Method and reagent for determination of dehydrogenase or its substrate
    4.
    发明公开
    Method and reagent for determination of dehydrogenase or its substrate 失效
    用于测定脱氢酶或其底物的方法和试剂

    公开(公告)号:EP0285998A3

    公开(公告)日:1990-06-13

    申请号:EP88105172.6

    申请日:1988-03-30

    IPC分类号: C12Q1/32 C12Q1/28

    CPC分类号: C12Q1/28 C12Q1/32

    摘要: A method for determining dehydrogenase or its substrate in dehydrogenase reaction, which comprises subjecting a dehydrogenase and its substrate to a dehydro­genase reaction in the presence of an electron carrier or a combination of a coenzyme and an electron carrier, allowing to form H₂O₂ through transfer of electrons derived from the substrate by the dehydrogenase reaction to O₂ via the electron carrier or via the coenzyme and electron carrier, stopping the dehydrogenase reaction, reacting said H₂O₂ with a peroxidase and a chromogen, and measuring a formed dye, and a reagent for determining dehydrogenase or its substrate. By the method and the reagent, dehydrogenase or its substrate can be accurately determined in a short time regardless of the concentration of the dehydrogenase and/or the substrate and without staining tubes or cells of a determination device with a dye.

    Maltooligosaccharide derivatives and reagents for determination of amylase activity
    6.
    发明公开
    Maltooligosaccharide derivatives and reagents for determination of amylase activity 失效
    麦芽糖糖苷衍生物和最佳成分。

    公开(公告)号:EP0319993A2

    公开(公告)日:1989-06-14

    申请号:EP88120609.8

    申请日:1988-12-09

    IPC分类号: C07H15/203 C08B33/04 C12Q1/40

    CPC分类号: C07H15/203

    摘要: Maltooligosaccharide derivatives represented by the general formula:
    wherein at least one of R¹ and R² denotes a radical represented by: R³-S(O) m -R⁴ or R³-CO-R⁴, and the rest of them denotes hydrogen atom, an alkyl radical containing 1 to 6 carbon atoms or a phenyl radical, in which R³ is an alkyl radical containing 1 to 5 carbon atoms, R⁴ is an alkylene radical containing 1 to 5 carbon atoms, and m is 1 or 2, R⁵ denotes a substituted or non-substituted phenyl radical, and n denotes an integer of 1 to 8 ; and reagents comprising the maltooligosaccharide derivatives.

    摘要翻译: 由下式通式表示的麦芽低聚糖衍生物:其中R 1和R 2中的至少一个表示由以下基团表示的基团:R 3 -S(O)m R 4或R 3 - CO-R 4,其余的表示氢原子,含有1至6个碳原子的烷基或苯基,其中R 3是含有1至5个碳原子的烷基,R 4 是含有1至5个碳原子的亚烷基,m是1或2,R 5表示取代或未取代的苯基,n表示1至8的整数; 和包含麦芽低聚糖衍生物的试剂。

    Method and reagent for determination of dehydrogenase or its substrate
    9.
    发明公开
    Method and reagent for determination of dehydrogenase or its substrate 失效
    Verfahren und Reagens zur Bestimmung von Dehydrogenase oder ihrem Substrat。

    公开(公告)号:EP0285998A2

    公开(公告)日:1988-10-12

    申请号:EP88105172.6

    申请日:1988-03-30

    IPC分类号: C12Q1/32 C12Q1/28

    CPC分类号: C12Q1/28 C12Q1/32

    摘要: A method for determining dehydrogenase or its substrate in dehydrogenase reaction, which comprises subjecting a dehydrogenase and its substrate to a dehydro­genase reaction in the presence of an electron carrier or a combination of a coenzyme and an electron carrier, allowing to form H₂O₂ through transfer of electrons derived from the substrate by the dehydrogenase reaction to O₂ via the electron carrier or via the coenzyme and electron carrier, stopping the dehydrogenase reaction, reacting said H₂O₂ with a peroxidase and a chromogen, and measuring a formed dye, and a reagent for determining dehydrogenase or its substrate. By the method and the reagent, dehydrogenase or its substrate can be accurately determined in a short time regardless of the concentration of the dehydrogenase and/or the substrate and without staining tubes or cells of a determination device with a dye.

    摘要翻译: 一种用于在脱氢酶反应中测定脱氢酶或其底物的方法,其包括在电子载体或辅酶和电子载体的组合的存在下使脱氢酶及其底物进行脱氢酶反应,允许通过电子传递形成H 2 O 2 通过脱氢酶通过电子载体或通过辅酶和电子载体对O 2进行脱氢酶反应,停止脱氢酶反应,使所述H 2 O 2与过氧化物酶和色原反应,并测量形成的染料,以及用于测定脱氢酶或 其底物。 通过该方法和试剂,可以在短时间内精确地测定脱氢酶或其底物,而不管脱氢酶和/或底物的浓度如何,而不用用染料染色的测定装置的管或细胞染色。