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公开(公告)号：EP2926830A2
公开(公告)日：2015-10-07
申请号：EP14004015.5
申请日：2011-08-31
申请人： Theraclone Sciences, Inc. , The Scripps Research Institute , International Aids Vaccine Initiative
发明人： Chan-Hui, Po-Ying , Doores, Katherine , Huber, Michael , Kaminsky, Stephen , Frey, Steven , Olsen, Ole , Mitcham, Jennifer , Moyle, Matthew , Phogat, Sanjay K. , Burton, Dennis R. , Walker, Laura Marjorie , Poignard, Pascal Raymond Georges , Koff, Wayne , Simek-Lemos, Melissa Danielle ...
CPC分类号： C07K16/1063 , A61K39/21 , A61K2039/505 , C07K16/1045 , C07K2317/21 , C07K2317/33 , C07K2317/34 , C07K2317/51 , C07K2317/515 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2317/92
摘要： The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.
摘要翻译： 本发明提供了获得广泛中和抗体（bNab）的方法，包括筛选来自供体PBMC样品的记忆B细胞培养物，以对多种HIV-1物质进行中和活性，克隆显示广泛中和活性的记忆B细胞; 并从该记忆B细胞培养物中拯救单克隆抗体。 所得到的单克隆抗体的特征在于它们以天然或单体形式选择性地结合来自Env蛋白的表位的能力，以及抑制HIV-1物种从多个进化枝的感染的能力。 提供了含有用于预防，诊断和治疗HIV感染的人单克隆抗HIV抗体的组合物。 提供了通过使用来自gp120的可变环内的保守区域的表位的免疫来产生这种抗体的方法。 还提供了产生抗艾滋病毒1型免疫球蛋白的免疫原。 此外，提供了使用合适表位的疫苗接种方法。
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公开(公告)号：EP4226935A3
公开(公告)日：2023-09-06
申请号：EP23163985.7
申请日：2011-08-31
申请人： Theraclone Sciences, Inc. , The Scripps Research Institute , International AIDS Vaccine Initiative
发明人： Chan-Hui, Po-ying , Doores, Katherine , Huber, Michael , Kaminsky, Stephen , Frey, Steven , Olsen, Ole , Mitcham, Jennifer , Moyle, Matthew , Phogat, Sanjay K. , Burton, Dennis R. , Walker, Laura Marjorie , Poignard, Pascal Raymond Georges , Koff, Wayne , Simek-Lemos, Melissa Danielle, de Jean de St. Marcel
摘要： The specification shows a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV 1 bNAbs are also shown. Furthermore, methods for vaccination using suitable epitopes are shown.
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公开(公告)号：EP4085924A1
公开(公告)日：2022-11-09
申请号：EP22168900.3
申请日：2011-08-31
申请人： Theraclone Sciences, Inc. , The Scripps Research Institute , International AIDS Vaccine Initiative
发明人： Chan-Hui, Po-ying , Doores, Katherine , Huber, Michael , Kaminsky, Stephen , Frey, Stephen , Olsen, Ole , Mitcham, Jennifer , Moyle, Matthew , Phogat, Sanjay K. , Burton, Dennis R. , Walker, Laura Marjorie , Poignard, Pascal Raymond Georges , Koff, Wayne , Simek-Lemos, Melissa D. de Jean de St. Marcel
摘要： The specification shows a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also shown. Furthermore, methods for vaccination using suitable epitopes are shown.
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公开(公告)号：EP4085925A1
公开(公告)日：2022-11-09
申请号：EP22171792.9
申请日：2010-03-17
申请人： International AIDS Vaccine Initiative , Theraclone Sciences, Inc. , The Scripps Research Institute
发明人： Frey, Steven , Simek-Lemos, Melissa Danielle, de Jean de St. Marcel , Chan-Hui, Po-ying , Mitcham, Jennifer , Olsen, Ole , Moyle, Matthew , Phogat, Sanjay K. , Burton, Dennis , Walker, Laura Marjorie , Koff, Wayne , Kaminsky, Stephen
IPC分类号： A61K39/118 , A61K39/39 , C07K16/10 , A61K39/00 , A61P31/14
摘要： The invention provides a method for obtaining a broadly neutralizing antibody (bNab), wherein said antibody is PGG14 having a heavy chain comprising the amino acid sequence of SEQ ID NO: 20 and a light chain comprising the amino acid sequence of SEQ ID NO: 22. Said method includes screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibody of the invention is characterized by its ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing said human monoclonal anti-HIV antibody of the invention, which are used for prophylaxis, diagnosis and treatment of HIV infection, are provided. Methods for generating such antibody by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIVl bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.
