公开(公告)号：EP0267951A4

公开(公告)日：1989-02-23

申请号：EP87903802

申请日：1987-05-21

申请人： UNIV NEW YORK

发明人： GITEL SANFORD ,  WESSLER STANFORD

IPC分类号： G01N33/53 ,  C08G73/00 ,  C12Q1/00 ,  C12Q1/28 ,  C12Q1/42 ,  G01N33/86 ,  G01N33/94

CPC分类号： G01N33/86 ,  G01N33/94 ,  G01N2400/40 ,  Y10S435/81 ,  Y10S436/808 ,  Y10S436/815

摘要： Assay for measuring heparin in a biological fluid by incubating the fluid in the presence of an immobilized heparin inhibitor (preferably a positively charged polymer of 1-ethyl-3-(3-dimethylaminopropyl)carboiimide) causing the heparin to bind to the inhibitor forming a 2-component complex; incubating the two-component complex with an anti-heparin antibody causing the antibody to bind to the complex forming a three-component heparin/inhibitor/anti-heparin complex; incubating said three-component complex with detectable second antibody recognizing said anti-heparin antibody forming a four-component heparin/inhibitor/anti-heparin (second antibody) complex and detecting the amount of said second antibody in said four-component complex.