公开(公告)号：EP0683790A4

公开(公告)日：1996-03-13

申请号：EP94907420

申请日：1994-02-04

申请人： UNIV GEORGIA RES FOUND

发明人： MCGRAW ROYAL A ,  GROSSE WILLIAM MICHAEL

IPC分类号： C12M1/00 ,  B01J19/00 ,  C07K1/04 ,  C12M1/36 ,  C40B40/06 ,  C40B60/14 ,  C07K17/00 ,  G05D7/00

CPC分类号： B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00308 ,  B01J2219/00364 ,  B01J2219/00416 ,  B01J2219/00418 ,  B01J2219/00423 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14

摘要： Apparatus and method for the automated synthesis of DNA segments utilizing multiple reaction columns (11), all of which are open at the inlet end (13) to the atmosphere of a reaction chamber (10). A moveable reagent supply line outlet (12) is positioned adjacent to the column inlet end (13) to apply reagent to each of the columns (11) according to an input sequence of delivery. The delivery sequence is under processor control. Reagents are removed from all columns (11) simultaneously through the application of vacuum (22) at the outlet end (14) of each column (11). The device enables the parallel synthesis of large numbers of different oligonucleotide sequences of different lengths.