containing a pluripotent stem cell, said method comprising a step of treating a somatic cell which has been contacted with nuclear reprogramming factors under nutrient-starved condition, and/or a step of treating the somatic cell with an agent capable of arresting cell cycle. The present invention allows induction and growth of pluripotent stem cells at high frequency, and it also allows production of pluripotent stem cells with high efficiency. The nuclear reprogramming factors to be used may be any selected from the group consisting of OCT4, SOX2, c-MYC, KLF4, NANOG and LIN28.

摘要翻译： 本发明涉及含有多能干细胞的细胞群的生产方法，所述方法包括处理在营养缺乏条件下与核重编程因子接触的体细胞的步骤，和/或处理 具有能够阻止细胞周期的药剂的体细胞。 本发明允许高倍数的多能干细胞的诱导和生长，并且还能够以高效率生产多能干细胞。 要使用的核重编程因子可以选自OCT4，SOX2，c-MYC，KLF4，NANOG和LIN28中的任何一种。

公开(公告)号：EP2295538B1

公开(公告)日：2015-10-21

申请号：EP09794433.4

申请日：2009-07-07

申请人： Takara Bio Inc.

发明人： ENOKI, Tatsuji ,  IWAMOTO, Fumiko ,  NISHIE, Toshikazu ,  MARUI, Takahiro ,  TAKASHIMA, Fuyuko ,  KATO, Ikunoshin

IPC分类号： C12N5/07 ,  C12N5/10 ,  C12N15/09

CPC分类号： C12N5/0696 ,  C12N2501/405 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/608 ,  C12N2501/727 ,  C12N2510/00 ,  C12N2533/52 ,  C12N2740/15043 ,  C12N2740/15071