公开(公告)号：EP3293262A1

公开(公告)日：2018-03-14

申请号：EP16188155.2

申请日：2016-09-09

申请人： Universität Potsdam

发明人： MESSERSCHMIDT, Katrin ,  MACHENS, Fabian ,  HOCHREIN, Lena ,  MÜLLER-RÖBER, Bernd

IPC分类号： C12N15/10 ,  C12N15/65 ,  C12N15/66

CPC分类号： C12N15/1093 ,  C12N15/1027 ,  C12N15/1031 ,  C12N15/65 ,  C12N15/66

摘要： Described is a method for generating a multiple-gene assembly in a single DNA construct, a kit and a single DNA construct generated by the method.
Different assembly methods are known in the state of the art but user has to decide between scar free, sequence independent methods, which require the time consuming individual design of every assembly and modular approaches, which generate scar sequences and require laborious parts domestication.
In order to overcome these drawbacks a method for generating a multiple-gene assembly in a single DNA construct is provided that contains freely designed scar-free level 0 assembly units in a predefined order, determined by flanking homology regions and which is sequence-independent.

摘要翻译： 描述了用于在单个DNA构建体，试剂盒和通过该方法产生的单个DNA构建体中产生多基因组装体的方法。 现有技术中已知不同的组装方法，但用户必须在无疤痕，不依赖序列的方法之间做出决定，这些方法需要每个组件的耗时的单独设计和模块化方法，这会产生瘢痕序列并且需要费力的部件驯化。 为了克服这些缺点，提供了用于在单个DNA构建体中产生多基因组装体的方法，其包含以预定顺序自由设计的无疤痕0级组装单元，其由侧翼同源性区域确定并且是序列无关的。