专利检索 ap:("The Trustees of Dartmouth College") AND inv:"LYND, Lee R." 第 1 页

1.

发明公开
PROCESS FOR THE HYDROLYSIS OF CELLULOSE MEDIATED BY TERNARY COMPLEXES OF CELLULOSE, CLOSTRIDIUM THERMOCELLUM CELLS, AND CELLULASE EXPRESSED BY THESE CELLS 审中-公开
标题翻译：方法三重纤维素复合体，热纤梭菌细胞和这些细胞表达的纤维素酶SWITCHED纤维素水解

公开(公告)号：EP2013355A2

公开(公告)日：2009-01-14

申请号：EP07811862.7

申请日：2007-05-01

申请人： The Trustees of Dartmouth College , Lynd, Lee R.

发明人： LYND, Lee R. , ZHANG, Yi-heng Percival , LU, Yanpin

IPC分类号： C12P19/14

CPC分类号： C12P19/14

摘要： Methods of utilizing reduced cellulase loads to hydrolyze cellulosic substrates are disclosed. The methods include determining an amount of purified cellulase necessary to substantially hydrolyze a quantity of cellulosic substrate in a period of time; reducing the amount of purified cellulase by a factor of between 2 and 5 to determine a reduced cellulase load; and introducing to the cellulosic substrate either (1 ) a microorganism expressing cell-bound cellulase in a concentration equal to the reduced cellulase load or (2) a fermentation agent that has been engineered to express cell-bound cellulase in a concentration equal to the reduced cellulase load under suitable conditions and for said period of time sufficient to allow substantial hydrolysis of the cellulosic substrate.

2.

发明公开
THERMOPHILIC ORGANISMS FOR CONVERSION OF LIGNOCELLULOSIC BIOMASS TO ETHANOL 审中-公开
标题翻译： THERMO AUDIOPHILE生物体在乙醇中木质纤维素生物质转化

公开(公告)号：EP2021487A2

公开(公告)日：2009-02-11

申请号：EP07783037.0

申请日：2007-05-01

申请人： The Trustees of Dartmouth College , Mascoma Corporation

发明人： SHAW, Arthur Josephus , DESAI, Sunil G. , LYND, Lee R. , TYURIN, Mikhail V. , PODKAMINER, Kara , BARDSLEY, John , HOGSETT, David Anthony

IPC分类号： C12P7/06

CPC分类号： C12N9/1029 , C12N9/0006 , C12N9/1217 , C12P7/10 , C12R1/145 , C12Y203/01008 , C12Y207/02001 , Y02E50/16 , Y02E50/17

摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALKl has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALKl, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.

3.

发明公开
RECOMBINANT YEAST STRAINS EXPRESSING TETHERED CELLULASE ENZYMES 审中-公开
标题翻译：重组酵母菌株中表达锚定纤维素酶

公开(公告)号：EP2099923A2

公开(公告)日：2009-09-16

申请号：EP07871554.7

申请日：2007-11-21

申请人： The Trustees of Dartmouth College

发明人： MCBRIDE, John E.E. , DELEAULT, Kristen M. , LYND, Lee R. , PRONK, Jack T.

IPC分类号： C12P7/10

CPC分类号： C12N9/2437 , C12N9/2445 , C12P7/10 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16

摘要： Recombinant yeast strains that saccharify, ferment and grow on insoluble and crystalline forms of cellulose are disclosed herein. The yeast strains express tethered cellulases including cellobiohydrolase, endoglucanase and β-glucosidase. The recombinant organisms are particularly suited for consolidated bioprocessing.