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公开(公告)号：EP2566961B1
公开(公告)日：2018-08-22
申请号：EP11730124.2
申请日：2011-05-05
发明人： ALEKSEYEV, Viktor Yuryevich , AMIN, Neelam, S. , AUGUSTYN, Katherine , CASCAO-PEREIRA, Luis Gustavo , COLLIER, Katherine, D. , ESTELL, David, A. , KELLIS, JR., James, T. , POULOSE, Ayrookaran, J. , SOUTER, Philip Frank , WARD, Glenn Steven , YAO, Jian
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681
摘要： A method of treating and/or cleaning a surface, preferably a fabric surface comprising the steps of (i) contacting said surface with a composition in an aqueous wash liquor, said composition comprising an adjunct material and a protease variant wherein said protease variant is a variant of a parent protease said parent protease's sequence being at least 90% identical to the amino acid sequence of SEQ ID NO:1, wherein the amino acid positions of the protease variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of Bacillus amyloliquefaciens subtilisin BPN' shown in SEQ ID NO:2, wherein said protease variant comprises a mutation selected from T022A, T022R, T022Y, T022V, T022Q, T022L, T022W, and (ii) rinsing and/or drying the surface, wherein the temperature of the aqueous liquor is from 5 to 30°C.
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公开(公告)号：EP3868881A3
公开(公告)日：2021-11-03
申请号：EP20213383.1
申请日：2011-05-05
发明人： ALEKSEYEV, Viktor Yuryevich , AMIN, Neelam, S , AUGUSTYN, Katherine , CASCAO-PEREIRA, Luis Gustavo , COLLIER, Katherine, D. , ESTELL, David, A. , KELLIS JR., James, T , POULOSE, Ayrookaran, J. , SOUTER, Philip Frank , WARD, Glenn Steven , YAO, Jian
摘要： The present invention provides isolated subtilisin variants. Specifically, a subtilisin variant is a mature form having proteolytic activity and comprises an amino acid sequence comprising the combination of amino acid substitutions: T022R, S101G, S103A, V104I, A232V, and Q245R, wherein the variant is a variant of Bacillus lentus subtilisin GG36 protease comprising the amino acid sequence shown in SEQ ID NO:2, and wherein the variant has at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 2, and wherein the amino acid positions of the subtilisin variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of B. amyloliquefaciens subtilisin BPN' set forth as SEQ ID NO: 1. In addition, the present invention provides compositions comprising these subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these subtilisin variants and methods of cleaning using the cleaning compositions.
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公开(公告)号：EP3095861B1
公开(公告)日：2019-06-26
申请号：EP16177383.3
申请日：2011-05-05
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公开(公告)号：EP3418382A1
公开(公告)日：2018-12-26
申请号：EP18182799.9
申请日：2011-05-05
发明人： ALEKSEYEV, Viktor Yuryevich , AMIN, Neelam S. , AUGUSTYN, Katherine , CASCAO-PEREIRA, Luis Gustavo , COLLIER, Katherine D. , ESTELL, David A. , KELLIS, James T., Jr. , POULOSE, Ayrookaran J. , SOUTER, Philip Frank , WARD, Glenn Steven , YAO, Jian
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681
摘要： The present invention provides isolated subtilisin variants. Specifically, a subtilisin variant is a mature form having proteolytic activity and comprises an amino acid sequence comprising the combination of amino acid substitutions: T022R/A, S101G, S103A, V104I, A232V, and Q245R, wherein the variant is a variant of Bacillus lentus subtilisin GG36 protease comprising the amino acid sequence shown in SEQ ID NO:2, and wherein the variant has at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 2, and wherein the amino acid positions of the subtilisin variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of B. amyloliquefaciens subtilisin BPN' set forth as SEQ ID NO: 1. In addition, the present invention provides compositions comprising these subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these subtilisin variants and methods of cleaning using the cleaning compositions.
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公开(公告)号：EP3095861A1
公开(公告)日：2016-11-23
申请号：EP16177383.3
申请日：2011-05-05
发明人： SOUTER, Philip Frank , WARD, Glenn Steven , POULOSE, Ayrookaran Joseph , ESTELL, David A. , KELLIS Jr., James T. , COLLIER, Katherine D. , CASCAO-PEREIRA, Luis Gustavo , ALEKSEYEV, Viktor Yuryevich , AMIN, Neelam S. , YAO, Jian , AUGUSTYN, Katherine
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681
摘要： A method of treating and/or cleaning a surface, preferably a fabric surface comprising the steps of (i) contacting said surface with a composition in an aqueous wash liquor, said composition comprising an adjunct material and a protease variant wherein said protease variant is a variant of a parent protease said parent protease's sequence being at least 90% identical to the amino acid sequence of SEQ ID NO:1, wherein the amino acid positions of the protease variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of Bacillus amyloliquefaciens subtilisin BPN' shown in SEQ ID NO:2, wherein said protease variant comprises a mutation selected from T022A, T022R, T022Y, T022V, T022Q, T022L, T022W, and (ii) rinsing and/or drying the surface, wherein the temperature of the aqueous liquor is from 5 to 30°C.
