公开(公告)号：EP2422356A1

公开(公告)日：2012-02-29

申请号：EP10715458.5

申请日：2010-04-22

申请人： Massachusetts Institute of Technology ,  Whitehead Institute For Biomedical Research ,  University Of Nottingham

发明人： SAHA, Krishanu ,  MEI, Ying ,  BOGATYREV, Said, R. ,  ANDERSON, Daniel, G. ,  JAENISCH, Rudolf ,  LANGER, Robert, S. ,  ALEXANDER, Morgan ,  DAVIES, Martyn ,  YANG, Jing ,  KASTRUP, Christian, J. ,  URQUHART, Andrew

IPC分类号： H01J49/14

CPC分类号： G01N23/2258 ,  C12N5/0068 ,  C12N2535/10 ,  H01J49/00

摘要： The present disclosure provides a device and a cell culture system comprising a substrate that generates significant chemical ion signatures adapted for cultυring stem cells. This disclosure further provides unique surface properties, such as surface wettability, along with defined polymer microspot environments in an array, for effectively supporting the propagation and differentiation of human pluripotent stem cells in vitro. Methods of cυlturing, maintenance, differentiating stem cells as well as reprogramming somatic cells into stem cells using the device and the cell culture system with the suitable substrates, along with suitable culture media, are also provided.

公开(公告)号：EP2175980A2

公开(公告)日：2010-04-21

申请号：EP08788645.3

申请日：2008-08-07

申请人： University Of Nottingham ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： DAVIES, Martyn ,  ALEXANDER, Morgan ,  WILLIAMS, Paul ,  LANGER, Robert ,  ANDERSON, Daniel ,  URQUHART, Andrew

IPC分类号： B01J19/00 ,  C40B50/14

CPC分类号： C40B50/14 ,  B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00691 ,  B01J2219/00711 ,  B01J2219/00716 ,  B01J2219/00736 ,  B01J2219/00743 ,  B82Y30/00 ,  C12Q1/04

摘要： This invention relates to a method of screening arrays of polymers having pre- determined surface energies. The polymer arrays of the present invention can be used to screen for microorganism adherence. More specifically the arrays can be used to screen for adherence of particular bacteria or fungi to particular polymers in the array. Furthermore, this invention relates to a method combining in-situ polymer synthesis with physico-chemical characterisation of the resulting polymer array and subsequent biological assays of bacterial or fungal adherence. This allows for high throughput screening and characterisation of candidate polymers which are not susceptible to bacterial or fungal adherence or which can be used to support bacterial or fungal adherence where such is required. The arrays can also be used to screen for inhibition or promotion of biofilm formation.