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公开(公告)号：EP3260136A1
公开(公告)日：2017-12-27
申请号：EP17173548.3
申请日：2010-03-17
申请人： Theraclone Sciences, Inc. , The Scripps Research Institute , International AIDS Vaccine Initiative
发明人： Chan-Hui, Po-ying , Mitcham, Jennifer , Olsen, Ole , Frey, Steven , Moyle, Matthew , Phogat, Sanjay K. , Burton, Dennis , Walker, Laura Marjorie , Poignard, Pascal Raymond Georges , Simek-Lemos, Melissa Danielle, de Jean de St. Marcel , Koff, Wayne , Kaminsky, Stephen
IPC分类号： A61K39/118 , A61K39/39 , C07K16/10
CPC分类号： C07K16/1045 , A61K39/21 , A61K2039/505 , C07K16/1063 , C07K2317/56 , C07K2317/565 , C07K2317/76
摘要： The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies are characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.
摘要翻译： 本发明提供了获得广泛中和抗体（bNab）的方法，包括筛选来自供体PBMC样品的记忆B细胞培养物的针对多种HIV-1物种的中和活性，克隆展现广泛中和活性的记忆B细胞; 并从该记忆B细胞培养物中拯救单克隆抗体。 所得单克隆抗体的特征在于其以天然或单体形式选择性结合来自Env蛋白的表位的能力，以及抑制来自多个进化枝的HIV-1物种的感染。 提供了含有用于预防，诊断和治疗HIV感染的人单克隆抗HIV抗体的组合物。 提供了使用来自gp120的可变环内的保守区的表位进行免疫来产生此类抗体的方法。 还提供了用于产生抗HIV1bNAb的免疫原。 此外，提供了使用合适表位的疫苗接种方法。
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6.发明公开Human immunodeficiency virus (HIV)-neutralizing antibodies 有权安非他明（Zur Neutralisierung des humanen Immundefizienz-Virus（HIV）
公开(公告)号：EP2926830A3
公开(公告)日：2015-11-11
申请号：EP14004015.5
申请日：2011-08-31
申请人： Theraclone Sciences, Inc. , The Scripps Research Institute , International Aids Vaccine Initiative
发明人： Chan-Hui, Po-Ying , Doores, Katherine , Huber, Michael , Kaminsky, Stephen , Frey, Steven , Olsen, Ole , Mitcham, Jennifer , Moyle, Matthew , Phogat, Sanjay K. , Burton, Dennis R. , Walker, Laura Marjorie , Poignard, Pascal Raymond Georges , Koff, Wayne , Simek-Lemos, Melissa Danielle ...
CPC分类号： C07K16/1063 , A61K39/21 , A61K2039/505 , C07K16/1045 , C07K2317/21 , C07K2317/33 , C07K2317/34 , C07K2317/51 , C07K2317/515 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2317/92
摘要： The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.
摘要翻译： 本发明提供了获得广泛中和抗体（bNab）的方法，包括筛选来自供体PBMC样品的记忆B细胞培养物，以对多种HIV-1物质进行中和活性，克隆显示广泛中和活性的记忆B细胞; 并从该记忆B细胞培养物中拯救单克隆抗体。 所得到的单克隆抗体的特征在于它们以天然或单体形式选择性地结合来自Env蛋白的表位的能力，以及抑制HIV-1物种从多个进化枝的感染的能力。 提供了含有用于预防，诊断和治疗HIV感染的人单克隆抗HIV抗体的组合物。 提供了通过使用来自gp120的可变环内的保守区域的表位的免疫来产生这种抗体的方法。 还提供了产生抗艾滋病毒1型免疫球蛋白的免疫原。 此外，提供了使用合适表位的疫苗接种方法。
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公开(公告)号：EP3260136B1
公开(公告)日：2020-11-25
申请号：EP17173548.3
申请日：2010-03-17
申请人： Theraclone Sciences, Inc. , The Scripps Research Institute , International AIDS Vaccine Initiative
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公开(公告)号：EP4226935A2
公开(公告)日：2023-08-16
申请号：EP23163985.7
申请日：2011-08-31
申请人： Theraclone Sciences, Inc. , The Scripps Research Institute , International AIDS Vaccine Initiative
发明人： Chan-Hui, Po-ying , Doores, Katherine , Huber, Michael , Kaminsky, Stephen , Frey, Steven , Olsen, Ole , Mitcham, Jennifer , Moyle, Matthew , Phogat, Sanjay K. , Burton, Dennis R. , Walker, Laura Marjorie , Poignard, Pascal Raymond Georges , Koff, Wayne , Simek-Lemos, Melissa Danielle, de Jean de St. Marcel
摘要： The specification shows a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV 1 bNAbs are also shown. Furthermore, methods for vaccination using suitable epitopes are shown.
