THREE-DIMENSIONAL IMAGE CONSTRUCTION METHOD, IMAGE PROCESSING DEVICE, AND ELECTRON MICROSCOPE

    公开(公告)号:EP3185000A4

    公开(公告)日:2018-02-14

    申请号:EP15833770

    申请日:2015-08-21

    摘要: A three-dimensional image reconstruction method capable of analyzing a three-dimensional structure of membrane proteins present within a lipid membrane is offered. A three-dimensional image reconstruction method associated with the present invention comprises the steps of: obtaining a first transmission electron microscope image of a sample containing the membrane proteins present within a lipid membrane, the image having been taken by illuminating an electron beam on the sample from a direction tilted relative to a line normal to the membrane surface of the lipid membrane (step S10); obtaining a second transmission electron microscope image of the sample taken by illuminating the electron beam on the sample perpendicularly to the membrane surface of the lipid membrane (step S12); identifying orientations of the membrane proteins of the first transmission electron microscope image on a basis of the second transmission electron microscope image (step S14); and analyzing a three-dimensional structure of the membrane proteins from the first transmission electron microscope image on a basis of information about the identified orientations of the membrane proteins (step S18).