公开(公告)号：EP2139515A4

公开(公告)日：2011-07-06

申请号：EP08799746

申请日：2008-03-31

申请人： UNIV NEW YORK STATE RES FOUND

发明人： WIMMER ECKARD ,  SKIENA STEVE ,  MUELLER STEFFEN ,  FUTCHER BRUCE ,  PAPAMICHAIL DIMITRIS ,  COLEMAN JOHN ROBERT ,  CELLO JERONIMO

IPC分类号： A61K39/13

CPC分类号： C12N7/00 ,  A61K39/13 ,  A61K39/145 ,  A61K2039/5254 ,  C07K14/005 ,  C12N2720/12361 ,  C12N2740/15061 ,  C12N2760/16061 ,  C12N2760/16121 ,  C12N2760/16134 ,  C12N2760/16162 ,  C12N2770/20061 ,  C12N2770/24161 ,  C12N2770/32061 ,  C12N2770/32621 ,  C12N2770/32634 ,  C12N2770/32661 ,  C12N2770/32662 ,  C12N2770/32671 ,  C12N2770/32721 ,  C12N2770/32761 ,  C12N2770/32762

摘要： This invention provides an attenuated virus which comprises a modified viral genome containing nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce synonymous deoptimized codons into the genome. The instant attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the instant attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the instant attenuated virus.