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公开(公告)号：EP3323427A1
公开(公告)日：2018-05-23
申请号：EP17194834.2
申请日：2010-03-17
申请人： Theraclone Sciences, Inc. , The Scripps Research Institute , International AIDS Vaccine Initiative
发明人： Chan-Hui, Po-ying , Mitcham, Jennifer , Olsen, Ole , Frey, Steven , Moyle, Matthew , Phogat, Sanjay K. , Burton, Dennis , Walker, Laura Marjorie , de Jean de St. Marcel Simek-Lemos, Melissa D. , Koff, Wayne , Kaminsky, Stephen
IPC分类号： A61K39/118 , A61K39/39 , C07K16/10 , A61K39/00
CPC分类号： C07K16/1045 , A61K39/21 , A61K2039/505 , C07K16/1063 , C07K2317/56 , C07K2317/565 , C07K2317/76
摘要： The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies are characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV 1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.
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公开(公告)号：EP3178489A1
公开(公告)日：2017-06-14
申请号：EP16206293.9
申请日：2011-08-31
申请人： Theraclone Science, Int. , The Scripps Research Institute , International AIDS Vaccine Initiative
发明人： Chan-Hui, Po-ying , Doores, Katherine , Huber, Michael , Kaminsky, Stephen , Frey, Steven , Olsen, Ole , Mitcham, Jennifer , Moyle, Matthew , Phogat, Sanjay K. , Burton, Dennis , Walker, Laura Marjorie , Poignard, Pascal Raymond Georges , Koff, Wayne , Simek-Lemos, Melissa Danielle, de Jean de St. Marcel
CPC分类号： C07K16/1063 , A61K39/21 , A61K2039/505 , C07K16/1045 , C07K2317/21 , C07K2317/33 , C07K2317/34 , C07K2317/51 , C07K2317/515 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2317/92
摘要： The specification shows a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also shown. Furthermore, methods for vaccination using suitable epitopes are shown. More specifically, the invention relates to a broadly neutralizing antibody (bNAb) comprising the polypeptide 4869-K15 (PGT-133), i.e to a monoclonal anti-HIV antibody comprising a heavy chain sequence comprising the amino acid sequence of SEQ ID NO: 420 and a light chain sequence comprising the amino acid sequence SEQ ID NO: 429.
摘要翻译： 该说明书显示了获得广泛中和抗体（bNab）的方法，包括从供体PBMC样品中筛选记忆B细胞培养物以对多种HIV-1物质的中和活性，克隆显示广泛中和活性的记忆B细胞; 并从该记忆B细胞培养物中拯救单克隆抗体。 所得到的单克隆抗体的特征在于它们以天然或单体形式选择性地结合来自Env蛋白的表位的能力，以及抑制HIV-1物种从多个进化枝的感染的能力。 提供了含有用于预防，诊断和治疗HIV感染的人单克隆抗HIV抗体的组合物。 提供了通过使用来自gp120的可变环内的保守区域的表位的免疫来产生这种抗体的方法。 还显示了用于产生抗HIV1bNAbs的免疫原。 此外，示出了使用合适表位的疫苗接种方法。 更具体地，本发明涉及包含多肽4869-K15（PGT-133）的广泛中和抗体（bNAb），即涉及含有包含SEQ ID NO：420的氨基酸序列的重链序列的单克隆抗HIV抗体 和包含氨基酸序列SEQ ID NO：429的轻链序列。
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