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6.发明公开COMPOSITIONS AND METHODS COMPRISING SUBTILISIN VARIANTS 有权ZUSAMMENSETZUNGEN UND VERFAHREN MIT SUBTILISINVARIANTEN
公开(公告)号：EP2566961A1
公开(公告)日：2013-03-13
申请号：EP11730124.2
申请日：2011-05-05
发明人： ALEKSEYEV, Viktor Yuryevich , AMIN, Neelam, S. , AUGUSTYN, Katherine , CASCAO-PEREIRA, Luis Gustavo , COLLIER, Katherine, D. , ESTELL, David, A. , KELLIS, JR., James, T. , POULOSE, Ayrookaran, J. , SOUTER, Philip Frank , WARD, Glenn Steven , YAO, Jian
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681
摘要： A method of treating and/or cleaning a surface, preferably a fabric surface comprising the steps of (i) contacting said surface with a composition in an aqueous wash liquor, said composition comprising an adjunct material and a protease variant wherein said protease variant is a variant of a parent protease said parent protease's sequence being at least 90% identical to the amino acid sequence of SEQ ID NO:1, wherein the amino acid positions of the protease variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of Bacillus amyloliquefaciens subtilisin BPN' shown in SEQ ID NO:2, wherein said protease variant comprises a mutation selected from T022A, T022R, T022Y, T022V, T022Q, T022L, T022W, and (ii) rinsing and/or drying the surface, wherein the temperature of the aqueous liquor is from 5 to 30°C.
摘要翻译： 一种处理和/或清洁表面，优选织物表面的方法，包括以下步骤：（i）使所述表面与组合物在水性洗涤液中接触，所述组合物包含辅料和蛋白酶变体，其中所述蛋白酶变体为 亲本蛋白酶的变体，所述亲本蛋白酶的序列与SEQ ID NO：1的氨基酸序列至少90％相同，其中蛋白酶变体的氨基酸位置根据氨基酸中相应氨基酸位置的编号进行编号 SEQ ID NO：2所示的解淀粉芽孢杆菌枯草杆菌蛋白酶BPN'的酸序列，其中所述蛋白酶变体包含选自T022A，T022R，T022Y，T022V，T022Q，T022L，T022W的突变，和（ii）漂洗和/或干燥表面 其中，所述含水液体的温度为5〜30℃。
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公开(公告)号：EP3868881A2
公开(公告)日：2021-08-25
申请号：EP20213383.1
申请日：2011-05-05
发明人： ALEKSEYEV, Viktor Yuryevich , AMIN, Neelam, S , AUGUSTYN, Katherine , CASCAO-PEREIRA, Luis Gustavo , COLLIER, Katherine, D. , ESTELL, David, A. , KELLIS JR., James, T , POULOSE, Ayrookaran, J. , SOUTER, Philip Frank , WARD, Glenn Steven , YAO, Jian
摘要： The present invention provides isolated subtilisin variants. Specifically, a subtilisin variant is a mature form having proteolytic activity and comprises an amino acid sequence comprising the combination of amino acid substitutions: T022R, S101G, S103A, V104I, A232V, and Q245R, wherein the variant is a variant of Bacillus lentus subtilisin GG36 protease comprising the amino acid sequence shown in SEQ ID NO:2, and wherein the variant has at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 2, and wherein the amino acid positions of the subtilisin variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of B. amyloliquefaciens subtilisin BPN' set forth as SEQ ID NO: 1. In addition, the present invention provides compositions comprising these subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these subtilisin variants and methods of cleaning using the cleaning compositions.
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公开(公告)号：EP3575389A3
公开(公告)日：2020-03-04
申请号：EP19182103.2
申请日：2011-05-05
发明人： SOUTER, Philip Frank , WARD, Glenn Steven , POULOSE, Ayrookaran Joseph , ESTELL, David A , KELLIS, Jr. James T. , COLLIER, Katherine D. , CASCAO-PEREIRA, Luis Gustavo , ALEKSEYEV, Viktor Yuryevich , AMIN, Neelam S. , YAO, Jian , AUGUSTYN, Katherine
摘要： A method of treating and/or cleaning a surface, preferably a fabric surface comprising the steps of (i) contacting said surface with a composition in an aqueous wash liquor, said composition comprising an adjunct material and a protease variant wherein said protease variant is a variant of a parent protease said parent protease's sequence being at least 90% identical to the amino acid sequence of SEQ ID NO:1, wherein the amino acid positions of the protease variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of Bacillus amyloliquefaciens subtilisin BPN' shown in SEQ ID NO:2, wherein said protease variant comprises a mutation selected from T022A, T022R, T022Y, T022V, T022Q, T022L, T022W, and (ii) rinsing and/or drying the surface, wherein the temperature of the aqueous liquor is from 5 to 30°C.
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公开(公告)号：EP4159833A2
公开(公告)日：2023-04-05
申请号：EP22182416.2
申请日：2010-12-09
发明人： SOUTER, Philip Frank , WARD, Glenn Steven , BASLER, Jashua Roy , CASCAO-PEREIRA, Luis Gustavo , ESTELL, David A. , KELLIS, James T. Jr. , PISARCHIK, Alexander , POULOUSE, Ayrookaran J. , TORRES-PAZMINO, Daniel , ALEKSEYEV, Viktor Yuryevich
摘要： This invention relates to fabric and home care products comprising one or more cold water proteases and processes for making and using such products. Such compositions provide improved cleaning and freshness. Such cold water proteases may be derived from parent enzymes, including BPN' subtilisin and subtilisin derived from Bacillus lentus, by substitution, insertion and/or deletion of one or more of the parent enzymes' amino acids.
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公开(公告)号：EP4141113A1
公开(公告)日：2023-03-01
申请号：EP22165362.9
申请日：2016-11-07
申请人： Danisco US Inc
发明人： ADAMS, Christian D. , ALEKSEYEV, Viktor Yuryevich , MARQUEZ, David , SCOTCHER, Miles Christopher , BOTT, Richard , YAO, Jian
摘要： Disclosed herein are mannanases from Paenibacillus sp., polynucleotides encoding the mannanases, compositions containing the mannanases, and methods of use thereof.
Compositions containing mannanases are suitable for use as detergents and for cleaning fabrics and hard surfaces, as well as in a variety of other industrial applications.
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