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公开(公告)号：EP2926830A2
公开(公告)日：2015-10-07
申请号：EP14004015.5
申请日：2011-08-31
申请人： Theraclone Sciences, Inc. , The Scripps Research Institute , International Aids Vaccine Initiative
发明人： Chan-Hui, Po-Ying , Doores, Katherine , Huber, Michael , Kaminsky, Stephen , Frey, Steven , Olsen, Ole , Mitcham, Jennifer , Moyle, Matthew , Phogat, Sanjay K. , Burton, Dennis R. , Walker, Laura Marjorie , Poignard, Pascal Raymond Georges , Koff, Wayne , Simek-Lemos, Melissa Danielle ...
CPC分类号： C07K16/1063 , A61K39/21 , A61K2039/505 , C07K16/1045 , C07K2317/21 , C07K2317/33 , C07K2317/34 , C07K2317/51 , C07K2317/515 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2317/92
摘要： The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.
摘要翻译： 本发明提供了获得广泛中和抗体（bNab）的方法，包括筛选来自供体PBMC样品的记忆B细胞培养物，以对多种HIV-1物质进行中和活性，克隆显示广泛中和活性的记忆B细胞; 并从该记忆B细胞培养物中拯救单克隆抗体。 所得到的单克隆抗体的特征在于它们以天然或单体形式选择性地结合来自Env蛋白的表位的能力，以及抑制HIV-1物种从多个进化枝的感染的能力。 提供了含有用于预防，诊断和治疗HIV感染的人单克隆抗HIV抗体的组合物。 提供了通过使用来自gp120的可变环内的保守区域的表位的免疫来产生这种抗体的方法。 还提供了产生抗艾滋病毒1型免疫球蛋白的免疫原。 此外，提供了使用合适表位的疫苗接种方法。
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2.发明公开Human immunodeficiency virus (HIV)-neutralizing antibodies 有权安非他明（Zur Neutralisierung des humanen Immundefizienz-Virus（HIV）
公开(公告)号：EP2926830A3
公开(公告)日：2015-11-11
申请号：EP14004015.5
申请日：2011-08-31
申请人： Theraclone Sciences, Inc. , The Scripps Research Institute , International Aids Vaccine Initiative
发明人： Chan-Hui, Po-Ying , Doores, Katherine , Huber, Michael , Kaminsky, Stephen , Frey, Steven , Olsen, Ole , Mitcham, Jennifer , Moyle, Matthew , Phogat, Sanjay K. , Burton, Dennis R. , Walker, Laura Marjorie , Poignard, Pascal Raymond Georges , Koff, Wayne , Simek-Lemos, Melissa Danielle ...
CPC分类号： C07K16/1063 , A61K39/21 , A61K2039/505 , C07K16/1045 , C07K2317/21 , C07K2317/33 , C07K2317/34 , C07K2317/51 , C07K2317/515 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2317/92
摘要： The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.
摘要翻译： 本发明提供了获得广泛中和抗体（bNab）的方法，包括筛选来自供体PBMC样品的记忆B细胞培养物，以对多种HIV-1物质进行中和活性，克隆显示广泛中和活性的记忆B细胞; 并从该记忆B细胞培养物中拯救单克隆抗体。 所得到的单克隆抗体的特征在于它们以天然或单体形式选择性地结合来自Env蛋白的表位的能力，以及抑制HIV-1物种从多个进化枝的感染的能力。 提供了含有用于预防，诊断和治疗HIV感染的人单克隆抗HIV抗体的组合物。 提供了通过使用来自gp120的可变环内的保守区域的表位的免疫来产生这种抗体的方法。 还提供了产生抗艾滋病毒1型免疫球蛋白的免疫原。 此外，提供了使用合适表位的疫苗接种方法。
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公开(公告)号：EP4226935A2
公开(公告)日：2023-08-16
申请号：EP23163985.7
申请日：2011-08-31
申请人： Theraclone Sciences, Inc. , The Scripps Research Institute , International AIDS Vaccine Initiative
发明人： Chan-Hui, Po-ying , Doores, Katherine , Huber, Michael , Kaminsky, Stephen , Frey, Steven , Olsen, Ole , Mitcham, Jennifer , Moyle, Matthew , Phogat, Sanjay K. , Burton, Dennis R. , Walker, Laura Marjorie , Poignard, Pascal Raymond Georges , Koff, Wayne , Simek-Lemos, Melissa Danielle, de Jean de St. Marcel
摘要： The specification shows a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV 1 bNAbs are also shown. Furthermore, methods for vaccination using suitable epitopes are shown.
