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公开(公告)号：EP2638393B1
公开(公告)日：2019-03-06
申请号：EP11839117.6
申请日：2011-11-09
申请人： The General Hospital Corporation , Massachusetts Institute of Technology , The Board of Trustees of the University of Illinois , Daktari Diagnostics, Inc.
发明人： WATKINS, Nicholas , BASHIR, Rashid , RODRIGUEZ, William , CHENG, Xuanhong , TONER, Mehmet , CHEN, Grace , OPPENHEIMER, Aaron
IPC分类号： G01N33/53 , G01N33/52 , G01N21/17 , C12M1/34 , G01N15/12 , G01N33/569 , G01N33/543 , G01N15/10 , G01N15/14
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公开(公告)号：EP2638393A2
公开(公告)日：2013-09-18
申请号：EP11839117.6
申请日：2011-11-09
申请人： The General Hospital Corporation , Massachusetts Institute of Technology , The Board of Trustees of the University of Illinois , Daktari Diagnostics, Inc.
发明人： WATKINS, Nicholas , BASHIR, Rashid , RODRIGUEZ, William , CHENG, Xuanhong , TONER, Mehmet , CHEN, Grace , OPPENHEIMER, Aaron
CPC分类号： G01N27/02 , B01L2200/0652 , B01L2300/0877 , C12M41/36 , G01N15/12 , G01N15/1245 , G01N33/56972 , G01N2015/1006 , G01N2015/1486
摘要： This disclosure relates to methods and devices to count particles of interest, such as cells. The methods include obtaining a fluid sample that may contain particles of interest; counting all types of particles in a portion of the sample using a first electrical differential counter to generate a first total; removing any particles of interest from the portion of the fluid sample; counting any particles remaining in the portion of the fluid sample using a second electrical differential counter after the particles of interest are removed to generate a second total; and calculating a number of particles of interest originally in the fluid sample by subtracting the second total from the first total, wherein the difference is the number of particles of interest in the sample. These methods and related devices can be used, for example, to produce a robust, inexpensive diagnostic kit for CD4+ T cell counting in whole blood samples.
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公开(公告)号：EP2156376B1
公开(公告)日：2013-09-25
申请号：EP08743095.5
申请日：2008-04-21
发明人： TONER, Mehmet , BASHIR, Rashid , CHENG, Xuanhong , DEMIRCI, Utkan , IRIMIA, Daniel , RODRIGUEZ, William , YANG, Liju , ZAMIR, Lee
CPC分类号： G01N33/5005 , G01N15/1031 , G01N15/1056 , G01N15/1484 , G01N33/84 , G01N2015/1006 , G01N2015/1062 , Y02A50/58
摘要： The invention features methods of quantifying cells in a sample by lysing the cells followed by the measurement of at least one intracellular component. Methods of the invention are especially useful for quantifying small numbers of cells, e.g., over a large surface area or volume compared to the cell size. In a preferred embodiment, methods of the invention are performed using a microfluidic device.
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公开(公告)号：EP2002262A2
公开(公告)日：2008-12-17
申请号：EP07753420.4
申请日：2007-03-15
IPC分类号： G01N33/574
CPC分类号： G01N33/56972 , G01N33/56966 , Y10S436/824
摘要： The invention features methods, devices, and kits for the isolation of analytes (e.g., a cell). A sample containing a desired analyte is introduced into a microfluidic device containing moieties that bind the desired analyte. A shear stress is applied that is great enough to prevent binding of undesired analytes and low enough to allow binding of the analyte of interest. Once bound, the desired analytes can be analyzed (e.g., counted). The invention also features methods for determining a shear stress for isolating a desired analyte.
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公开(公告)号：EP2002262B1
公开(公告)日：2016-06-22
申请号：EP07753420.4
申请日：2007-03-15
CPC分类号： G01N33/56972 , G01N33/56966 , Y10S436/824
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公开(公告)号：EP2156376A1
公开(公告)日：2010-02-24
申请号：EP08743095.5
申请日：2008-04-21
发明人： TONER, Mehmet , BASHIR, Rashid , CHENG, Xuanhong , DEMIRCI, Utkan , IRIMIA, Daniel , RODRIGUEZ, William , YANG, Liju , ZAMIR, Lee
CPC分类号： G01N33/5005 , G01N15/1031 , G01N15/1056 , G01N15/1484 , G01N33/84 , G01N2015/1006 , G01N2015/1062 , Y02A50/58
摘要： The invention features methods of quantifying cells in a sample by lysing the cells followed by the measurement of at least one intracellular component. Methods of the invention are especially useful for quantifying small numbers of cells, e.g., over a large surface area or volume compared to the cell size. In a preferred embodiment, methods of the invention are performed using a microfluidic device.
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