公开(公告)号：EP3212757A4

公开(公告)日：2018-07-04

申请号：EP15855382

申请日：2015-10-27

申请人： GOVERNING COUNCIL UNIV TORONTO

发明人： SIMMONS CRAIG ,  CHEBOTAREV OLEG

IPC分类号： C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12N5/00 ,  G01N33/48

CPC分类号： G01N33/5088 ,  B01L3/5027 ,  B01L3/5085 ,  B01L2200/0689 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2400/049 ,  C12M23/12 ,  C12M23/16 ,  C12M23/40 ,  C12M25/02 ,  C12M29/10 ,  C12N5/0075 ,  G01N33/5058 ,  G01N33/5067 ,  G01N2500/10

摘要： A microfluidic device, method and kit for assaying and/or culturing cells are provided. The microfluidic device comprises a well block comprising a plurality of microwells; at least one cell culture layer selected from a first cell culture layer comprising a plurality of microchannels, each microchannel being aligned with one of the plurality of microwells and being in fluid communication with the aligned microwells; and a second cell culture layer comprising a plurality of cell culture chamber wells, each cell culture chamber well being aligned with one of the plurality of microwells and being in fluid communication with the aligned microwells, and a plurality of outlets, each of the plurality of outlets corresponding to one of the plurality of cell culture chamber wells; and a base block, wherein the at least one cell culture layer is sealably coupled between the well block and the base block, thereby allowing fluid communication between the plurality of microwells in the well block and the at least one cell culture layer.

公开(公告)号：EP2198168A4

公开(公告)日：2013-12-25

申请号：EP08800383

申请日：2008-09-29

申请人： UNIV TORONTO

发明人： MORAES CHRISTOPHER ,  SIMMONS CRAIG ,  SUN YU

IPC分类号： F15B5/00 ,  B81B3/00 ,  B81B7/04 ,  C12M1/00 ,  C12M1/12 ,  C12Q1/02 ,  C40B30/06 ,  C40B30/10 ,  G01N19/00

CPC分类号： C12M23/16 ,  C12M25/04 ,  C12M35/04

摘要： The present invention details the design and operation of a miniaturized device array in which a range of simultaneous mechanical forces are produced by a single external pressure source. The invention is primarily embodied in a microfabricated device arrays designed to rapidly probe biological cell response to various combinations of mechanical, chemical and extra-cellular matrix parameters in a high-throughput fashion.