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公开(公告)号：EP4226935A3
公开(公告)日：2023-09-06
申请号：EP23163985.7
申请日：2011-08-31
申请人： Theraclone Sciences, Inc. , The Scripps Research Institute , International AIDS Vaccine Initiative
发明人： Chan-Hui, Po-ying , Doores, Katherine , Huber, Michael , Kaminsky, Stephen , Frey, Steven , Olsen, Ole , Mitcham, Jennifer , Moyle, Matthew , Phogat, Sanjay K. , Burton, Dennis R. , Walker, Laura Marjorie , Poignard, Pascal Raymond Georges , Koff, Wayne , Simek-Lemos, Melissa Danielle, de Jean de St. Marcel
摘要： The specification shows a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV 1 bNAbs are also shown. Furthermore, methods for vaccination using suitable epitopes are shown.
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公开(公告)号：EP4085924A1
公开(公告)日：2022-11-09
申请号：EP22168900.3
申请日：2011-08-31
申请人： Theraclone Sciences, Inc. , The Scripps Research Institute , International AIDS Vaccine Initiative
发明人： Chan-Hui, Po-ying , Doores, Katherine , Huber, Michael , Kaminsky, Stephen , Frey, Stephen , Olsen, Ole , Mitcham, Jennifer , Moyle, Matthew , Phogat, Sanjay K. , Burton, Dennis R. , Walker, Laura Marjorie , Poignard, Pascal Raymond Georges , Koff, Wayne , Simek-Lemos, Melissa D. de Jean de St. Marcel
摘要： The specification shows a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also shown. Furthermore, methods for vaccination using suitable epitopes are shown.
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公开(公告)号：EP3438127A1
公开(公告)日：2019-02-06
申请号：EP18167464.9
申请日：2015-06-10
发明人： Burton, Dennis R. , Van Gils, J. Marit , Koff, Wayne , Poignard, Pascal Raymond Georges , Sanders, Rogier W. , Simek-Lemos, D.J.S. Melissa
IPC分类号： C07K16/10
摘要： The present invention relates to an exceptionally broad and potent neutralizing antibody which may comprise cross-clade neutralizing coverage of 83% at a median IC 50 of 0.003 µg/ml, compositions containing the same and uses thereof.
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公开(公告)号：EP1416280A3
公开(公告)日：2005-08-10
申请号：EP04000804.7
申请日：1996-09-13
IPC分类号： G01N33/543 , G01N33/563 , C07K16/00 , G01N33/577 , G01N33/68
CPC分类号： C07K16/18 , C07K2317/55 , G01N33/56983 , G01N33/6896 , G01N2800/2828
摘要： Prions are infectious pathogens that cause central nervous system spongiform encephalopathies in humans and animals. The invention relates to methods for using antibodies in detecting PrP Sc prion in samples.
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公开(公告)号：EP1416280A2
公开(公告)日：2004-05-06
申请号：EP04000804.7
申请日：1996-09-13
IPC分类号： G01N33/577 , G01N33/68
CPC分类号： C07K16/18 , C07K2317/55 , G01N33/56983 , G01N33/6896 , G01N2800/2828
摘要： Prions are infectious pathogens that cause central nervous system spongiform encephalopathies in humans and animals. The invention relates to methods for using antibodies in detecting PrP Sc prion in samples.
摘要翻译： 所要求的抗体的特征在于其能够原位结合朊病毒蛋白PrP-Sc的瘙痒病同种型。 还要求的是：（1）特异性结合PrP-Sc的抗体，其通过包括以下步骤的方法产生：（a）在噬菌体上合成抗体文库; （b）通过使噬菌体与化合物接触，对图书馆进行扫描。 包括朊蛋白（PrP）; （c）分离结合PrP-Sc蛋白的噬菌体; （2）检测源中的人PrP-Sc的方法，包括：（a） 人类PrP-Sc与特异性结合源中人类PrP-Sc 50％或更多的抗体; 和（b）确定抗体是否特异性结合源中的任何材料; 和（3）测定，包括：（a）支撑表面; 和（b）与载体表面结合的抗体，该抗体的特征在于以107l / mol或更高的结合亲和力原位结合天然PrP-Sc的能力。
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