公开(公告)号：EP4105337A1

公开(公告)日：2022-12-21

申请号：EP22175488.0

申请日：2015-09-09

申请人： The Broad Institute, Inc. ,  President and Fellows of Harvard College ,  Massachusetts Institute Of Technology

发明人： REGEV, Aviv ,  MACOSKO, Evan Zane ,  MCCARROLL, Steven Andrew ,  SHALEK, Alexander K ,  BASU, Anindita ,  FORD, Christopher B ,  PARK, Hongkun ,  WEITZ, David A

IPC分类号： C12Q1/68 ,  C12N15/10

摘要： The present invention generally relates to a combination of molecular barcoding and emulsion-based microfluidics to isolate, lyse, barcode, and prepare nucleic acids from individual cells in a high-throughput manner.

公开(公告)号：EP3224380A1

公开(公告)日：2017-10-04

申请号：EP15810708.6

申请日：2015-11-23

申请人： The Broad Institute Inc. ,  President and Fellows of Harvard College

发明人： MCCARROLL, Steven Andrew ,  GENOVESE, Giulio

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  A61K35/14 ,  A61K35/28 ,  C12Q2600/118 ,  C12Q2600/156 ,  G01N2333/91017

摘要： The present invention relates to clonal expansion of somatic cells in subjects, and acquired selective advantage of cell clones during the lifetime of a subject. In particular, the invention relates to methods for predicting the development of cancer based on the observation of specific genetic mutations in somatic cell clones, as well as to methods for treating or preventing cancer in a subject, in which clonal expansion of cells comprising specific modifications is observed.

摘要翻译： 本发明涉及受试者中体细胞的克隆性扩增，并且在受试者的一生中获得细胞克隆的选择性优势。 具体而言，本发明涉及基于观察体细胞克隆中的特定基因突变预测癌症发展的方法，以及用于治疗或预防受试者中癌症的方法，其中包含特异性修饰的细胞克隆扩增 被观察到。