公开(公告)号：EP2604682A1

公开(公告)日：2013-06-19

申请号：EP11306636.9

申请日：2011-12-12

申请人： Universite Joseph Fourier - Grenoble 1 ,  Centre Hospitalier Universitaire de Grenoble

发明人： Le Gouellec, Audrey ,  Toussaint, Bertrand ,  Polack, Benoît

IPC分类号： C12N1/20 ,  C12R1/38 ,  A61K39/00

CPC分类号： C12N1/36 ,  A61K39/0005 ,  A61K39/0011 ,  A61K2039/522 ,  A61K2039/523 ,  C07K14/435 ,  C12N1/20 ,  C12N15/102 ,  C12N15/78 ,  C12R1/38

摘要： The invention is related to a process for obtaining an adapted strain of Pseudomonas, comprising the following steps:
• deleting the genes ExoS ExoT, aroA and lasI in an initial Pseudomonas strain cultivated in a LB medium,
• progressively cultivating this strain in a chemically defined medium based on a glucose minimal medium supplemented with magnesium and calcium,

wherein the adapted strain presents the same toxicity and secretion capacities than the initial strain, and its doubling time when cultivated in said chemically defined medium is less than 60 minutes.
The present invention is also related to the adapted strain such as obtained,in particular wherein the adapted strain is furthermore treated to become 'killed but metabolically active'.

摘要翻译： 本发明涉及一种方法，用于获得假单胞菌的angepasst应变的方法，包括以下步骤：在LB培养基中培养初始假单胞菌菌株在€¢删除基因EXOS ExoT，的aroA和椰子，€¢在逐步培养该菌株 化学成分确定的培养基基于补充有镁和钙，所述worin应变angepasst呈现比初始菌株相同的毒性和分泌能力葡萄糖基本培养基上，并确定了其倍增时间当所述化学培养介质是少于60分钟。 因此，本发明是有关angepasst应变：如获得特别worin的angepasst菌株更进一步处理以变成“杀死但具有代谢活性”。